公开(公告)号：EP1543134B1

公开(公告)日：2007-04-18

申请号：EP03757854.9

申请日：2003-09-16

申请人： DSM IP Assets B.V.

发明人： Hoshino, Tatsuo ,  Setoguchi, Yutaka ,  Shimizu, Sakayu ,  Tabata, Kazuyuki

IPC分类号： C12P7/26 ,  C12R1/865 ,  C12R1/74 ,  C12R1/645 ,  C12R1/15

CPC分类号： C12R1/645 ,  C12P7/26 ,  C12R1/15 ,  C12R1/74 ,  C12R1/865

摘要： Disclosed is a process for producing actinol from ketoisophorone which comprises contacting ketoisophorone with a recombinant microorganism or cell-free extract thereof in a reaction mixture, wherein said recombinant microorganism is obtainable by transforming a host microorganism, e.g. selected from the group consisting of microorganisms of the genera Saccharomyces, Zygosaccharomyces, and Candida, such as commercially available baker's yeast, Saccharomyces cerevisiae ATCC7754, Saccharomyces rouxii (Zygosaccharomyces rouxii) HUT7191 (IFO 0494), Saccharomyces delbrueckii HUT7116 (Saccharomyces unisporus IFO 0298), Saccharomyces delbrueckii (Torulaspora delbrueckii) HUT7102, Saccharomyces willianus HUT7106, Zygosaccharomyces bailii ATCC11486, Candida tropicalis IFO 1403, and a mutant thereof, which is capable of reducing ketoisophorone to levodione with a levodione reductase gene, e.g. a levodione reductase gene derived from a microorganism belonging to the genus Corynebacterium, such as C. aquaticum AKU611 (FERM BP-6448) or a mutant thereof, and isolating the produced actinol from the reaction mixture.

公开(公告)号：EP1026235B1

公开(公告)日：2008-04-09

申请号：EP00101742.5

申请日：2000-01-28

申请人： DSM IP Assets B.V.

发明人： Nakamori, Shigeru ,  Shimizu, Sakayu ,  Wada, Masaru

IPC分类号： C12N9/02 ,  C12P7/26 ,  C12P41/00 ,  C12R1/15

CPC分类号： C12N9/0008 ,  C12P7/26 ,  C12P41/002

公开(公告)号：EP1543134A2

公开(公告)日：2005-06-22

申请号：EP03757854.9

申请日：2003-09-16

申请人： DSM IP Assets B.V.

发明人： Hoshino, Tatsuo ,  Setoguchi, Yutaka ,  Shimizu, Sakayu ,  Tabata, Kazuyuki

IPC分类号： C12P7/26

CPC分类号： C12R1/645 ,  C12P7/26 ,  C12R1/15 ,  C12R1/74 ,  C12R1/865

摘要： Disclosed is a process for producing actinol from ketoisophorone which comprises contacting ketoisophorone with a recombinant microorganism or cell-free extract thereof in a reaction mixture, wherein said recombinant microorganism is obtainable by transforming a host microorganism, e.g. selected from the group consisting of microorganisms of the genera Saccharomyces, Zygosaccharomyces, and Candida, such as commercially available baker's yeast, Saccharomyces cerevisiae ATCC7754, Saccharomyces rouxii (Zygosaccharomyces rouxii) HUT7191 (IFO 0494), Saccharomyces delbrueckii HUT7116 (Saccharomyces unisporus IFO 0298), Saccharomyces delbrueckii (Torulaspora delbrueckii) HUT7102, Saccharomyces willianus HUT7106, Zygosaccharomyces bailii ATCC11486, Candida tropicalis IFO 1403, and a mutant thereof, which is capable of reducing ketoisophorone to levodione with a levodione reductase gene, e.g. a levodione reductase gene derived from a microorganism belonging to the genus Corynebacterium, such as C. aquaticum AKU611 (FERM BP-6448) or a mutant thereof, and isolating the produced actinol from the reaction mixture.

摘要翻译： 公开了一种由酮基异佛尔酮制备放线醇的方法，该方法包括将酮基异佛尔酮与重组微生物或其无细胞提取物在反应混合物中接触，其中所述重组微生物可通过转化宿主微生物例如甲醇， （Saccharomyces cerevisiae）ATCC7754，鲁氏酵母（Saccharomyces rouxii）（Zygosaccharomyces rouxii），HUT7191（IFO 0494），德氏乳杆菌HUT7116（Saccharomyces unisporus IFO 0298），酵母属假丝酵母属 德氏乳杆菌（Torulaspora delbrueckii）HUT7102，Saccharomyces willianus HUT7106，Zygosaccharomyces bailii ATCC11486，热带假丝酵母IFO 1403及其突变体，其能够将酮异佛尔酮用左旋二酮还原酶基因还原成左旋二酮，例如 来自棒状杆菌属的微生物如水生假丝酵母AKU611（FERM BP-6448）或其突变体的左旋二酮还原酶基因或其突变体，并从反应混合物中分离所产生的放线醇。