公开(公告)号：EP1832654A1

公开(公告)日：2007-09-12

申请号：EP05822796.8

申请日：2005-12-27

申请人： DAIICHI PURE CHEMICALS CO., LTD.

发明人： MATSUO, Masanao, Daiichi Pure Chemicals Co., Ltd. ,  EBINUMA, Hiroyuki, Daiichi Pure Chemicals Co., Ltd. ,  MIYAZAKI, Osamu, Daiichi Pure Chemicals Co., Ltd. ,  TANAKA, Kyoko, Daiichi Pure Chemicals Co., Ltd. ,  SUZUKI, Akiko, Daiichi Pure Chems. Co., Ltd.

IPC分类号： C12N15/02 ,  C07K16/36 ,  C12N5/10 ,  G01N33/53 ,  G01N33/577 ,  C12P21/08

CPC分类号： C07K16/18 ,  C07K14/745 ,  C07K14/75 ,  G01N33/86 ,  G01N2333/75 ,  G01N2800/224

摘要： The present invention is directed to a monoclonal antibody against a soluble fibrin, which specifically recognizes a conformation-changed site newly occurred in a C-terminal region of an Aα-chain of the soluble fibrin formed through thrombin digestion of fibrinogen. The present invention is also directed to a hybridoma which produces the antibody, an immunological assay method employing the antibody, and a method for evaluating hypercoagulability in a test sample by measuring the soluble fibrin level in the sample with the assay method. Through employment of the monoclonal antibody of the present invention, soluble fibrin on which plasmin has not acted, which reflects exclusively initial hypercoagulability, can be specifically detected.

摘要翻译： 本发明涉及针对可溶性血纤蛋白，其特异性识别的构象改变的位点新发生在A±通过纤维蛋白原的凝血酶消化所形成的可溶性纤维蛋白的 - 链式的C-末端区的单克隆抗体。 因此，本发明涉及一种杂交瘤产生的抗体，以免疫学测定方法用人抗体，并用于通过测量与测定方法在样品中的可溶性纤维蛋白水平的测试样品中评价高凝状态的方法。 通过采用本发明的单克隆抗体的，在其上纤溶酶可溶性纤维蛋白没有采取行动，这反映独占初始高凝状态，可以特异性地检测。