公开(公告)号：EP3045915A4

公开(公告)日：2017-07-26

申请号：EP14844963

申请日：2014-09-10

申请人： DENKA SEIKEN KK

发明人： INANO KOICHI ,  MIYAZAWA TAKASHI ,  ISHIKAWA OSAMU

IPC分类号： G01N33/569 ,  C07K16/10 ,  G01N33/558

CPC分类号： G01N33/56983 ,  C07K16/1018 ,  C07K2317/14 ,  C07K2317/34 ,  G01N33/558 ,  G01N33/577 ,  G01N2333/11 ,  G01N2469/10

摘要： A method for measuring influenza A virus by an immunoassay using an anti-influenza A virus monoclonal antibody which is highly reactive with a wide range of subtypes is disclosed. The method for measuring influenza A virus includes measuring influenza A virus by an immunoassay utilizing antigen-antibody reaction between a monoclonal antibody which specifically reacts with matrix protein (M1) of influenza A virus, or an antigen-binding fragment thereof, and influenza A virus in a sample.

摘要翻译： 公开了使用对广泛亚型具有高度反应性的抗甲型流感病毒单克隆抗体通过免疫测定来测量甲型流感病毒的方法。 测量甲型流感病毒的方法包括通过利用与甲型流感病毒的基质蛋白（M1）或其抗原结合片段特异性反应的单克隆抗体与甲型流感病毒之间的抗原 - 抗体反应的免疫测定来测量甲型流感病毒 在一个样本中。

公开(公告)号：EP3045914A4

公开(公告)日：2017-04-12

申请号：EP14843998

申请日：2014-09-10

申请人： DENKA SEIKEN KK

发明人： INANO KOICHI ,  MIYAZAWA TAKASHI ,  ISHIKAWA OSAMU

IPC分类号： G01N33/569 ,  C07K16/10 ,  C12P21/08 ,  G01N33/558

CPC分类号： G01N33/56983 ,  C07K16/1018 ,  G01N33/558 ,  G01N33/577 ,  G01N2333/11 ,  G01N2469/10

摘要： A method for measuring influenza B virus by an immunoassay, which method enables specific detection of influenza B virus with a higher sensitivity than conventional methods, and a device or a kit therefor are disclosed. The method for measuring influenza B virus includes carrying out an immunoassay of influenza B virus by a sandwich method using two kinds of monoclonal antibodies each of which specifically reacts with the region of the 125th to 248th amino acids of matrix protein (M1) of influenza B virus, which two kinds of monoclonal antibodies are capable of binding to the region of the 125th to 248th amino acids of M1 at the same time, or antigen-binding fragments thereof.

公开(公告)号：EP3029462A4

公开(公告)日：2017-03-22

申请号：EP14831189

申请日：2014-07-31

申请人： DENKA SEIKEN KK

发明人： KOHIYAMA RISA ,  ISHIKAWA OSAMU ,  MIYAZAWA TAKASHI

IPC分类号： G01N33/543 ,  G01N21/64 ,  G01N21/78 ,  G01N33/558

CPC分类号： G01N33/54313 ,  B01D15/3809 ,  G01N21/6428 ,  G01N33/545 ,  G01N33/558 ,  G01N2021/6439 ,  G01N2201/0636

摘要： The present invention intends to provide an immunochromatographic test piece that makes it possible to achieve both highly sensitive detection of a substance to be detected and a simple test piece structure, which are usually difficult to be made compatible with each other. The immunochromatographic test piece is an immunochromatographic test piece comprising a membrane on which a capture substance being a ligand that bonds to a substance to be detected is immobilized, wherein insoluble carrier particles to which a ligand that bonds to the substance to be detected is bound are used and accumulated by being captured with the capture substance immobilized on the membrane, the membrane is irradiated with light to detect light emitted from a portion where the insoluble carrier particles are accumulated or light emitted from a portion surrounding and other than the portion where the insoluble carrier particles are accumulated, thereby measuring the substance to be detected, and a light-reflecting material is provided on a side of the membrane opposite to a side irradiated with light.