公开(公告)号：EP1499411B1

公开(公告)日：2007-08-08

申请号：EP03727226.7

申请日：2003-05-02

申请人： DSM Biotech GmbH

发明人： RÜFFER, Nicole ,  WANDREY, Christian ,  TAKORS, Ralf

IPC分类号： B01D11/04 ,  C12P13/04

CPC分类号： B01D11/048 ,  B01D11/0488

摘要： The invention relates to a method for the integrated separation of one or more organic substances present in an aqueous bio-process mixture containing at least one positively charged and/or chargeable group containing nitrogen by means of reactive extraction in at least one step. According to the invention, at least one liquid-liquid centrifuge is used for said reactive extraction and the organic substances from the extraction agent are re-extracted into an aqueous phase. In particular, cells and protein are removed from the bio-process mixture that is fed to the liquid-liquid centrifuges before said mixture is fed to the first centrifuge. The invention also relates to a method of this type, whereby an aqueous bio-process mixture is continuously removed from a bio-reactor, fed, together with an extraction agent, to a liquid-liquid centrifuge, extracted by means of the extraction agent, thus obtaining an organic phase, which contains the substance to be extracted. The substance can be re-extracted in a circuit using a second liquid-liquid centrifuge, producing a concentrated aqueous solution from the extracted substance and the organic phase is returned to the first liquid-liquid centrifuge.