公开(公告)号：EP0627080B1

公开(公告)日：2000-04-05

申请号：EP93905022.5

申请日：1993-02-11

申请人： COULTER CORPORATION

发明人： SHENKIN, Mark, L. ,  HAMELIK, Ronald, M. ,  WOOD, James, C., S.

IPC分类号： G01N33/48 ,  G01N33/50 ,  G01N33/577 ,  G01N33/58 ,  G01N15/14 ,  G01N1/30 ,  G01N33/569

CPC分类号： G01N33/5094 ,  G01N33/56972 ,  G01N2015/1477 ,  G01N2015/1486

摘要： A method is provided to ascertain the total number of cells per unit volume of a cell specimen in an apparatus which does not measure the volume of a suspension containing the specimen as it is being analyzed. The method comprising mixing a suspension of a known quantity of particles having a first light scatter signal with a known volume of a cell specimen having a second light scatter signal different from the first signal to obtain a suspension having a concentration of particles per specimen volume, passing each of said particles and each cell in said cell specimen, in turn, through a light beam, each of said particles and cells producing at least one forward light scattering pattern, counting the number of cells and the number of particles in a portion of said suspension, and determining the total number of cells per unit volume of specimen.

公开(公告)号：EP0752133A1

公开(公告)日：1997-01-08

申请号：EP94908640.0

申请日：1994-01-25

申请人： COULTER CORPORATION

发明人： AUER, Robert, E. ,  WEBER, Bruce, M. ,  STARLING, John, D. ,  WOOD, James, C., S.

IPC分类号： G01N21 ,  G01N15 ,  G01N33 ,  G06F17 ,  G06F19 ,  G01J1

CPC分类号： G01N15/14 ,  G01J2001/4406 ,  G01N15/1456

摘要： A flow cytometer (10) includes a plurality of detectors (28, 30, 32, 34, 36, 38) for providing voltage pulse signals over a four decade range as particles pass through an illuminated detection station (20). Each voltage pulse signal is processed and provided to a first sample and hold (84, 138) for storage. Thereafter, the value in the first sample and hold is amplified by one or thirty-two, depending upon its magnitude, and stored in a second sample and hold (84, 146) for subsequent provision to a sixteen bit analog to digital convertor (ADC) (66), one signal at a time. The first sample and hold is then free to store a new value while the old value is awaiting provision to the ADC. The ADC converts the signal received from the second sample and hold to a fifteen bit digital signal and uses the sixteenth bit to manifest whether the signal stored in the second sample and hold was amplified by thirty-two.

公开(公告)号：EP0752133B1

公开(公告)日：2000-06-28

申请号：EP94908640.9

申请日：1994-01-25

申请人： COULTER CORPORATION

发明人： AUER, Robert, E. ,  WEBER, Bruce, M. ,  STARLING, John, D. ,  WOOD, James, C., S.

IPC分类号： G01N15/14 ,  G06F17/40 ,  G06F19/00

CPC分类号： G01N15/14 ,  G01J2001/4406 ,  G01N15/1456

摘要： A flow cytometer (10) includes a plurality of detectors (28, 30, 32, 34, 36, 38) for providing voltage pulse signals over a four decade range as particles pass through an illuminated detection station (20). Each voltage pulse signal is processed and provided to a first sample and hold (84, 138) for storage. Thereafter, the value in the first sample and hold is amplified by one or thirty-two, depending upon its magnitude, and stored in a second sample and hold (84, 146) for subsequent provision to a sixteen bit analog to digital convertor (ADC) (66), one signal at a time. The first sample and hold is then free to store a new value while the old value is awaiting provision to the ADC. The ADC converts the signal received from the second sample and hold to a fifteen bit digital signal and uses the sixteenth bit to manifest whether the signal stored in the second sample and hold was amplified by thirty-two.

公开(公告)号：EP0627080A1

公开(公告)日：1994-12-07

申请号：EP93905022.0

申请日：1993-02-11

申请人： COULTER CORPORATION

发明人： SHENKIN, Mark, L. ,  HAMELIK, Ronald, M. ,  WOOD, James, C., S.

IPC分类号： G01N33 ,  G01N15

CPC分类号： G01N33/5094 ,  G01N33/56972 ,  G01N2015/1477 ,  G01N2015/1486

摘要： L'invention concerne un procédé qui permet d'évaluer le nombre total de cellules par unité de volume d'un échantillon contenant des cellules, dans un appareil qui ne mesure pas le volume d'une suspension contenant ledit échantillon pendant l'analyse. Ce procédé consiste à mélanger une suspension contenant une quantité connue de particules et qui présente un premier signal de dispersion de la lumière, avec un volume connu d'un échantillon de cellules présentant un deuxième signal de dispersion de la lumière qui diffère du premier, afin d'obtenir une suspension dotée d'une certaine concentration en particules par volume d'échantillon. Il consiste ensuite à faire passer successivement chacune desdites particules et desdites cellules dudit échantillon de cellules au travers d'un faisceau lumineux de sorte qu'elles produisent toutes au moins un schéma de dispersion de lumière vers l'avant. On compte alors le nombre de cellules et de particules présentes dans une partie de la suspension et on détermine le nombre total de cellules par unité de volume de l'échantillon.