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1.发明授权METHOD TO MEASURE THE METABOLIC RATE OR RATE OF GLUCOSE CONSUMPTION OF CELLS OR TISSUES WITH HIGH SPATIOTEMPORAL RESOLUTION USING A GLUCOSE NANOSENSOR 有权使用葡萄糖纳米传感器测量具有高时间分辨率的细胞或组织的葡萄糖消耗的代谢率或速率的方法
公开(公告)号：EP2588854B1
公开(公告)日：2017-05-31
申请号：EP10854225.9
申请日：2010-06-30
IPC分类号： G01N33/574 , G01N33/00 , C12Q1/02 , C12Q1/54 , G01N33/66
CPC分类号： C12Q1/54 , C12Q1/025 , G01N33/574 , G01N33/66 , G01N2500/10
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2.发明公开METHOD TO MEASURE THE METABOLIC RATE OR RATE OF GLUCOSE CONSUMPTION OF CELLS OR TISSUES WITH HIGH SPATIOTEMPORAL RESOLUTION USING A GLUCOSE NANOSENSOR 有权法测量细胞的代谢或葡萄糖消耗速度或组织与高时间分辨率上国土葡萄糖传感器NANO的基础上的
公开(公告)号：EP2588854A1
公开(公告)日：2013-05-08
申请号：EP10854225.9
申请日：2010-06-30
IPC分类号： G01N33/00
CPC分类号： C12Q1/54 , C12Q1/025 , G01N33/574 , G01N33/66 , G01N2500/10
摘要： A method to measure the metabolic rate or rate of glucose consumption of cells or tissues with high spatiotemporal resolution using a glucose nanosensor is disclosed. The method generally includes providing single cells or cell populations, cells in suspension or adherent, in a cell culture, a tissue culture, a mixed cell culture, a tissue explant, or in animal tissues in vivo for the measurement of glucose metabolic rate; expressing a glucose sensor in individual cells; calibrating the sensor in controlled conditions; disrupting the steady-state of glucose entering the cell; recording the output from the sensor and calculating the corresponding glucose concentration at different times; and determining the glucose metabolic rate. The method can be applied for the screening of molecules with pharmacological potential, determination of glucose rate of cancerous cells, tissue physiology and biochemistry research.
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