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公开(公告)号：EP3289069B1
公开(公告)日：2020-12-09
申请号：EP16786751.4
申请日：2016-04-28
申请人： DAEWOONG CO., LTD.
发明人： KIM, Kyoung-Yun , SUL, Hye-Young , MIN, Kyoung-Min
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公开(公告)号：EP3383889A1
公开(公告)日：2018-10-10
申请号：EP16870958.2
申请日：2016-11-23
申请人： DAEWOONG CO., LTD.
CPC分类号： C12N9/52 , B01D15/362 , B01D15/363 , B01D61/145 , B01D61/147 , B01D61/58 , B01D2315/16 , C07K14/33 , C12Y304/24069
摘要： The present invention relates to a method for producing a botulinum toxin comprising:(a) treating a culture of a botulinum toxin-producing strain with acid to form a botulinum toxin-containing precipitate; (b) adding a buffer to the botulinum toxin-containing precipitate of step (a), followed by clarification by at least one method selected from the group consisting of depth filtration (DF), microfiltration (MF), ultrafiltration (UF), sterile filtration, membrane chromatography (MC) and centrifugation; (c) subjecting the botulinum toxin-containing solution of step (b) to UF diafiltration, ammonium sulfate precipitation or hydrochloric acid precipitation, and then diluting a retentate resulting from the UF diafiltration in a buffer or dissolving a precipitate resulting from the ammonium sulfate precipitation or hydrochloric acid precipitate in a buffer; and (d) subjecting the retentate dilution, ammonium sulfate precipitate solution or hydrochloric acid precipitate solution of step (c) to anion-exchange chromatography (AEX) to purify the botulinum toxin.
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公开(公告)号：EP3289071A1
公开(公告)日：2018-03-07
申请号：EP16786753.0
申请日：2016-04-28
申请人： DAEWOONG CO., LTD.
发明人： KIM, Kyoung-Yun , SUL, Hye-Young , MIN, Kyoung-Min
CPC分类号： C12N9/52 , C07K14/33 , C12N1/20 , C12Y304/24069
摘要： The present invention relates to a medium composition for production of botulinum toxin and, more particularly, to a medium composition for culture of Clostridium sp. capable of producing botulinum toxin. The medium composition of the present invention comprises a porcine peptone and at least one plant-derived peptone selected from the group consisting of a garden pea hydrolysate, a cotton seed hydrolysate and a wheat gluten hydrolysate. When the medium according to the present invention, which contains plant-derived peptones, porcine peptones and minerals, is used for culture of Clostridium botulinum, the growth rate of the bacterium in the medium is higher than that in each of the medium that is in current use and the medium comprising plant-derived peptones alone. In addition, when the medium of the present invention is used, a high concentration of botulinum toxin can be produced by culturing the bacterium in a safe manner
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公开(公告)号：EP3289070A1
公开(公告)日：2018-03-07
申请号：EP16786752.2
申请日：2016-04-28
申请人： Daewoong Co., Ltd.
发明人： KIM, Kyoung-Yun , SUL, Hye-Young , MIN, Kyoung-Min
CPC分类号： A61K39/385 , A61K39/39 , C07K14/33 , C12N1/20
摘要： The present invention relates to a medium composition for production of botulinum toxin and, more particularly, to a medium composition for culture of Clostridium sp. capable of producing botulinum toxin. The medium composition of the present invention comprises a casein hydrolysate and at least one plant-derived peptone selected from the group consisting of a garden pea hydrolysate, a cotton seed hydrolysate and a wheat gluten hydrolysate. When the medium according to the present invention, which contains plant-derived peptones, casein hydrolysates and minerals, is used for culture of Clostridium botulinum, the growth rate of the bacterium in the medium is higher than that in each of the medium that is in current use and the medium comprising plant-derived peptones alone. In addition, when the medium of the present invention is used, a high concentration of botulinum toxin can be produced by culturing the bacterium in a safe manner.
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公开(公告)号：EP4289418A1
公开(公告)日：2023-12-13
申请号：EP22750072.5
申请日：2022-02-07
申请人： Daewoong Co., Ltd.
发明人： SEO, Jeongdeok , KIM, Han-Byul , KIM, Kyoung-Yun
摘要： The present invention relates to a botulinum toxin freeze-dried dosage form composition storable for a long time, comprising botulinum toxin, protamine sulfate, an isotonic agent and a buffer agent, and a botulinum toxin freeze-dried dosage form according to the present invention lowers the inactivation rate of botulinum toxin during freeze-drying, can maintain the stability of botulinum toxin for longer than a conventional botulinum toxin freeze-dried dosage form, forms a uniform particle size so that the effect exhibited at the site of administration is the same and can prevent the side effect in which an excessive toxin administration effect is exhibited only at some sites.
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公开(公告)号：EP3289070B1
公开(公告)日：2020-05-06
申请号：EP16786752.2
申请日：2016-04-28
申请人： Daewoong Co., Ltd.
发明人： KIM, Kyoung-Yun , SUL, Hye-Young , MIN, Kyoung-Min
IPC分类号： C12N1/20 , C12N5/00 , C07K14/33 , A61K39/385 , A61K39/39
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公开(公告)号：EP3289069A1
公开(公告)日：2018-03-07
申请号：EP16786751.4
申请日：2016-04-28
申请人： DAEWOONG CO., LTD.
发明人： KIM, Kyoung-Yun , SUL, Hye-Young , MIN, Kyoung-Min
CPC分类号： C12N9/52 , C07K14/33 , C12N1/20 , C12N5/0025 , C12N2500/76 , C12Y304/24069
摘要： The present invention relates to a medium composition for production of botulinum toxin and, more particularly, to a medium composition for culture of Clostridium sp. capable of producing botulinum toxin. The medium composition of the present invention comprises at least one plant-derived peptone selected from the group consisting of a garden pea hydrolysate, a cotton seed hydrolysate and a wheat gluten hydrolysate. When the medium according to the present invention, which contains plant-derived peptones and minerals, is used for culture of Clostridium botulinum, the growth rate of the bacterium in the medium is about 1.5-2 times higher than that in the medium that is in current use. In addition, when botulinum toxin is produced by culturing the bacterium in the medium, infection with transmissible spongiform encephalopathy (TSE) or the like can be prevented by blocking introduction of animal-derived components.
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