公开(公告)号：EP0922097A1

公开(公告)日：1999-06-16

申请号：EP97913984.0

申请日：1997-03-27

申请人： COMMONWEALTH SCIENTIFIC AND INDUSTRIAL RESEARCH ORGANISATION ,  THE AUSTRALIAN NATIONAL UNIVERSITY

发明人： SURIN, Brian, Peter ,  DE FEYTER, Robert, Charles ,  GRAHAM, Michael, Wayne ,  WATERHOUSE, Peter, Michael ,  KEESE, Paul, Konrad ,  ALI, Shahjahan

IPC分类号： C12N15 ,  A01H5 ,  C12N5

CPC分类号： C12N15/8217 ,  C12N15/8209 ,  C12N15/8213 ,  C12N15/8242 ,  C12N15/8265

摘要： The present invention is directed to the genetic transformation using multiple genetic sequences, wherein one of said genetic sequences encodes a polypeptide possessing excision activity, specifically a site-specific recombinase activity linked to a transgene unit and the use of this genetic construct in the removal of transgenes therefrom. The present invention provides the means to produce genetically-transformed organisms, in particular plants, in which selectable marker genes have been removed, thereby facilitating multiple sequential genetic transformation events using the same selectable marker gene. Accordingly, the invention provides the means for regulating transgene expression in genetically-manipulated organisms, for example to promote differentiation, de-differentiation, or any unidirectional developmental shift of a target cell which requires the time-specific expression of a particular gene. The invention is particularly suited to the promotion of specific organogeneses in plants using organogenesis-promoting transgenes, wherein the organs which subsequently develop in said plants are genetically transformed with a desired gene but lack organogenesis-promoting transgenes.