公开(公告)号：EP1541690A2

公开(公告)日：2005-06-15

申请号：EP05002916.4

申请日：1999-09-09

申请人： CureVac GmbH

发明人： Hoerr, Ingmar ,  Rammensee, Hans-Georg, Prof. Dr. ,  Jung, Günther, Prof. Dr. ,  Obst, Reinhard, Dr.

IPC分类号： C12N15/87 ,  A61K38/00 ,  A61K48/00 ,  A61P31/12

CPC分类号： A61K9/0019 ,  A61K9/1272 ,  A61K48/00 ,  A61K2039/53 ,  C12N15/87 ,  Y02A50/412

摘要： The invention provides a method for the transfer of RNA, especially mRNA, into cells and into organisms. This method is especially suited for applications in the field of gene therapy. The RNA is transferred in the form of a complex between at least one RNA and at least one cationic, especially polycationic, peptide or protein. Preferably protamine, poly-L-lysine and/or histone is used as cationic peptide or protein.

摘要翻译： 本发明提供了将RNA特别是mRNA转移到细胞中并转化为生物体的方法。 该方法特别适用于基因治疗领域的应用。 RNA以至少一种RNA和至少一种阳离子，特别是阳离子，肽或蛋白质之间的复合物的形式转移。 优选使用鱼精蛋白，聚-L-赖氨酸和/或组蛋白作为阳离子肽或蛋白质。

公开(公告)号：EP1619254A1

公开(公告)日：2006-01-25

申请号：EP05022610.9

申请日：1999-09-09

申请人： CureVac GmbH

发明人： Hoerr, Ingmar ,  Rammensee, Hans-Georg, Prof. Dr. ,  Jung, Günther, Prof. Dr. ,  Obst, Reinhard, Dr.

IPC分类号： C12N15/87 ,  A61K38/00 ,  A61K48/00 ,  A61P31/12

CPC分类号： A61K9/0019 ,  A61K9/1272 ,  A61K48/00 ,  A61K2039/53 ,  C12N15/87 ,  Y02A50/412

摘要： The invention provides a method for the transfer of RNA, especially mRNA, into cells and into organisms. This method is especially suited for applications in the field of gene therapy. The RNA is transferred in the form of a complex between at least one RNA and at least one cationic, especially polycationic, peptide or protein. Preferably protamine, poly-L-lysine and/or histone is used as cationic peptide or protein.

公开(公告)号：EP1083232B1

公开(公告)日：2005-02-23

申请号：EP99117771.8

申请日：1999-09-09

申请人： CureVac GmbH

发明人： Jung, Günther, Prof.Dr. ,  Hoerr, Ingmar ,  Rammensee, Hans-Georg, Prof.Dr. ,  Obst, Reinhard, Dr.

IPC分类号： C12N15/87 ,  A61K48/00 ,  A61K38/00 ,  A61P31/12

CPC分类号： A61K9/0019 ,  A61K9/1272 ,  A61K48/00 ,  A61K2039/53 ,  C12N15/87 ,  Y02A50/412

摘要： The invention provides a method for the transfer of RNA, especially mRNA, into cells and into organisms. This method is especially suited for applications in the field of gene therapy. The RNA is transferred in the form of a complex between at least one RNA and at least one cationic, especially polycationic, peptide or protein. Preferably protamine, poly-L-lysine and/or histone is used as cationic peptide or protein.

公开(公告)号：EP1619254B1

公开(公告)日：2010-12-22

申请号：EP05022610.9

申请日：1999-09-09

申请人： CureVac GmbH

发明人： Hoerr, Ingmar, Dr. ,  Rammensee, Hans-Georg, Prof. Dr. ,  Jung, Günther, Prof. Dr. ,  Obst, Reinhard, Dr.

IPC分类号： C12N15/87 ,  A61K38/00 ,  A61K48/00 ,  A61P31/12

CPC分类号： A61K9/0019 ,  A61K9/1272 ,  A61K48/00 ,  A61K2039/53 ,  C12N15/87 ,  Y02A50/412

摘要： The invention provides a method for the transfer of RNA, especially mRNA, into cells and into organisms. This method is especially suited for applications in the field of gene therapy. The RNA is transferred in the form of a complex between at least one RNA and at least one cationic, especially polycationic, peptide or protein. Preferably protamine, poly-L-lysine and/or histone is used as cationic peptide or protein.

公开(公告)号：EP1818409A1

公开(公告)日：2007-08-15

申请号：EP07002200.9

申请日：1999-09-09

申请人： CureVac GmbH

发明人： Hoerr, Ingmar, Dr. ,  Rammensee, Hans-Georg, Prof. Dr. ,  Jung, Günther, Prof. Dr. ,  Obst, Reinhard, Dr.

IPC分类号： C12N15/87 ,  A61K38/00 ,  A61K48/00 ,  A61P31/12

CPC分类号： A61K9/0019 ,  A61K9/1272 ,  A61K48/00 ,  A61K2039/53 ,  C12N15/87 ,  Y02A50/412

摘要： The invention provides a method for the transfer of RNA, especially mRNA, into cells and into organisms. This method is especially suited for applications in the field of gene therapy. The RNA is transferred in the form of a complex between at least one RNA and at least one cationic, especially polycationic, peptide or protein. Preferably protamine, poly-L-lysine and/or histone is used as cationic peptide or protein.

公开(公告)号：EP1541690A3

公开(公告)日：2005-07-27

申请号：EP05002916.4

申请日：1999-09-09

申请人： CureVac GmbH

发明人： Hoerr, Ingmar ,  Rammensee, Hans-Georg, Prof. Dr. ,  Jung, Günther, Prof. Dr. ,  Obst, Reinhard, Dr.

IPC分类号： C12N15/87 ,  A61K38/00 ,  A61K48/00 ,  A61P31/12

CPC分类号： A61K9/0019 ,  A61K9/1272 ,  A61K48/00 ,  A61K2039/53 ,  C12N15/87 ,  Y02A50/412

摘要： The invention provides a method for the transfer of RNA, especially mRNA, into cells and into organisms. This method is especially suited for applications in the field of gene therapy. The RNA is transferred in the form of a complex between at least one RNA and at least one cationic, especially polycationic, peptide or protein. Preferably protamine, poly-L-lysine and/or histone is used as cationic peptide or protein.