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    COMPOSITIONS AND METHODS OF USE THEREOF FOR IDENTIFYING ANTI-VIRAL AGENTS
    3.
    发明公开
    COMPOSITIONS AND METHODS OF USE THEREOF FOR IDENTIFYING ANTI-VIRAL AGENTS 有权
    组合物和它们的用途用于鉴定杀病毒

    公开(公告)号：EP2956557A4

    公开(公告)日：2016-09-14

    申请号：EP14751523

    申请日：2014-02-13

    申请人： DAVID GLADSTONE INST

    发明人： WEINBERGER LEOR S

    IPC分类号： C12Q1/70

    CPC分类号： C12N15/86 ,  C12N2710/16122 ,  C12N2710/16143 ,  C12N2740/15043 ,  C12N2830/00 ,  C12N2830/48 ,  C12N2830/60 ,  C12N2840/203 ,  G01N33/5023

    摘要： The present disclosure provides a recombinant expression vector comprising a nucleotide sequence encoding a herpesvirus transactivator, where the nucleotide sequence is operably linked to a herpesvirus control element. The present disclosure provides cell lines genetically modified to express a herpesvirus transactivator under the control of a herpesvirus control element. The present disclosure provides methods of identifying agents that disrupt feedback regulation of a herpesvirus transcriptional control element by a herpesvirus transactivator.

    摘要翻译： 本公开内容提供了包含编码疱疹病毒反式激活，其中所述核苷酸序列可操作地连接到一个控制疱疹病毒元件的核苷酸序列的重组表达载体。 本发明提供了细胞系遗传修饰以表达疱疹病毒控制元件的控制下的反式激活的疱疹病毒。 本发明提供了鉴定剂的方法确实由疱疹病毒反式干扰疱疹病毒的转录控制元件的反馈调节。

    EXPRESSION VECTOR ELEMENT COMBINATIONS, NOVEL PRODUCTION CELL GENERATION METHODS AND THEIR USE FOR THE RECOMBINANT PRODUCTION OF POLYPEPTIDES
    9.
    发明公开
    EXPRESSION VECTOR ELEMENT COMBINATIONS, NOVEL PRODUCTION CELL GENERATION METHODS AND THEIR USE FOR THE RECOMBINANT PRODUCTION OF POLYPEPTIDES 审中-公开
    表达载体的元素组合，生产电池生产及新方法它们在重组多肽生产

    公开(公告)号：EP2794651A2

    公开(公告)日：2014-10-29

    申请号：EP12810245.6

    申请日：2012-12-19

    申请人： F.Hoffmann-La Roche AG

    发明人： HUELSMANN, Peter Michael ,  KNOETGEN, Hendrik

    IPC分类号： C07K16/00 ,  C12N15/67

    CPC分类号： C12N15/85 ,  C07K16/00 ,  C07K16/2854 ,  C07K16/468 ,  C07K2317/14 ,  C12N2830/00 ,  C12N2830/36 ,  C12N2830/42 ,  C12N2840/203

    摘要： Herein is reported that for transient transfections the use of the human elongation factor 1 alpha promoter (with Intron A) provides for an enhanced productivity (in LC-HC-SM organization), the use of the bovine growth hormone polyA signal sequence provides for an enhanced productivity compared to use of the SV40 polyA signal sequence, the addition of the HGT to the bGH PolyA signal sequence results in an increased productivity in vectors containing the hCMV promoter and the vector organization LC(3′-5′)-HC-SM results in improved expression. For stable pools it is reported that pools generated with vectors containing the hEF1α promoter show an enhanced productivity in batch analysis, clones generated with vectors containing the hEF1α promoter show a reduced number of low producing clones, and clones generated with vectors containing the hEF1α promoter show a higher stability of IgG expression. For single clones it is reported that the vector organization with downstream position of selection marker (LC-HC-SM) has a positive effect on productivity of single clones and that clones generated with vectors containing the bGH polyA signal sequence and the hGT have higher productivities.

    摘要翻译： 在报道没有为瞬时转染使用人延伸因子1α启动子（内含子A）的提供用于上提高生产率（在LC-HC-SM组织），使用牛生长激素多聚腺苷酸信号序列提供了用于 相比于使用SV40的增强生产力多聚A信号序列，添加HGT到的bGH polyA信号序列中的生产力提高导致在含有hCMV启动子与矢量组织LC（3'-5“）载体 - HC-SM 导致改进的表达。 对于稳定池有报道并与载体通过含有HEF1α启动子节目以提高生产率在分批分析产生池，用含有HEF1α启动子显示出低生产克隆的数量减少的矢量产生的克隆，并用载体通过含有HEF1α启动子节目产生克隆 IgG表达的更高的稳定性。 对于单克隆有报告做了与选择标记物（LC-HC-SM）的下游位置的矢量组织对单个克隆的生产率具有积极作用，并用含有所述的bGH polyA信号序列和HGT矢量生成并克隆具有更高的生产率 ，