公开(公告)号：EP1206493B1

公开(公告)日：2006-11-15

申请号：EP00953792.9

申请日：2000-08-02

申请人： DADE BEHRING INC.

发明人： KASPER, Kenneth, C. ,  JEONG, Henry ,  DAVALIAN, Dariush ,  LIU, Hshiou-Ting ,  MILLER, Paul, L. ,  WILLIAMS, Denise, L.

IPC分类号： C07K16/00

CPC分类号： A61K51/1093 ,  C07K16/1292 ,  Y10S436/815

摘要： An IgG1 lambda monoclonal antibody to the immunosuppressive drug tacrolimus has improved properties. In particular, this monoclonal antibody, designated 1H6, has reduced cross-reactivity to several tacrolimus metabolites. This antibody is suitable for performance of immunoassays such as homogeneous immunoassays to detect or determine the presence or concentration of tacrolimus in samples such as blood samples. The invention further includes derivatives of tacrolimus derivatized at a non-binding portion of the molecule useful in immunizing antibody-producing animals and in producing such monoclonal antibodies, as well as labeled derivatives of tacrolimus useful as tacrolimus analogues in such assays. The invention further includes immunoassay methods for the detection of tacrolimus and test kits useful in performing such immunoassays.

公开(公告)号：EP1206493A2

公开(公告)日：2002-05-22

申请号：EP00953792.9

申请日：2000-08-02

申请人： DADE BEHRING INC.

发明人： KASPER, Kenneth, C. ,  JEONG, Henry ,  DAVALIAN, Dariush ,  LIU, Hshiou-Ting ,  MILLER, Paul, L. ,  WILLIAMS, Denise, L.

IPC分类号： C07K16/00

CPC分类号： A61K51/1093 ,  C07K16/1292 ,  Y10S436/815

摘要： An IgG1μ monoclonal antibody to the immunosuppressive drug tacrolimus has improved properties. In particular, this monoclonal antibody, designated 1H6, has reduced cross-reactivity to several tacrolimus metabolites. This antibody is suitable for performance of immunoassays such as homogeneous immunoassays to detect or determine the presence or concentration of tacrolimus in samples such as blood samples. The invention further includes derivatives of tacrolimus derivatized at a non-binding portion of the molecule useful in immunizing antibody-producing animals and in producing such monoclonal antibodies, as well as labeled derivatives of tacrolimus useful as tacrolimus analogues in such assays. The invention further includes immunoassay methods for the detection of tacrolimus and test kits useful in performing such immunoassays.

公开(公告)号：EP1005650A1

公开(公告)日：2000-06-07

申请号：EP99923193.9

申请日：1999-05-19

申请人： DADE BEHRING INC.

发明人： PIRIO, Marcel, Rene ,  DAVALIAN, Dariush ,  ISHKANIAN, Jacqueline, Sadakan ,  ULLMAN, Edwin, F.

IPC分类号： G01N33/532 ,  G01N33/78 ,  G01N33/74

CPC分类号： G01N33/9453 ,  G01N33/532 ,  G01N33/743 ,  G01N33/78 ,  Y10S436/815 ,  Y10S436/817

摘要： The present invention relates to compounds that are bis-biotins. These compounds comprise two biotinyl radicals connected by a chain of atoms, usually at least 16 atoms in length. The bis-biotin is conjugated to a member of a specific binding pair ('sbp member') wherein the chain is not part of the sbp member. Also disclosed are compositions comprising a complex of avidin and a bis-biotin as described above. The compounds and compositions of the invention find use in an assay for an analyte wherein there is employed a reagent system comprising an avidin reagent and a biotin reagent. The improvement of the present invention comprises using as the biotin reagent a bis-biotin as described above. Also disclosed are kits comprising the present bis-biotins and methods of preparing a bis-biotinylated conjugate of a member of a specific binding pair ('sbp member') for use in a specific binding assay.