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11 条记录 (耗时 0.015 秒)

    Fermentative vitamin C production
    4.
    发明公开
    Fermentative vitamin C production 审中-公开
    发酵维生素C制剂

    公开(公告)号：EP2308965A1

    公开(公告)日：2011-04-13

    申请号：EP10183907.4

    申请日：2006-02-10

    申请人： DSM IP Assets B.V.

    发明人： Beuzelin Ollivier, Marie-Gabrielle ,  Chevreux, Bastien ,  Dalluegge, Manuela ,  Gelder Van, Marina ,  Goese, Markus G. ,  Hauk, Corina ,  Koekman, Bertus Pieter ,  Meury Bechtel, Anja ,  Mouncey, Nigel John ,  Mayer, Anne Françoise ,  Schipper, Dick ,  Toepfer, Christine ,  Vollebregt, Adrianus Wilhelmus Hermanus ,  Lee, Connie ,  Shinjoh, Masako

    IPC分类号： C12N9/04 ,  C12P7/60 ,  C12P17/04

    CPC分类号： C12P17/04 ,  C12N9/0008

    摘要： The present invention relates to newly identified microorganisms capable of direct production of L-ascorbic acid (hereinafter also referred to as Vitamin C). The invention also relates to polynucleotide sequences comprising genes that encode proteins which are involved in the synthesis of Vitamin C. The invention also features polynucleotides comprising the full length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. The present invention also relates to the use of said polynucleotides and polypeptides as biotechnological tools in the production of Vitamin C from microorganisms, whereby a modification of said polynucleotides and/or encoded polypeptides has a direct or indirect impact on yield, production, and/or efficiency of production of the fermentation product in said microorganism. Also included are methods/processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms. The invention also relates to genetically engineered microorganisms and their use for the direct production of Vitamin C.

    摘要翻译： 本发明涉及能够直接生产L-抗坏血酸的新鉴定的微生物（以下也称为维生素C）。 本发明还涉及包含编码参与维生素C合成的蛋白质的基因的多核苷酸序列。本发明还涉及包含新基因及其片段的全长多核苷酸序列的多核苷酸，由多核苷酸和片段编码的新多肽 以及它们的功能等同物。 本发明还涉及所述多核苷酸和多肽在从微生物生产维生素C中作为生物技术工具的用途，其中所述多核苷酸和/或编码多肽的修饰对产量，产量和/或 所述微生物中发酵产物的生产效率。 还包括使用多核苷酸和修饰的多核苷酸序列转化宿主微生物的方法/方法。 本发明还涉及基因工程微生物及其直接生产维生素C的用途。

    Alcohol-aldehyd-dehydrogenases
    6.
    发明公开
    Alcohol-aldehyd-dehydrogenases 失效
    酒精的醛 - 脱氢酶

    公开(公告)号：EP1688499A1

    公开(公告)日：2006-08-09

    申请号：EP06003507.8

    申请日：1997-09-11

    申请人： DSM IP Assets B.V.

    发明人： Asakura, Akira ,  Hoshino, Tatsuo ,  Ojima, Setsuko ,  Shinjoh, Masako ,  Tomiyama, Noribumi

    IPC分类号： C12N15/53 ,  C12N9/04 ,  C12N1/21 ,  C12N5/10 ,  C12P7/24 ,  C12P7/26 ,  C12P7/40 ,  C12P17/00

    CPC分类号： C12P7/24 ,  C12N9/0006 ,  C12P7/26 ,  C12P7/40 ,  C12P7/60 ,  C12P17/00

    摘要： The present invention is directed to a recombinant enzyme preparation having an alcohol and/or aldehyde dehydrogenase activity which comprises one or more enzymatic polypeptide(s) selected from the group consisting of polypeptides which are identified by SEQ ID NO 5, SEQ ID NO 6, SEQ ID NO 7 and SEQ ID NO 8 and chimeric recombinant enzymes between the polypeptides identified by SEQ ID NO 5, SEQ ID NO 6, SEQ ID NO 7 and SEQ ID NO 8 and functional derivatives of the polypeptides identified above which contain addition, insertion, deletion and/or substitution of one or more amino acid residue(s), wherein said enzymatic polypeptides have said alcohol and/or aldehyde dehydrogenase activity, DNA molecules encoding such polypeptides, vectors comprising such DNA molecules, host cells transformed by such vectors and processes for the production of such recombinant enzyme preparations, aldehyds, ketones or carboxylic acids by using such enzyme preparations and specifically 2-keto-L-gulonic acid and more specifically L-ascorbic acid (vitamin C).

    摘要翻译： 本发明涉及具有醇和/或醛脱氢酶活性的重组酶制剂，其包含一种或多种选自下组的酶多肽：由SEQ ID NO 5，SEQ ID NO 6， SEQ ID NO 7和SEQ ID NO 8所示的多肽和在由SEQ ID NO 5，SEQ ID NO 6，SEQ ID NO 7和SEQ ID NO 8所鉴定的多肽之间的嵌合重组酶，以及上述鉴定的多肽的功能性衍生物， ，其中所述酶多肽具有所述醇和/或醛脱氢酶活性的一个或多个氨基酸残基的缺失和/或取代，编码所述多肽的DNA分子，包含此类DNA分子的载体，由所述载体转化的宿主细胞和 通过使用这样的酶制剂并特别是2-酮基-L-古洛糖酸来制备这样的重组酶制剂，醛，酮或羧酸的方法 更具体地说是L-抗坏血酸（维生素C）。

    Microbial production of L-ascorbic acid
    7.
    发明公开
    Microbial production of L-ascorbic acid 有权
    Mikrobielle Herstellung von L-Ascorbinsäure

    公开(公告)号：EP2348113A2

    公开(公告)日：2011-07-27

    申请号：EP10184019.7

    申请日：2004-08-16

    申请人： DSM IP Assets B.V.

    发明人： Berry, Alan ,  Lee, Connie ,  Mayer, Anne Françoise ,  Shinjoh, Masako

    IPC分类号： C12N15/53 ,  C12N15/11 ,  C12N9/02 ,  C12N9/04 ,  C12N15/63 ,  C12N1/21 ,  C12P17/04 ,  C12P7/60

    CPC分类号： C12P17/04 ,  C12N9/0006 ,  C12N9/0008 ,  C12P7/60

    摘要： The present invention discloses an isolated polynucleotide molecule derived from a polynucleotide encoding a polypeptide having L-sorbosone dehydrogenase activity comprising a partial nucleotide sequence of at least 20 consecutive nucleotides of SEQ ID NO:1. The present invention further relates to a process for the production of L-ascorbic acid in high yield, in particular a process using resting cells of a microorganism able to convert given carbon sources into vitamin C. The thus obtained vitamin C may be further processed by purification and/or separation steps.

    摘要翻译： 本发明公开了衍生自编码具有L-山梨糖酮脱氢酶活性的多肽的多核苷酸的分离的多核苷酸分子，其包含SEQ ID NO：1的至少20个连续核苷酸的部分核苷酸序列。 本发明还涉及以高产率生产L-抗坏血酸的方法，特别是使用能够将给定碳源转化为维生素C的微生物的静息细胞的方法。由此获得的维生素C可以进一步由 纯化和/或分离步骤。