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公开(公告)号:EP0222412B1
公开(公告)日:1992-11-19
申请号:EP86115833.5
申请日:1986-11-14
发明人: Maeda, Susumu , Furusawa, Mitsuru , Marumoto, Yasumasa , Horiuchi, Tadashi , Sato, Yoshinari , Saeki, Yoshiyuki
CPC分类号: C12N15/86 , C07K14/005 , C07K14/56 , C07K14/65 , C07K2319/00 , C07K2319/09 , C07K2319/735 , C07K2319/75 , C12N2710/14122 , C12N2710/14143
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公开(公告)号:EP0222412A2
公开(公告)日:1987-05-20
申请号:EP86115833.5
申请日:1986-11-14
发明人: Maeda, Susumu , Furusawa, Mitsuru , Marumoto, Yasumasa , Horiuchi, Tadashi , Sato, Yoshinari , Saeki, Yoshiyuki
CPC分类号: C12N15/86 , C07K14/005 , C07K14/56 , C07K14/65 , C07K2319/00 , C07K2319/09 , C07K2319/735 , C07K2319/75 , C12N2710/14122 , C12N2710/14143
摘要: Recombinant Bombyx mon nuclear polyhedrosis viruses having, in the polyhedral protein-encoding structural gene portion of the Bombyx mon nuclear polyhedrosis virus DNA (BmNPV DNA), a structural gene for a desired protein as joined to the whole of or a part of the polyhedral protein-encoding structural gene with or without interposition of a linker base sequence are provided.
By propagating the above viruses in silkworm-derived cells or silkworms, fused proteins of a desired protein and the whole of or a part of the polyhedral protein as joined together with or without interposition of a linking amino acid or peptide are produced. The desired proteins can be obtained from the fused proteins by cleavage or decomposition at the linking site.摘要翻译: 在家蚕核型多角体病毒DNA(BmNPV DNA)的多面体蛋白质编码结构基因部分中具有与多面体蛋白质的全部或部分连接的所需蛋白质的结构基因的重组家蚕核型多角体病毒 提供了具有或没有插入连接体碱基序列的编码结构基因。 通过在蚕衍生的细胞或家蚕中繁殖上述病毒,产生所需蛋白质的融合蛋白质和多面体蛋白质的全部或部分在连接或不插入连接氨基酸或肽的情况下连接在一起。 可以通过在连接位点的切割或分解从融合的蛋白质获得所需的蛋白质。
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