公开(公告)号：EP0209786A3

公开(公告)日：1988-09-28

申请号：EP86109259

申请日：1986-07-07

申请人： BASF Aktiengesellschaft

发明人： Hillen, Heinz, Dr. ,  Moesta, Peter, Dr. ,  Marcinowski, Stefan, Dr.

IPC分类号： C07K03/20

CPC分类号： C07K14/525 ,  Y10S435/803

公开(公告)号：EP0237966A3

公开(公告)日：1988-03-16

申请号：EP87103601

申请日：1987-03-12

申请人： Takeda Chemical Industries, Ltd.

发明人： Kurokawa, Tsumotu ,  Sasada, Reiko ,  Iwane, Makoto ,  Igarashi, Koichi

IPC分类号： C12N15/00 ,  C12P21/02 ,  C07K13/00 ,  C07K03/20

CPC分类号： C07K14/503

摘要： A human basic fibroblast growth factor (hbFGF), which is usable for a cure promoter for burns etc., therapeutic agent for thrombosis etc. or a reagent for promoting cell cultivation, can be produced in a large quantity by cultivating a transformant as transformed with a vector including a DNA coding the hbFGF.

公开(公告)号：EP0184361A3

公开(公告)日：1987-11-04

申请号：EP85308501

申请日：1985-11-22

申请人： BIOPROBE INTERNATIONAL, INC.

发明人： Ngo, That T.

IPC分类号： C08B37/12 ,  C07K17/06 ,  C12N11/10 ,  C07K03/20

CPC分类号： C08F8/30 ,  C12N11/10

摘要： @ Thiol gels are prepared from hydroxyl containing polymers by nucleophilic substitution of a 2-fluoro-1-methylpyridoxy derivative of a hydroxyl containing polymer with a nucleophile which forms with the hydroxyl substituted polymer a sulfhydryl substituted polymer or precursor thereof, which can be readily converted into a thiol gel. The thiol gel can be activated by means of 2,2'-dipyridyl disulfide and reacted with a free sulfhydryl containing biologically active ligand in order to provide an insolubilized ligand which can be used for various purposes including use as an immobilized biologically active material or use as a matrix in covalent chromatography.

公开(公告)号：EP0187386A3

公开(公告)日：1988-01-27

申请号：EP85116637

申请日：1985-12-28

申请人： HOECHST AKTIENGESELLSCHAFT

发明人： Müllner, Hubert, Dr.

IPC分类号： C07K03/02 ,  C07K03/20 ,  A61K37/02

CPC分类号： C07K14/52

公开(公告)号：EP0162462A3

公开(公告)日：1987-05-13

申请号：EP85106307

申请日：1985-05-22

申请人： J.T. Baker Chemical Co.

发明人： Flashner, Michael

IPC分类号： C07K03/20 ,  G01N33/577 ,  A61K39/395 ,  C12P21/00

CPC分类号： C07K16/00 ,  Y10S424/807 ,  Y10S530/864

摘要： A method of chromatographically separating monoclonal antibody type IgM from mouse ascites fluid utilizing a particular chromatographic packing of silica gel bearing bound polyethylenimine functions.

公开(公告)号：EP0184355A3

公开(公告)日：1987-04-08

申请号：EP85308471

申请日：1985-11-21

申请人： ELI LILLY AND COMPANY

发明人： Smith, Michele Ceceil ,  Pidgeon, Charles

IPC分类号： C07K17/02 ,  C07K01/14 ,  C07K03/20

CPC分类号： C12N15/62 ,  B01J20/223 ,  B01J20/286 ,  B01J20/3212 ,  B01J20/3265 ,  B01J2220/49 ,  B01J2220/54 ,  B01J2220/58 ,  C07K1/22 ,  C07K2319/00 ,  C07K2319/20 ,  C07K2319/50

摘要： This invention describes a process for separating a biologically active polypeptide or protein in the form of its precursor from a mixture containing said precursor and impurities, which comprises contacting said precursor with a resin containing immobilized metal ions, said precursor comprising the biologically active polypeptide or protein covalently linked directly or indirectly to an immobilized metal ion chelating peptide, binding said precursor to said resin, and selectively eluting said precursor from said resin. The corresponding precursor compounds also are described.

公开(公告)号：EP0286323A3

公开(公告)日：1988-11-17

申请号：EP88302905

申请日：1988-03-31

申请人： BAXTER TRAVENOL LABORATORIES, INC.

发明人： Griffith Michael J ,  Neslund Gerald ,  Liu Shu-Len

IPC分类号： C07K03/20 ,  A61K35/16

CPC分类号： C07K14/755

摘要： A process for purifying polypeptides comprising contacting an immobilized antibody specifically binding a polypeptide with that polypeptide, desorbing the polypeptide from the antibody which had adsorbed it, eluting the polypeptide from the presence of the antibody, passing the eluted polypeptide through an affinity region capable of binding the polypeptide, binding the polypeptide in the affinity region and passing contaminants through said region, and eluting the purified polypeptide.

公开(公告)号：EP0220063A3

公开(公告)日：1988-10-05

申请号：EP86308027

申请日：1986-10-16

申请人： Dainippon Pharmaceutical Co., Ltd.

发明人： Yamada, Masaaki ,  Kawata, Shigeo ,  Sunahara, Noriyuki ,  Furuta, Ryuji ,  Yamayoshi, Michiko

IPC分类号： C12P21/00 ,  C07K03/20 ,  G01N33/577

CPC分类号： C07K16/245 ,  C07K14/545

摘要： An antibody, especially a monoclonal antibody, against a human interleukin l polypeptide having a particular amino acid sequence may be produced by forming a hybridoma cell between an antibody-­producing cell of an animal immunized with the poly­peptide and a myeloma cell, cloning the hybridoma cell and producing the anti-polypeptide antibody with a selected clone capable of such production. The antibody can be used for the purification of the polypeptide and for the quantitative determination of human interleukin l by assays such as EIA and RIA.

公开(公告)号：EP0206792A3

公开(公告)日：1987-06-10

申请号：EP86304799

申请日：1986-06-23

申请人： MASSEY UNIVERSITY

发明人： Poll, Dick Jacob ,  Harding, David Roger Kay ,  Hancock, William Stephan

IPC分类号： C07K03/20 ,  B01D15/08

CPC分类号： C07K1/16 ,  B01D15/426

摘要： There is described a mobile phase for use in the purification of proteins using reversed phase HPLC on a preparative scale. The mobile phase is an aqueous solution containing .005 to 1 M of a physiologically acceptable acid of the general formula YCOOH where Y is a polar electron withdrawing group capable of hydrogen bonding silanol groups. The solution also contains up to 95% V of a physiologically acceptable organic solvent. A preparative method of purifying proteins such as HSA or BSA using reversed phase HPLC is also described.

公开(公告)号：EP0144028A3

公开(公告)日：1987-05-06

申请号：EP84113841

申请日：1984-11-15

申请人： J.T. Baker Chemical Co.

发明人： Flashner, Michael

IPC分类号： C07K03/20 ,  C12P21/00

CPC分类号： C07K16/065 ,  Y10S530/809

摘要： A method of chromatographically separating monoclonal antibody type IgG from mouse ascites fluid utilizing a particular chromatographic packing of silica gel bearing bound polyethylenimine functions.