摘要:
The present invention relates to a polypeptide having proline-specific endoprotease activity, selected from the group consisting of i. a polypeptide comprising a mature polypeptide sequence of SEQ ID NO: 2; ii. a polypeptide that has least 70%, 75%, 80%, 85%, 86%, 87%, 88%, 89%, 90%, 91 %, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% identity to the mature polypeptide sequence of SEQ ID NO: 2; iii. a polypeptide encoded by a nucleic acid that hybridizes under medium stringency, preferably under high stringency conditions to the complementary strand of the mature polypeptide coding sequence of SEQ ID NO:1; iv. a polypeptide encoded by a nucleic acid that has at least 70%, 75% 80%, 85%, 90%, 91 %, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or 100% identity to the mature polypeptide coding sequence of SEQ ID NO: 1. The invention further relates to a method of producing the polypeptide and a process for the preparation of a food or feed product wherein the polypeptide is used.
摘要翻译:本发明涉及具有脯氨酸特异性内切蛋白酶活性的多肽,其选自i。 包含SEQ ID NO:2的成熟多肽序列的多肽; II。 75%,80%,85%,86%,87%,88%,89%,90%,91%,92%,93%,94%,95%,96% 与SEQ ID NO:2的成熟多肽序列具有97%,98%或99%的同一性; III。 由在中等严格度下优选在高严格条件下与SEQ ID NO:1的成熟多肽编码序列的互补链杂交的核酸编码的多肽; IV。 由核酸编码的多肽,其具有至少70%,75%,80%,85%,90%,91%,92%,93%,94%,95%,96%,97%,98% %或100%的同一性。本发明还涉及生产多肽的方法和制备其中使用多肽的食品或饲料产品的方法。
摘要:
The invention further relates to a polypeptide having proline-specific endoprotease activity comprising an amino acid sequence according to SEQ ID NO: 1, wherein SEQ ID NO: 1 comprises at least one amino acid substitution selected from the group consisting of P469A, P469C, P469D, P469E, P469F, P469G, P469H, P469I, P469K, P469L, P469M, P469N, P469Q, P469R, P469S, P469T, P469V, P469W, P469Y, a nucleic acid encoding a polypeptide having proline-specific endoprotease activity, a method of making a variant polypeptide having proline-specific endoprotease activity, a recombinant host cell and a method of producing the polypeptide and a process for the preparation of a food or feed product wherein the polypeptide is used.
摘要翻译:本发明涉及具有脯氨酸特异性内切蛋白酶活性的多肽,其中当多肽保持在65℃15分钟时,该多肽具有小于70%的残余活性。 本发明还涉及具有脯氨酸特异性内切蛋白酶活性的多肽,其包含根据SEQ ID NO:1的氨基酸序列,其中SEQ ID NO:1包含选自由M144L,S398F,V460A组成的组的至少一个氨基酸取代 ,I204F,Y205N,F279S,F263Y,P377A,L470P和P477A,编码具有脯氨酸特异性内切蛋白酶活性的多肽的核酸,制备具有脯氨酸特异性内切蛋白酶活性的变体多肽的方法,重组宿主细胞和方法 产生多肽的方法和制备其中使用多肽的食物或饲料产品的方法。
摘要:
The invention further relates to a polypeptide having proline-specific endoprotease activity comprising an amino acid sequence according to SEQ ID NO: 1, wherein SEQ ID NO: 1 comprises at least one combination of amino acid substitutions selected from the group consisting of a combination selected from the group consisting of (K238E, I204V, V460A), (F279S, A242V, N507I), (T145A, K424M), (T359A, F379S), (M170I, A421T), (N441S, G484S), (L470H, Q288R), (L470H, E387G), (T281S, L373I), (P304A, P469A), and (P466T, P469Q), a nucleic acid encoding a polypeptide having proline-specific endoprotease activity, a method of making a variant polypeptide having proline-specific endoprotease activity, a recombinant host cell and a method of producing the polypeptide and a process for the preparation of a food or feed product wherein the polypeptide is used.
摘要翻译:本发明涉及具有脯氨酸特异性内切蛋白酶活性的多肽,其中当多肽已经在65℃的温度下保持15分钟时,多肽具有小于70%的残留活性。 本发明进一步涉及具有脯氨酸特异性内切蛋白酶活性的多肽,其包含根据SEQ ID NO:1的氨基酸序列,其中SEQ ID NO:1包含选自以下组合的氨基酸取代的至少一种组合:选自 (T239A,F379S),(M170I,A421T),(N441S,G484S),(L470H, Q288R),(L470H,E387G),(T281S,L373I),(P304A,P469A)和(P466T,P469Q),编码具有脯氨酸特异性内切蛋白酶活性的多肽的核酸, 脯氨酸特异性内切蛋白酶活性,重组宿主细胞和产生多肽的方法以及制备其中使用多肽的食品或饲料产品的方法。