公开(公告)号：EP1717317A4

公开(公告)日：2009-02-11

申请号：EP05703933

申请日：2005-01-20

申请人： DNAVEC RESEARCH INC

发明人： IIDA AKIHIRO ,  BAN HIROSHI ,  INOUE MAKOTO ,  HIRATA TAKAHIRO ,  HASEGAWA MAMORU

IPC分类号： C12N15/86 ,  C12N5/10

CPC分类号： C12N15/86 ,  C12N2770/36143 ,  C12N2800/108 ,  C12N2800/30 ,  C12N2830/00 ,  C12N2830/15 ,  C12N2830/42 ,  C12N2830/90

摘要： It is intended to provide a method of producing a minus strand RNA virus vector characterized in that the transcription of the genomic RNA of the minus strand RNA virus vector and the expression of a minus strand RNA virus protein forming a ribonucleoprotein together with the genomic RNA are induced by a promoter containing a cytomegalovirus enhancer and an avian β-actin promoter. According to this method, a minus strand RNA virus vector having a high safety can be produced at a high efficiency. This method is particularly useful in producing a minus strand RNA virus vector lacking an envelope-constituting protein gene.

公开(公告)号：EP1950307A4

公开(公告)日：2010-09-08

申请号：EP06822478

申请日：2006-10-27

申请人： DNAVEC RESEARCH INC

发明人： MITOMO KATSUYUKI ,  INOUE MAKOTO ,  IWASAKI HITOSHI ,  HASEGAWA MAMORU

IPC分类号： C12N15/867 ,  A61K35/76 ,  A61K38/00 ,  A61K48/00 ,  A61P11/00 ,  C12N5/10

CPC分类号： C12N15/86 ,  A61K9/0043 ,  A61K38/162 ,  A61K38/1709 ,  A61K38/177 ,  A61K48/00 ,  C07K14/4712 ,  C12N5/0688 ,  C12N7/00 ,  C12N2510/00 ,  C12N2740/15033 ,  C12N2740/15043 ,  C12N2740/15045 ,  C12N2810/6072 ,  C12N2810/609

摘要： The present inventors successfully introduced genes into stem cells of airway epithelial tissues using simian immunodeficiency virus vectors pseudotyped with F and HN, which are envelope glycoproteins of Sendai virus. Gene transfer into airway epithelial tissue stem cells using a vector of the present invention is useful for gene therapy of genetic respiratory diseases such as cystic fibrosis. Furthermore, it is possible to select respiratory organs such as the lungs as production tissues for providing proteins that are deficient due to genetic diseases.

公开(公告)号：EP1712243A4

公开(公告)日：2007-04-11

申请号：EP05703477

申请日：2005-01-12

申请人： DNAVEC RESEARCH INC

发明人： IWADATE YASUO ,  YAMAURA AKIRA ,  INOUE MAKOTO ,  HASEGAWA MAMORU

IPC分类号： A61K48/00 ,  A61K35/12 ,  A61K35/76 ,  A61K38/00 ,  A61K39/00 ,  A61P35/00 ,  C07K14/47 ,  C07K14/55 ,  C12N5/10 ,  C12N15/09

CPC分类号： C07K14/55 ,  A61K38/00 ,  A61K39/0011 ,  A61K2039/53 ,  A61K2039/55533 ,  C07K14/4748 ,  C12N2760/18832 ,  C12N2760/18843

摘要： The present invention provides methods for treating tumors, which comprise the step of administering into tumor sites a minus-strand RNA viral vector encoding an immunostimulatory cytokine or cells introduced with the vector. The present invention also provides compositions for treating tumors, which comprise as an active ingredient the minus-strand RNA viral vector encoding an immunostimulatory cytokine or cells introduced with the vector. The present invention also provides kits for treating tumors, which comprise the minus-strand RNA viral vector encoding an immunostimulatory cytokine, and a tumor antigen or a vector expressing the antigen.

公开(公告)号：EP1505154A4

公开(公告)日：2006-09-06

申请号：EP03728016

申请日：2003-04-30

申请人： DNAVEC RESEARCH INC

发明人： KINOH HIROAKI ,  INOUE MAKOTO ,  UEDA YASUJI ,  IIDA AKIHIRO ,  HASEGAWA MAMORU ,  KOBAYASHI MASANORI

IPC分类号： A61K38/00 ,  A61K48/00 ,  A61P35/00 ,  C07K14/115 ,  C12N15/45 ,  C12N15/86 ,  A61K35/76 ,  A61K45/00

CPC分类号： C07K14/005 ,  A61K38/00 ,  A61K48/00 ,  C07K2319/50 ,  C12N15/86 ,  C12N2760/18022 ,  C12N2760/18822 ,  C12N2760/18843 ,  C12N2800/30

摘要： It is intended to provide a cell fusion type vector having replicability with modified protease-dependent tropism. An M gene knockout virus vector encoding a modified F protein, in which the cleavage site of a paramyxovirus F protein has been modified into a sequence cleaved by another protease, is produced. When transferred into cells, this vector replicates genomic RNA carried thereby and thus extends the cell fusion type infection toward adjacent cells depending on the latter protease as described above but does not release any viral particles. Such a vector encoding an F protein cleaved by a protease the activity of which is enhanced due to cancer shows an effect of inhibiting caner growth in vivo.

公开(公告)号：EP1447450A4

公开(公告)日：2005-02-16

申请号：EP02768117

申请日：2002-09-27

申请人： DNAVEC RESEARCH INC

发明人： INOUE MAKOTO ,  IIDA AKIHIRO ,  HASEGAWA MAMORU

IPC分类号： A61K48/00 ,  C12N15/67 ,  C12N15/86 ,  A61K31/60 ,  C12N15/63

CPC分类号： C12N15/86 ,  A61K48/00 ,  C12N15/67 ,  C12N2760/18843 ,  C12N2830/00

摘要： It is found out that a nonsteroidal antiinflammatory agent and a muscle relaxant regulate the expression of a gene carried on a viral vector. These drugs also regulate the cytotoxicity of the viral vector. Thus, a method of regulating the expression of a gene carried on a viral vector with the use of a nonsteroidal antiinflammatory agent and/or a muscle relaxant is provided. The drugs achieve reversible effects. That is, elevated gene expression from the viral vector and an increase in the cytotoxicity of the viral vector are observed after ceasing the administration of the drugs. The above drugs are useful in regulating the expression of a virus gene and a therapeutic gene in gene therapy with the use of a viral vector for gene therapy and regulating the cytotoxicity of the viral vector.

公开(公告)号：EP1715048A4

公开(公告)日：2007-02-07

申请号：EP05703936

申请日：2005-01-20

申请人： DNAVEC RESEARCH INC

发明人： INOUE MAKOTO ,  BAN HIROSHI ,  IIDA AKIHIRO ,  HASEGAWA MAMORU

IPC分类号： C12N15/45 ,  C12N7/00 ,  C12N7/02 ,  C12N15/64

CPC分类号： C12N7/00 ,  C12N2760/18851

摘要： It is intended to provide a process for producing a virus, the proliferation of which depends on the cleavage of a viral protein by a protease, without depending on the protease. This process for producing a virus is characterized by comprising producing the virus in the presence of a viral protein in which the protease cleavage sequence has been modified into the cleavage sequence by another protease. By substituting the inherent protease cleavage sequence by the cleavage sequence by the other protease endogenously expressed by a virus-producing cell, a virus vector can be more efficiently produced. According to this process, a virus with a high titer can be produced by using cells over a wide range.