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    PROLINE-SPECIFIC ENDOPROTEASE
    8.
    发明公开
    PROLINE-SPECIFIC ENDOPROTEASE 有权
    PROLINSPEZIFISCHE ENDOPROTEASE

    公开(公告)号：EP3146043A1

    公开(公告)日：2017-03-29

    申请号：EP15724252.0

    申请日：2015-05-19

    申请人： DSM IP Assets B.V.

    发明人： LAAN, VAN DER, Jan Metske ,  VONDERVOORT, VAN DE, Peter Jozef Ida ,  CHRISTIS, Chantal ,  SPAANS, Martine ,  BRUINE-PAULUS, DE, Angela ,  MUTSAERS, Johanna Henrica Gerdina Maria

    IPC分类号： C12N9/50 ,  A23L2/84 ,  A23J3/34 ,  C12N9/62

    CPC分类号： C12N9/62 ,  A23J3/347 ,  A23L2/66 ,  A23L2/84 ,  A23L7/101 ,  A23L33/18 ,  A23L33/195 ,  C12N9/50 ,  C12Y304/21026

    摘要： The present invention relates to a polypeptide having proline-specific endoprotease activity, selected from the group consisting of i. a polypeptide comprising a mature polypeptide sequence of SEQ ID NO: 2; ii. a polypeptide that has least 70%, 75%, 80%, 85%, 86%, 87%, 88%, 89%, 90%, 91 %, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% identity to the mature polypeptide sequence of SEQ ID NO: 2; iii. a polypeptide encoded by a nucleic acid that hybridizes under medium stringency, preferably under high stringency conditions to the complementary strand of the mature polypeptide coding sequence of SEQ ID NO:1; iv. a polypeptide encoded by a nucleic acid that has at least 70%, 75% 80%, 85%, 90%, 91 %, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or 100% identity to the mature polypeptide coding sequence of SEQ ID NO: 1. The invention further relates to a method of producing the polypeptide and a process for the preparation of a food or feed product wherein the polypeptide is used.

    摘要翻译： 本发明涉及具有脯氨酸特异性内切蛋白酶活性的多肽，其选自i。 包含SEQ ID NO：2的成熟多肽序列的多肽; II。 75％，80％，85％，86％，87％，88％，89％，90％，91％，92％，93％，94％，95％，96％ 与SEQ ID NO：2的成熟多肽序列具有97％，98％或99％的同一性; III。 由在中等严格度下优选在高严格条件下与SEQ ID NO：1的成熟多肽编码序列的互补链杂交的核酸编码的多肽; IV。 由核酸编码的多肽，其具有至少70％，75％，80％，85％，90％，91％，92％，93％，94％，95％，96％，97％，98％ ％或100％的同一性。本发明还涉及生产多肽的方法和制备其中使用多肽的食品或饲料产品的方法。

    ASPERGILLUS MUTANT STRAIN AND TRANSFORMANT THEREOF
    9.
    发明公开
    ASPERGILLUS MUTANT STRAIN AND TRANSFORMANT THEREOF 有权
    ASPERGILLUS-MUTANTENSTAMM UND变换DAVON

    公开(公告)号：EP3054004A1

    公开(公告)日：2016-08-10

    申请号：EP16153909.3

    申请日：2016-02-02

    申请人： HONDA MOTOR CO., LTD.

    发明人： SHINKAWA, Satoru ,  MITSUZAWA, Shigenobu ,  TANAKA, Maiko ,  IMAI, Tomohiro

    IPC分类号： C12N1/15 ,  C07K14/38 ,  C12N9/24 ,  C12N9/48 ,  C12N9/62

    CPC分类号： C12N1/14 ,  C07K14/38 ,  C12N9/24 ,  C12N9/2437 ,  C12N9/485 ,  C12N9/62 ,  C12Y302/01091

    摘要： Provided are an Aspergillus mutant strain which can dramatically enhance production of a heterologous enzyme when a saccharifying enzyme gene is transferred into the strain to perform transformation and a transformant having a saccharifying enzyme gene transferred into the Aspergillus mutant strain. The Aspergillus mutant strain has been completely or partially deficient in three genes of Aspergillus oryzae strain HO2 (accession number: NITE BP-01750): a prtR gene coding for a transcription factor; a pepA gene coding for an extracellular acid protease; and a cpI gene coding for an extracellular acid carboxypeptidase.

    摘要翻译： 提供了当将糖化酶基因转移到菌株进行转化时，可以显着提高异源酶的产生的曲霉菌突变菌株和将糖化酶基因转移到曲霉菌突变株中的转化体。 曲霉菌突变菌株在米曲霉菌株HO2（登录号：NITE BP-01750）的三个基因中完全或部分缺陷：编码转录因子的prtR基因; 编码细胞外酸性蛋白酶的pepA基因; 和编码细胞外酸羧肽酶的cpI基因。