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1.发明公开METHOD OF QUANTIFYING CHOLESTEROL IN HIGH DENSITY LIPOPROTEIN AND REAGENT COMPOSITIONS 有权方法胆固醇在HD（高密度）脂蛋白及试剂数量化
公开(公告)号：EP1477570A4
公开(公告)日：2006-03-29
申请号：EP03734880
申请日：2003-01-30
申请人： DENKA SEIKEN KK
发明人： MATSUI HIROSHI , OHTA MOTOKO
CPC分类号： C12Q1/60
摘要： A method comprising the first step of eliminating cholesterols in lipoproteins other than HDL and the second step of specifically quantifying HDL cholesterol, whereby accurate data can be obtained even in the case of special samples concerning lipid metabolic disorders, lipoprotein disorders, etc. This method of quantifying cholesterol in high density lipoproteins comprises the first wherein a test sample is treated with cholesterol esterase and cholesterol oxidase in the absence of any surfactant acting on high density lipoproteins and the thus formed hydrogen peroxide is removed to thereby eliminate cholesterol in lipoproteins other than high density lipoproteins, and the second step wherein a surfactant acting specifically on high density lipoproteins is added to the product obtained in the step 1 and then cholesterol in the high density lipoproteins is quantified based on hydrogen peroxide formed by the actions of the cholesterol esterase and the cholesterol oxidase. As the cholesterol oxidase in the step 1, use is made of one having a molecular weight of 60 kD or less.
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2.发明公开METHOD FOR REMOVAL OF TRIGLYCERIDES IN LIPOPROTEINS OTHER THAN LOW-DENSITY LIPOPROTEINS 审中-公开PROCESS FOR LDL以外的脂蛋白中的甘油三酯拆除
公开(公告)号：EP2840145A4
公开(公告)日：2015-12-23
申请号：EP13777616
申请日：2013-04-19
申请人： DENKA SEIKEN KK
发明人： OHTA MOTOKO
CPC分类号： C07K1/14 , C12Q1/26 , C12Q1/44 , C12Q1/485 , C12Q1/61 , G01N33/6893 , G01N33/92 , G01N2800/32 , G01N2800/7085
摘要： Disclosed is a method for selectively eliminating triglycerides in lipoproteins other than low density lipoprotein, which method allows one to provide a method for directly and differentially quantifying LDL-TG in a sample with excellent simplicity, specificity and accuracy using an automated analyzer or the like without performing a laborious operation of pretreatment such as centrifugation or electrophoresis. The method for eliminating triglycerides in lipoproteins other than low density lipoproteins includes allowing lipoprotein lipase, cholesterol esterase, glycerol kinase and glycerol-3-phosphate oxidase to act on a sample in the presence of a surfactant that acts on lipoproteins other than low density lipoprotein and/or a surfactant having LDL-protecting action, and eliminating hydrogen peroxide produced thereby.
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