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    METHODS FOR DETECTION OF NUCLEOTIDE MODIFICATION
    1.
    发明公开
    METHODS FOR DETECTION OF NUCLEOTIDE MODIFICATION 有权
    检测方法的NUKLEOTIDMODIFIZIERUNG的

    公开(公告)号：EP2737085A2

    公开(公告)日：2014-06-04

    申请号：EP12753553.2

    申请日：2012-07-27

    申请人： Cambridge Epigenetix Limited

    发明人： BOOTH, Michael, John ,  BALASUBRAMANIAN, Shankar

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6876 ,  C01G55/002 ,  C07H1/00 ,  C07H21/02 ,  C07H21/04 ,  C12Q1/6806 ,  C12Q1/6827 ,  C12Q2600/154 ,  C12Q2523/115 ,  C12Q2523/125

    摘要： This invention relates to the identification of modified cytosine residues, such as 5-methylcytosine (5mC), 5-hydroxymethylcytosine (5hmC) and 5-formylcytosine (5fC) to be distinguished from cytosine (C) in a sample nucleotide sequence. Methods may comprise oxidising or reducing a first portion of polynucleotides which comprise the sample nucleotide sequence; treating the oxidised or reduced first portion and a second portion of polynucleotides with bisulfite; sequencing the polynucleotides in the first and second portions of the population following steps ii) and iii) to produce first and second nucleotide sequences, respectively and; identifying the residue in the first and second nucleotide sequences which corresponds to a cytosine residue in the sample nucleotide sequence. These methods may be useful, for example in the analysis of genomic DNA and/or of RNA.