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    DATA INDEPENDENT ACQUISITION OF PRODUCTION SPECTRA AND REFERENCE SPECTRA LIBRARY MATCHING
    1.
    发明公开
    DATA INDEPENDENT ACQUISITION OF PRODUCTION SPECTRA AND REFERENCE SPECTRA LIBRARY MATCHING 审中-公开
    日本麻省理工学院埃里克森·皮恩·伯恩

    公开(公告)号：EP2617052A2

    公开(公告)日：2013-07-24

    申请号：EP11778958.6

    申请日：2011-09-14

    申请人： DH Technologies Development Pte. Ltd. ,  ETH Zurich

    发明人： BONNER, Ronald ,  TATE, Stephen ,  AEBERSOLD, Rudolf ,  NAVARRO ALVAREZ, Pedro Jose ,  RINNER, Oliver ,  REITER, Lukas ,  GILLET, Ludovic

    IPC分类号： H01J49/00

    CPC分类号： H01J49/0036 ,  G01N30/7233 ,  G06F19/703 ,  G06F19/709 ,  H01J49/0031 ,  H01J49/004 ,  H01J49/0045 ,  H01J49/42

    摘要： Systems and methods are used to store an electronic record of all product ion spectra of all detectable compounds of a sample. A plurality of product ion scans are performed on a tandem mass spectrometer one or more times in a single sample analysis across a mass range using a plurality of mass selection windows. All sample product ion spectra of all detectable compounds for each mass selection window are produced. All sample product ion spectra for each mass selection window are received from the tandem mass spectrometer using a processor. All sample product ion spectra for each mass selection window are stored as an electronic record of all detectable compounds of the sample using the processor. The electronic record is used to characterize compounds known at the time the electronic record is stored or to characterize compounds that became known after the electronic record was stored.

    摘要翻译： 公开了用于使用重叠的前体隔离窗分析样品的系统和方法。 指示串联质谱仪的质量分析器使用处理器在样品的前体离子质量范围内选择和分割至少两个重叠的前体隔离窗口。 串联质谱仪包括质量分析器，其允许在样品质量范围内重叠的前体隔离窗口。

    METHOD FOR HIGH THROUGHPUT PEPTIDE/PROTEIN ASSAY GENERATION AND ASSAYS GENERATED THEREWITH
    3.
    发明公开
    METHOD FOR HIGH THROUGHPUT PEPTIDE/PROTEIN ASSAY GENERATION AND ASSAYS GENERATED THEREWITH 有权
    具有高吞吐量肽/蛋白质的含量测定生产方法和这样产生ASSAYS

    公开(公告)号：EP2283366A1

    公开(公告)日：2011-02-16

    申请号：EP09749631.9

    申请日：2009-05-20

    申请人： ETH Zurich

    发明人： AEBERSOLD, Ruedi ,  PICOTTI, Paola ,  RINNER, Oliver ,  MALMSTROEM, Johan

    IPC分类号： G01N33/68

    CPC分类号： G01N33/6848

    摘要： The invention relates to a method for the determination of an MRM or SRM assay for a protein of interest, a peptide of interest, or a group of proteins/peptides of interest or a whole proteome. It essentially includes the following steps: (1) a list of proteins of interest is selected and for each member at least one or a list of candidate proteotypic peptides is derived (2) this at least one peptide is synthesized/generated essentially without subsequent purification; (3) this at least one unpurified peptide is analyzed by selected reaction monitoring (SRM) preferably coupled to liquid chromatography (LC-SRM) or analogous techniques; (4) validation and/or optimisation of the corresponding assay of the at least one peptide with determination of the SRM coordinates for a peptide/protein of interest and/or of a regulator of interest is achieved. A protein sample of interest is enzymatically digested and can then be analyzed in SRM mode or time-constrained SRM mode, using elution times to trigger acquisition of the set of selected SRM traces, thus drastically increasing the throughput. The analysis allows to detect and quantify the set of peptides/proteins of interest. The method additionally relates to a tagging strategy to achieve absolute quantification of the peptides/proteins of interest at low-budget and high-throughput.