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1.发明公开MONITORING PROTEIN TRAFFICKING USING BETA-GALACTOSIDASE REPORTER FRAGMENT COMPLEMENTATION 有权监测β-半乳糖苷记者-FRAGMENTKOMPLEMENTIERUNG的蛋白质运动
公开(公告)号：EP2724158A1
公开(公告)日：2014-04-30
申请号：EP12802981.6
申请日：2012-06-22
CPC分类号： C12Q1/34 , C07K2319/61 , G01N33/5076
摘要： Methods and materials are disclosed for use in an enzyme fragment complementation assay using complementary fragments of β-galactosidase to study the trafficking of proteins in a cell. Compounds that bind to a target peptide have been found to affect protein folding and therefore trafficking. β-Galactosidase fragments, an enzyme donor (ED) and an enzyme acceptor (EA), are fused to a target peptide and to an intracellular compartment protein, wherein the compartment is involved in intracellular trafficking. Contacting the cell with a compound that binds to the target peptide results in enhanced movement of the protein through the cellular trafficking pathway comprised of the endoplasmic reticulum, Golgi apparatus, the plasma membrane, endosomes, etc. Using this approach, compounds that bind to a target peptide and alter its ability to traffic through the normal cellular pathway can be readily detected.
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2.发明授权MONITORING PROTEIN TRAFFICKING USING BETA-GALACTOSIDASE REPORTER FRAGMENT COMPLEMENTATION 有权监测β-半乳糖苷记者-FRAGMENTKOMPLEMENTIERUNG的蛋白质运动
公开(公告)号：EP2724158B1
公开(公告)日：2016-03-23
申请号：EP12802981.6
申请日：2012-06-22
CPC分类号： C12Q1/34 , C07K2319/61 , G01N33/5076
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