公开(公告)号：EP2356246A1

公开(公告)日：2011-08-17

申请号：EP08875859.4

申请日：2008-11-17

申请人： CSIR

发明人： VISSER, Daniel, Finsch ,  GORDON, Gregory, Ernest, Robert ,  HENNESSY, Fritha ,  RASHAMUSE, Konanani, Justice ,  BRADY, Dean ,  BODE, Moira, Leanne ,  LOUW, Maureen, Elizabeth

IPC分类号： C12P19/38 ,  C12N9/10

CPC分类号： C12P19/385 ,  C12N9/1077

摘要： A method for manipulating a nucleoside includes converting guanosine to 5- methyluridine by means of biocatalytic transglycosylation. The biocatalyst comprises a combination of a purine nucleoside phosphorylase from Bacillus halodurans, PNPase (BH1531 ) of sequence ID No 1, or a protein with at least 90% sequence similarity thereto, and a pyhmidine nucleoside phosphorylase (PyNPase).

公开(公告)号：EP2356246B1

公开(公告)日：2012-08-22

申请号：EP08875859.4

申请日：2008-11-17

申请人： CSIR

发明人： VISSER, Daniel, Finsch ,  GORDON, Gregory, Ernest, Robert ,  HENNESSY, Fritha ,  RASHAMUSE, Konanani, Justice ,  BRADY, Dean ,  BODE, Moira, Leanne ,  LOUW, Maureen, Elizabeth

IPC分类号： C12P19/38 ,  C12N9/10

CPC分类号： C12P19/385 ,  C12N9/1077

摘要： A method for manipulating a nucleoside includes converting guanosine to 5- methyluridine by means of biocatalytic transglycosylation. The biocatalyst comprises a combination of a purine nucleoside phosphorylase from Bacillus halodurans, PNPase (BH1531 ) of sequence ID No 1, or a protein with at least 90% sequence similarity thereto, and a pyhmidine nucleoside phosphorylase (PyNPase).