公开(公告)号：EP3000889B1

公开(公告)日：2018-12-05

申请号：EP15188570.4

申请日：2010-03-01

申请人： Evogene Ltd.

发明人： EMMANUEL, Eyal ,  DIBER, Alex ,  POLLOCK, Sarah Rachel ,  KARCHI, Hagai

IPC分类号： C12N15/82 ,  C07K14/415

CPC分类号： C12N15/8271 ,  C07K14/415 ,  C12N15/8209 ,  C12N15/8247 ,  C12N15/8255 ,  C12N15/8261 ,  C12N15/8273 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/158 ,  Y02A40/146

摘要： Provided are isolated polynucleotides which comprise a nucleic acid sequence at least 80% identical to SEQ ID NO: 3487, 1-239, 467-1973, 3481-3486, 3488-3674, 3738 or 3739; isolated polypeptides which comprise an amino acid sequence at least 80% homologous to SEQ ID NO: 246, 240-245, 247-465, 1974-3480, 3675-3736 or 3737, and methods of using same for increasing a yield, biomass, growth rate, vigor, oil content, fiber yield, fiber quality, abiotic stress tolerance, and/or nitrogen use efficiency of a plant.

公开(公告)号：EP3059318B1

公开(公告)日：2018-09-05

申请号：EP16156286.3

申请日：2016-02-18

申请人： SUMITOMO RUBBER INDUSTRIES, LTD.

发明人： INOUE, Yukino ,  OKADA, Akari ,  YAMAGUCHI, Haruhiko ,  KURODA, Satoshi

IPC分类号： C12N15/82 ,  C12N9/10 ,  C07K14/415 ,  C12P5/00 ,  A01H5/00

CPC分类号： C12N15/8243 ,  C07K14/415 ,  C12N9/0006 ,  C12N9/1085 ,  C12N9/90 ,  C12N15/8209 ,  C12N15/8223 ,  C12P5/007 ,  C12Y101/01034 ,  C12Y205/0101 ,  C12Y205/0102 ,  C12Y205/01029 ,  C12Y503/03002

摘要： Provided is a vector capable of improving polyisoprenoid production through the introduction of the vector into a plant using gene recombination techniques. A vector comprising: a promoter of a gene encoding Small Rubber Particle Protein; and a gene encoding a protein involved in polyisoprenoid biosynthesis, the gene being functionally linked to the promoter.

公开(公告)号：EP3007547B1

公开(公告)日：2018-07-18

申请号：EP14811564.5

申请日：2014-06-06

申请人： Syngenta Participations AG

发明人： LI, Xianggan ,  ELUMALAI, Sivamani

IPC分类号： A01H5/00 ,  C12N15/82 ,  C12P21/06 ,  C12N5/04

CPC分类号： C12N15/8201 ,  C12N15/8205 ,  C12N15/8209

摘要： This invention provides a method for generating transgenic plants with a low copy number. Plant cells are transformed with polynucleotides containing transcriptional cassettes designed to trigger silencing of a gene which is essential for the plant cell to survive the transformation and regeneration process. The present invention enables the recovery of an increased number of transgenic plants which have only one copy of each desired transcriptional cassette.

公开(公告)号：EP3240889A4

公开(公告)日：2018-06-20

申请号：EP15876386

申请日：2015-12-31

申请人： SYNTHETIC GENOMICS INC

发明人： VERRUTO JOHN ,  MOELLERING ERIC

IPC分类号： C12N5/00 ,  C12N15/00 ,  C12N15/81 ,  C12N15/82

CPC分类号： C12N15/8213 ,  C12N9/1241 ,  C12N9/22 ,  C12N15/8209 ,  C12N15/8216 ,  C12N15/85 ,  C12N15/90 ,  C12N15/905 ,  C12N15/907 ,  C12Y207/07

摘要： The present invention provides cell lines for high efficiency genome editing using cas/CRISPR systems, methods of generating such cells lines, and methods of generating mutations in the genome of an organism using such cell lines.

公开(公告)号：EP3240889A2

公开(公告)日：2017-11-08

申请号：EP15876386.2

申请日：2015-12-31

申请人： Synthetic Genomics, Inc.

发明人： VERRUTO, John ,  MOELLERING, Eric

IPC分类号： C12N5/00 ,  C12N15/00 ,  C12N15/81 ,  C12N15/82

CPC分类号： C12N15/8213 ,  C12N9/1241 ,  C12N9/22 ,  C12N15/8209 ,  C12N15/8216 ,  C12N15/85 ,  C12N15/90 ,  C12N15/905 ,  C12N15/907 ,  C12Y207/07

摘要： The present invention provides cell lines for high efficiency genome editing using cas/CRISPR systems, methods of generating such cells lines, and methods of generating mutations in the genome of an organism using such cell lines.

公开(公告)号：EP3234154A1

公开(公告)日：2017-10-25

申请号：EP15817189.2

申请日：2015-12-15

申请人： Paris Sciences et Lettres - Quartier Latin ,  Institut National de la Santé et de la Recherche Médicale (INSERM) ,  Centre National de la Recherche Scientifique (CNRS)

发明人： NAVARRO, Lionel ,  THIEBEAULD, Odon

IPC分类号： C12N15/82 ,  C12N15/113 ,  C12Q1/68

CPC分类号： C12N15/8209 ,  C12N15/113 ,  C12N15/65 ,  C12N15/8212 ,  C12N15/8216 ,  C12N15/8218 ,  C12N15/8223 ,  C12N15/8241 ,  C12N15/8279 ,  C12Q1/6897 ,  C12Q2600/13

摘要： The invention relates to transgenic plants comprising an inverted-repeat construct which triggers post-transcriptional gene silencing of an endogenous visual reporter gene driven by a tissue-specific promoter wherein said tissue is relevant for pathogen entry, propagation or replication and their uses for screening natural or synthetic molecules, microorganisms or extracts from micro- or macro-organisms for their potential ability to inhibit pathogen entry, propagation or replication in plants by enhancing PTGS or for characterizing the mode of action of natural or synthetic molecules that are known to enhance plant disease resistance through an ill-defined mode of action.

摘要翻译： 本发明涉及包含反转重复构建体的转基因植物，所述反转重复构建体触发由组织特异性启动子驱动的内源视觉报道基因的转录后基因沉默，其中所述组织与病原体进入，繁殖或复制相关并且其用于筛选天然 或合成分子，来自微生物或大生物的微生物或提取物，因为它们通过增强PTGS或抑制已知会增强植物病害的天然或合成分子的作用模式来抑制植物中病原体进入，繁殖或复制的潜在能力 通过一个不明确的行动模式来抵抗。

公开(公告)号：EP3103870A4

公开(公告)日：2017-09-20

申请号：EP15745845

申请日：2015-02-04

申请人： JAPAN TOBACCO INC

发明人： HIEI YUKOH ,  KOMARI TOSHIHIKO

IPC分类号： C12N15/09 ,  A01H1/00 ,  C12N5/10

CPC分类号： C12N15/821 ,  C12N15/8209 ,  C12N2830/002 ,  C12N2830/003 ,  C12N2830/006 ,  C12Q1/6895

摘要： The present invention relates to a method of obtaining a transformed plant cell. The present invention comprises the steps of: (a) co-transforming an intended DNA and a first marker gene into a plant cell; and (b) selecting from the transformed cells obtained in the step (a), a transformed plant cell wherein the intended DNA is introduced into a chromosome thereof, and the first marker gene is not introduced , wherein the method does not contain a step to exclude a transformed cell with only the intended DNA introduced into the chromosome by positive selection using the first marker gene.

摘要翻译： 本发明涉及获得转化的植物细胞的方法。 本发明包括以下步骤：（a）将预期的DNA和第一标记基因共转化到植物细胞中; （b）从步骤（a）中获得的转化细胞中选择其中将目的DNA导入其染色体中并且不导入第一标记基因的转化植物细胞，其中该方法不包含步骤 通过使用第一标记基因的正向选择仅将预期的DNA导入染色体中排除转化的细胞。

公开(公告)号：EP3011039A4

公开(公告)日：2017-01-25

申请号：EP14814381

申请日：2014-05-20

申请人： UNIV HONG KONG

发明人： CHYE MEE LEN ,  LIAO PAN ,  WANG HUI ,  WANG MINGFU

IPC分类号： C12N15/82 ,  A01H5/00 ,  C12N1/21

CPC分类号： C12N15/8261 ,  C12N9/88 ,  C12N15/8209 ,  C12N15/8222 ,  C12N15/8243 ,  C12N15/8247 ,  C12Y203/0301 ,  Y02A40/146

摘要： Provided herein is a transgenic plant, seed, or progeny, genetically engineered to overexpress one or more exogenous 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGS1) in an amount effective to enhance growth and/or seed yield relative to a control plant. Also provided are methods of enhancing plant growth and/or seed yield by genetically engineering a plant to overexpress one or more exogenous HMGS1 in an amount effective to enhance growth and/or seed yield relative to a control plant. The plant belongs to the Solanaceae family, and the one or more exogenous HMGS1 comprise an amino acid sequence at least 77% identical to Brassica juncea HMGS1 as set forth in SEQ ID NO:6. Further provided are methods of screening for a functional variant of Brassica juncea HMGS1.

公开(公告)号：EP3059318A1

公开(公告)日：2016-08-24

申请号：EP16156286.3

申请日：2016-02-18

申请人： SUMITOMO RUBBER INDUSTRIES, LTD.

发明人： INOUE, Yukino ,  OKADA, Akari ,  YAMAGUCHI, Haruhiko ,  KURODA, Satoshi

IPC分类号： C12N15/82 ,  C12N9/10 ,  C07K14/415 ,  C12P5/00 ,  A01H5/00

CPC分类号： C12N15/8243 ,  C07K14/415 ,  C12N9/0006 ,  C12N9/1085 ,  C12N9/90 ,  C12N15/8209 ,  C12N15/8223 ,  C12P5/007 ,  C12Y101/01034 ,  C12Y205/0101 ,  C12Y205/0102 ,  C12Y205/01029 ,  C12Y503/03002

摘要： Provided is a vector capable of improving polyisoprenoid production through the introduction of the vector into a plant using gene recombination techniques. A vector comprising: a promoter of a gene encoding Small Rubber Particle Protein; and a gene encoding a protein involved in polyisoprenoid biosynthesis, the gene being functionally linked to the promoter.

摘要翻译： 本发明提供能够通过引入载体的成使用基因重组技术的植物改进聚类异戊二烯生产的载体。 一种载体，其包含：编码小橡胶颗粒的蛋白质的基因的启动子; 和编码在聚类异戊二烯生物合成相关的蛋白质的基因，该基因被功能性地连接于启动子。

公开(公告)号：EP2248902B1

公开(公告)日：2016-08-10

申请号：EP10008266.8

申请日：2003-02-20

申请人： J.R. Simplot Company

发明人： Rommens, Caius M. T. ,  Ye, Jingsong ,  Yan, Hua ,  Swords, Kathleen M. ,  Menendez-Humara, Jaime ,  Brinkerhoff, W. Leigh ,  Richael, Craig

IPC分类号： C12N15/82 ,  C12N9/10 ,  C12N9/12

CPC分类号： C12N15/8202 ,  A01H1/00 ,  A01H1/04 ,  A23L5/11 ,  A23L19/12 ,  A23L19/18 ,  C07K14/415 ,  C12N9/0059 ,  C12N9/1051 ,  C12N9/1294 ,  C12N15/8205 ,  C12N15/8209 ,  C12N15/821 ,  C12N15/8216 ,  C12N15/8218 ,  C12N15/8226 ,  C12N15/8243 ,  C12N15/8245 ,  C12N15/825 ,  C12N15/8273