公开(公告)号：EP2928290B1

公开(公告)日：2017-05-31

申请号：EP13811407.9

申请日：2013-12-06

申请人： Alternative Gene Expression, S.L.

发明人： GOMEZ SEBASTIAN, Silvia ,  LOPEZ VIDAL, Javier ,  MARTINEZ ESCRIBANO, José Angel

IPC分类号： C12N15/86 ,  C12N7/00 ,  A01K67/033 ,  C12N15/85 ,  C07K14/005

CPC分类号： C12N15/86 ,  C07H21/04 ,  C07K14/005 ,  C12N7/00 ,  C12N15/85 ,  C12N2502/00 ,  C12N2510/00 ,  C12N2710/14022 ,  C12N2710/14023 ,  C12N2710/14034 ,  C12N2710/14041 ,  C12N2710/14122 ,  C12N2710/14131 ,  C12N2710/14143 ,  C12N2710/20022 ,  C12N2710/20034 ,  C12N2710/20051 ,  C12N2750/10022 ,  C12N2750/10034 ,  C12N2750/10051 ,  C12N2770/16022 ,  C12N2770/16034 ,  C12N2770/16051

摘要： The present invention may be included in the field of biotechnology and it covers the improved production of recombinant proteins in insect cells or insect larvae as biofactories by a novel expression cassette. This expression cassette comprises nucleic acid sequences such as promoters, homologous regions ( hr ) as enhancers, and sequences encoding transcriptional regulators, for example, the baculovirus Ac-ie-01 cDNA, or any combination thereof, which are able to increase the quality and production efficiency of the recombinant proteins. Moreover, the present invention is also directed to the vectors themselves comprising the above mentioned nucleic acid sequences of the invention, cells or insects infected, transformed or transfected with those sequences or vectors, and methods for producing the recombinant proteins by using the aforesaid sequences, vectors, cells or insects.

摘要翻译： 本发明可以包括在生物技术领域中，其涵盖了通过新颖的表达盒在昆虫细胞或昆虫幼虫中作为生物物质改进的重组蛋白的生产。 该表达盒包括核酸序列，例如启动子，作为增强子的同源区（hr）和编码转录调节子的序列，例如杆状病毒Ac-ie-01 cDNA或其任何组合，其能够提高质量和 重组蛋白的生产效率。 此外，本发明还涉及包含本发明的上述核酸序列的载体本身，用这些序列或载体感染，转化或转染的细胞或昆虫，以及通过使用上述序列生产重组蛋白的方法， 载体，细胞或昆虫。

公开(公告)号：EP1514928A4

公开(公告)日：2009-08-05

申请号：EP03733287

申请日：2003-06-04

申请人： CHUGAI PHARMACEUTICAL CO LTD

发明人： KODAMA TATSUHIKO ,  JISHAGE KOU-ICHI ,  KAMADA NOBUO ,  YAMADA YOSHIKI

IPC分类号： A01K67/027 ,  A61K38/11 ,  C07K14/01 ,  C07K16/00 ,  C07K16/18 ,  C07K16/30 ,  C12N15/85 ,  C12N15/09 ,  C07K16/08 ,  C12N5/16

CPC分类号： C12N15/8509 ,  A01K67/0275 ,  A01K2217/05 ,  A01K2227/105 ,  A01K2267/01 ,  A01K2267/02 ,  A61K38/00 ,  A61K2039/505 ,  A61K2039/5256 ,  C07K14/005 ,  C07K16/00 ,  C07K16/18 ,  C07K16/3076 ,  C12N2710/14122

摘要： A method of producing an antibody which involves the step of administering an immunogen containing a target antigen and a background antigen to a transgenic animal having a gene encoding the background antigen transferred thereinto. Because of being immunotolerant to the background antigen, the transgenic animal effectively produces an antibody against the target antigen.

公开(公告)号：EP1637604A2

公开(公告)日：2006-03-22

申请号：EP05021610.0

申请日：2002-03-05

申请人： Fujirebio Inc.

发明人： Inaba, Niro, c/o Fujirebio Inc. ,  Hori, Takeya, c/o Fujirebio Inc. ,  Ito, Satoru, c/o Fujirebio Inc.

IPC分类号： C12N15/62 ,  C12P21/02 ,  C12P21/06 ,  C07K14/715 ,  C07K14/01 ,  C12N15/34 ,  C12N15/866 ,  C12N9/10 ,  C12N15/54

CPC分类号： C12N9/1048 ,  C07K14/005 ,  C07K14/7158 ,  C07K2319/00 ,  C07K2319/03 ,  C12N7/00 ,  C12N15/62 ,  C12N15/86 ,  C12N2710/14022 ,  C12N2710/14122 ,  C12N2710/14143 ,  C12P21/02

摘要： A method for producing a desired protein by genetic engineering process by which the desired protein can easily be recovered without denaturation is disclosed. In this method, the desired protein is produced in the form a fusion protein with a protein constituting a virus particle, and the virus particle is recovered. Since the virus particles are larger than usual protein molecules occurring in the cells, the particles can be easily recovered by centrifugation or the like.

摘要翻译： 公开了通过遗传工程方法生产所需蛋白质的方法，通过该方法可以容易地在不变性的情况下回收所需的蛋白质。 在该方法中，以构成病毒颗粒的蛋白质的融合蛋白的形式生成所需的蛋白质，回收病毒粒子。 由于病毒颗粒大于细胞中发生的通常的蛋白质分子，因此可以通过离心等容易地回收颗粒。

公开(公告)号：EP0336341B1

公开(公告)日：1994-06-22

申请号：EP89105818.2

申请日：1989-04-03

申请人： BOYCE THOMPSON INSTITUTE FOR PLANT RESEARCH

发明人： Granados, Robert R.

IPC分类号： C07K15/04 ,  A01N63/04

CPC分类号： C07K14/005 ,  A01N63/00 ,  A01N63/02 ,  C12N2710/14122

公开(公告)号：EP0336341A3

公开(公告)日：1990-10-31

申请号：EP89105818.2

申请日：1989-04-03

申请人： BOYCE THOMPSON INSTITUTE FOR PLANT RESEARCH

发明人： Granados, Robert R.

IPC分类号： C07K15/04 ,  A01N63/04

CPC分类号： C07K14/005 ,  A01N63/00 ,  A01N63/02 ,  C12N2710/14122

摘要： Nuclear polyhedrosis viruses, for example, Autographa californica nuclear polyhedrosis virus (AcMNPV), use­ful in the control of lepidopterous larvae such as the larvae of the cabbage looper Trichoplusia ni , have been found to have enhanced infectivity when mixed with certain proteins obtained from the gra­nulin fraction of Trichoplusia ni granulosis virus (TnGV) or Heliothis armigera granulosis virus (HaGV), and from the polyhedrin fraction of AcMNPV viruses. The proteins from the TnGV granulin fraction have molecular weights between 101 and 104 Kd. The enhanced infectivity is correlated to biochemical and structural changes in the T.ni peritrophic mem­brane.

公开(公告)号：EP0384294A2

公开(公告)日：1990-08-29

申请号：EP90102939.7

申请日：1990-02-15

申请人： BOYCE THOMPSON INSTITUTE FOR PLANT RESEARCH, INC.

发明人： Granados, Robert R. ,  Hashimoto, Yoshifumi, Dept. of Applied Biology

IPC分类号： C12N15/34 ,  A01N63/00 ,  C07K15/04

CPC分类号： C12N15/8286 ,  A01N63/02 ,  C07K14/005 ,  C12N2710/14122 ,  Y02A40/162

摘要： A gene coded for a polypeptide isolated from an occlusion body of a baculovirus such as Trichoplusia ni granulosis virus possessing a biological activity of enhancing virus infection of host insects by causing rapid degradation of the peritrophic membrane lining the midgut lumen of insects. Novel pesticides incorporating said gene or fragments thereof are also claimed.

摘要翻译： 编码用于分离自杆状病毒（例如粉纹夜蛾）病毒的杆状病毒的闭塞体的多肽的基因，具有通过引起昆虫中肠内脏的内皮细胞膜的快速降解来增强宿主昆虫的病毒感染的生物学活性。 还要求引用所述基因或其片段的新型农药。

公开(公告)号：EP0336341A2

公开(公告)日：1989-10-11

申请号：EP89105818.2

申请日：1989-04-03

申请人： BOYCE THOMPSON INSTITUTE FOR PLANT RESEARCH

发明人： Granados, Robert R.

IPC分类号： C07K15/04 ,  A01N63/04

CPC分类号： C07K14/005 ,  A01N63/00 ,  A01N63/02 ,  C12N2710/14122

摘要： Nuclear polyhedrosis viruses, for example, Autographa californica nuclear polyhedrosis virus (AcMNPV), use­ful in the control of lepidopterous larvae such as the larvae of the cabbage looper Trichoplusia ni , have been found to have enhanced infectivity when mixed with certain proteins obtained from the gra­nulin fraction of Trichoplusia ni granulosis virus (TnGV) or Heliothis armigera granulosis virus (HaGV), and from the polyhedrin fraction of AcMNPV viruses. The proteins from the TnGV granulin fraction have molecular weights between 101 and 104 Kd. The enhanced infectivity is correlated to biochemical and structural changes in the T.ni peritrophic mem­brane.

摘要翻译： 已经发现用于控制鳞翅目幼虫如卷心菜叶卷毛虱（Trichoplusia ni）幼虫的核型多角体病毒例如苜蓿银纹夜蛾核型多角体病毒（AcMNPV）与从颗粒蛋白得到的某些蛋白质混合时具有增强的感染性 粉纹夜蛾丝粒病毒（TnGV）或黑喉甲壳纲粒细胞病毒（HaGV）的部分，以及来自AcMNPV病毒的多角体蛋白部分。 来自TnGV颗粒蛋白部分的蛋白质具有101至104Kd的分子量。 增强的感染性与T.ni peritrophic膜的生化和结构变化相关。

公开(公告)号：EP0222412A3

公开(公告)日：1987-09-09

申请号：EP86115833

申请日：1986-11-14

申请人： DAIICHI SEIYAKU CO. LTD.

发明人： Maeda, Susumu ,  Furusawa, Mitsuru ,  Marumoto, Yasumasa ,  Horiuchi, Tadashi ,  Sato, Yoshinari ,  Saeki, Yoshiyuki

IPC分类号： C12N15/00 ,  C12N07/00 ,  C12N07/02 ,  C12P21/02

CPC分类号： C12N15/86 ,  C07K14/005 ,  C07K14/56 ,  C07K14/65 ,  C07K2319/00 ,  C07K2319/09 ,  C07K2319/735 ,  C07K2319/75 ,  C12N2710/14122 ,  C12N2710/14143

摘要： Recombinant Bombyx mon nuclear polyhedrosis viruses having, in the polyhedral protein-encoding structural gene portion of the Bombyx mon nuclear polyhedrosis virus DNA (BmNPV DNA), a structural gene for a desired protein as joined to the whole of or a part of the polyhedral protein-encoding structural gene with or without interposition of a linker base sequence are provided. By propagating the above viruses in silkworm-derived cells or silkworms, fused proteins of a desired protein and the whole of or a part of the polyhedral protein as joined together with or without interposition of a linking amino acid or peptide are produced. The desired proteins can be obtained from the fused proteins by cleavage or decomposition at the linking site.

公开(公告)号：EP3294329A1

公开(公告)日：2018-03-21

申请号：EP16797022

申请日：2016-05-13

申请人： REBER GENETICS CO LTD

发明人： CHANG CHIA JUNG ,  LIAO YAN CHIOU ,  CHOU WEI I ,  CHANG HSIU KANG

IPC分类号： A61K39/04 ,  C07K14/005 ,  C12N15/86 ,  C12N15/866

CPC分类号： A61K39/12 ,  C07K14/005 ,  C07K2319/02 ,  C07K2319/735 ,  C12N2710/14122 ,  C12N2710/14143 ,  C12N2770/20022 ,  C12N2770/20034 ,  C12N2770/24322 ,  C12N2770/24334

摘要： A recombinant baculovirus displaying on its envelop a fusion protein is disclosed. The fusion protein comprises a heterologous antigen, and a C-terminal region of baculovirus envelope GP64 protein, which has at least 100 amino acid residues in length and lacks a B12D5 binding epitope located within the central basic region of the GP64 protein. The genome of the recombinant baculovirus comprises a transgene encoding a fusion protein that comprises a signal peptide, the heterologous antigen, and the C-terminal region of the baculovirus envelope GP64 protein, in which the antigen is located between the signal peptide and the C-terminal region of the GP64 protein. Methods for eliciting an antigen-specific immunogenic response in a subject in need thereof are also disclosed.

公开(公告)号：EP2191002B1

公开(公告)日：2016-04-13

申请号：EP08792433.8

申请日：2008-08-06

申请人： Educational Foundation Jichi Medical University ,  Otsuka Pharmaceutical Co., Ltd.

发明人： YOSHIDA, Shigeto ,  KAWASAKI, Masanori ,  MATSUMOTO, Makoto ,  OHBA, Yoshio ,  SAITO, Masahiro ,  GOTO, Yoshihiro ,  INAGAKI, Katsuya ,  MIZUKOSHI, Masami ,  HARIGUCHI, Norimitsu ,  HIROTA, Kuniko

IPC分类号： C12N15/866 ,  A61K39/145 ,  A61K39/015

CPC分类号： C07K16/1018 ,  A61K39/015 ,  A61K39/12 ,  A61K39/145 ,  A61K2039/5256 ,  C07K14/005 ,  C07K14/445 ,  C07K16/205 ,  C07K2319/735 ,  C12N7/00 ,  C12N15/86 ,  C12N2710/14122 ,  C12N2710/14143 ,  C12N2760/16122 ,  C12N2760/16134 ,  C12N2830/15 ,  C12N2830/60 ,  C12N2830/85 ,  Y02A50/412

摘要： The present invention provides a novel transfer vector and a recombinant baculovirus; methods for the production thereof; and pharmaceuticals containing the recombinant baculovirus as an active ingredient, which are useful as preventive or therapeutic drugs for infectious diseases such as malaria and influenza. More specifically, the invention provides a recombinant transfer vector capable of expressing a foreign gene fused to a virus gene under the control of a dual promoter; a recombinant baculovirus; methods for the production thereof; and pharmaceuticals containing the recombinant baculovirus as an active ingredient.