公开(公告)号：EP1206493B1

公开(公告)日：2006-11-15

申请号：EP00953792.9

申请日：2000-08-02

申请人： DADE BEHRING INC.

发明人： KASPER, Kenneth, C. ,  JEONG, Henry ,  DAVALIAN, Dariush ,  LIU, Hshiou-Ting ,  MILLER, Paul, L. ,  WILLIAMS, Denise, L.

IPC分类号： C07K16/00

CPC分类号： A61K51/1093 ,  C07K16/1292 ,  Y10S436/815

摘要： An IgG1 lambda monoclonal antibody to the immunosuppressive drug tacrolimus has improved properties. In particular, this monoclonal antibody, designated 1H6, has reduced cross-reactivity to several tacrolimus metabolites. This antibody is suitable for performance of immunoassays such as homogeneous immunoassays to detect or determine the presence or concentration of tacrolimus in samples such as blood samples. The invention further includes derivatives of tacrolimus derivatized at a non-binding portion of the molecule useful in immunizing antibody-producing animals and in producing such monoclonal antibodies, as well as labeled derivatives of tacrolimus useful as tacrolimus analogues in such assays. The invention further includes immunoassay methods for the detection of tacrolimus and test kits useful in performing such immunoassays.

公开(公告)号：EP1206493A2

公开(公告)日：2002-05-22

申请号：EP00953792.9

申请日：2000-08-02

申请人： DADE BEHRING INC.

发明人： KASPER, Kenneth, C. ,  JEONG, Henry ,  DAVALIAN, Dariush ,  LIU, Hshiou-Ting ,  MILLER, Paul, L. ,  WILLIAMS, Denise, L.

IPC分类号： C07K16/00

CPC分类号： A61K51/1093 ,  C07K16/1292 ,  Y10S436/815

摘要： An IgG1μ monoclonal antibody to the immunosuppressive drug tacrolimus has improved properties. In particular, this monoclonal antibody, designated 1H6, has reduced cross-reactivity to several tacrolimus metabolites. This antibody is suitable for performance of immunoassays such as homogeneous immunoassays to detect or determine the presence or concentration of tacrolimus in samples such as blood samples. The invention further includes derivatives of tacrolimus derivatized at a non-binding portion of the molecule useful in immunizing antibody-producing animals and in producing such monoclonal antibodies, as well as labeled derivatives of tacrolimus useful as tacrolimus analogues in such assays. The invention further includes immunoassay methods for the detection of tacrolimus and test kits useful in performing such immunoassays.