公开(公告)号：EP0220063A3

公开(公告)日：1988-10-05

申请号：EP86308027

申请日：1986-10-16

申请人： Dainippon Pharmaceutical Co., Ltd.

发明人： Yamada, Masaaki ,  Kawata, Shigeo ,  Sunahara, Noriyuki ,  Furuta, Ryuji ,  Yamayoshi, Michiko

IPC分类号： C12P21/00 ,  C07K03/20 ,  G01N33/577

CPC分类号： C07K16/245 ,  C07K14/545

摘要： An antibody, especially a monoclonal antibody, against a human interleukin l polypeptide having a particular amino acid sequence may be produced by forming a hybridoma cell between an antibody-­producing cell of an animal immunized with the poly­peptide and a myeloma cell, cloning the hybridoma cell and producing the anti-polypeptide antibody with a selected clone capable of such production. The antibody can be used for the purification of the polypeptide and for the quantitative determination of human interleukin l by assays such as EIA and RIA.

公开(公告)号：EP0043285B2

公开(公告)日：1988-06-22

申请号：EP81302975.8

申请日：1981-06-30

申请人： Dainippon Pharmaceutical Co., Ltd.

发明人： Sunahara, Noriyuki ,  Naruto Shunsuke ,  Kagemoto, Akira ,  Kurooka, Shigeru ,  Yokogawa, Kanae

IPC分类号： G01N33/531 ,  G01N33/535 ,  G01N33/94

CPC分类号： C07D209/48 ,  C07C69/533 ,  C07C323/00 ,  C07C327/00 ,  G01N33/9473 ,  Y10S435/961 ,  Y10S435/975 ,  Y10S530/806

公开(公告)号：EP0220063A2

公开(公告)日：1987-04-29

申请号：EP86308027.1

申请日：1986-10-16

申请人： Dainippon Pharmaceutical Co., Ltd.

发明人： Yamada, Masaaki ,  Kawata, Shigeo ,  Sunahara, Noriyuki ,  Furuta, Ryuji ,  Yamayoshi, Michiko

IPC分类号： C12P21/00 ,  C07K3/20 ,  G01N33/577

CPC分类号： C07K16/245 ,  C07K14/545

摘要： An antibody, especially a monoclonal antibody, against a human interleukin l polypeptide having a particular amino acid sequence may be produced by forming a hybridoma cell between an antibody-­producing cell of an animal immunized with the poly­peptide and a myeloma cell, cloning the hybridoma cell and producing the anti-polypeptide antibody with a selected clone capable of such production.
The antibody can be used for the purification of the polypeptide and for the quantitative determination of human interleukin l by assays such as EIA and RIA.

摘要翻译： 针对具有特定氨基酸序列的人白介素1多肽的抗体，特别是单克隆抗体可以通过在用多肽免疫的动物的抗体产生细胞和骨髓瘤细胞之间形成杂交瘤细胞来产生，克隆杂交瘤细胞 并用能够进行这种生产的选择的克隆产生抗多肽抗体。 该抗体可用于多肽的纯化和用于通过诸如EIA和RIA的测定法定量测定人白介素1。

公开(公告)号：EP0043285B1

公开(公告)日：1984-01-04

申请号：EP81302975.8

申请日：1981-06-30

申请人： Dainippon Pharmaceutical Co., Ltd.

发明人： Sunahara, Noriyuki ,  Naruto Shunsuke ,  Kagemoto, Akira ,  Kurooka, Shigeru ,  Yokogawa, Kanae

IPC分类号： G01N33/531 ,  G01N33/535 ,  G01N33/94

CPC分类号： C07D209/48 ,  C07C69/533 ,  C07C323/00 ,  C07C327/00 ,  G01N33/9473 ,  Y10S435/961 ,  Y10S435/975 ,  Y10S530/806

公开(公告)号：EP0043285A1

公开(公告)日：1982-01-06

申请号：EP81302975.8

申请日：1981-06-30

申请人： Dainippon Pharmaceutical Co., Ltd.

发明人： Sunahara, Noriyuki ,  Naruto Shunsuke ,  Kagemoto, Akira ,  Kurooka, Shigeru ,  Yokogawa, Kanae

IPC分类号： G01N33/531 ,  G01N33/535 ,  G01N33/94

CPC分类号： C07D209/48 ,  C07C69/533 ,  C07C323/00 ,  C07C327/00 ,  G01N33/9473 ,  Y10S435/961 ,  Y10S435/975 ,  Y10S530/806

摘要： δ-substituted derivatives of valproic acid are provided for conjugation to antigenic compositions, particularly proteins and enzymes.
The antigenic conjugates are employed for the production of antibodies, which find particular use in immunoassays for the determination of valproate.
Also are disclosed a kit containing at least the conjugate and the antibody and determination method of valproate using the kit.

摘要翻译： 提供丙戊酸的δ-取代衍生物用于与抗原组合物，特别是蛋白质和酶的缀合。 抗原共轭物用于生产抗体，其在免疫测定中特别用于测定丙戊酸盐。 还公开了一种试剂盒中至少含有缀合物和抗体的试剂盒和丙戊酸酯的测定方法。