公开(公告)号：EP2513318B1

公开(公告)日：2018-01-24

申请号：EP10842620.6

申请日：2010-12-16

申请人： Dow AgroSciences, LLC

发明人： MEADE, Thomas ,  NARVA, Kenneth ,  STORER, Nicholas, P. ,  SHEETS, Joel, J. ,  WOOSLEY, Aaron, T. ,  BURTON, Stephanie, L.

IPC分类号： C12N15/82 ,  A01H5/00 ,  A01H5/10 ,  A01N63/02 ,  A01N65/00

CPC分类号： C12N15/8286 ,  A01N63/02 ,  A01N65/00 ,  Y02A40/162 ,  A01N2300/00

摘要： The subject invention relates in part to stacking Cry1Be toxins along with Cry1Fa toxins to prevent insects from developing resistance towards either toxin by itself. As discussed in more detail herein, the subject pair of proteins is a particularly advantageous combination, as no other pair of proteins is known to provide high levels of control and non-cross-resistant activity against both Spodoptera frugiperda (FAW) and Ostrinia nubilalis (ECB) insects. This dual, non-cross-resistant activity is also advantageous because it can reduce the number of proteins/genes needed to target these insects with multiple, non-cross-resistant proteins. This can reduce or eliminate the need for refuge acreage. Accordingly, the subject invention also relates generally to using four genes to provide three proteins for non-cross-resistant control of a first insect, and three proteins for non-cross-resistant control of a second insect. In preferred embodiments, the targeted insects are FAW and ECB.

公开(公告)号：EP2513314B1

公开(公告)日：2016-09-21

申请号：EP10842614.9

申请日：2010-12-16

申请人： Dow AgroSciences LLC

发明人： MEADE, Thomas ,  NARVA, Kenneth ,  STORER, Nicholas, P. ,  SHEETS, Joel, J. ,  WOOSLEY, Aaron, T. ,  BURTON, Stephanie, L.

IPC分类号： C12N15/82

CPC分类号： C12N15/8286 ,  A01N63/02 ,  Y02A40/162 ,  A01N25/00

摘要： The subject invention includes methods and plants for controlling lepidopteran insects, said plants comprising Cry1Ca insecticidal protein and a Cry1Ab insecticidal protein in combination to delay or prevent development of resistance by the insects.

公开(公告)号：EP2560475A1

公开(公告)日：2013-02-27

申请号：EP11772785.9

申请日：2011-04-22

申请人： Dow AgroSciences, LLC

发明人： NARVA, Kenneth, E. ,  MEADE, Thomas ,  FENCIL, Kristin, J. ,  LI, Huarong ,  HEY, Timothy, D. ,  WOOSLEY, Aaron, T. ,  OLSON, Monica, Britt

IPC分类号： A01H5/00 ,  C12N5/04 ,  A01N37/18

CPC分类号： C12N15/8286 ,  A01G22/00 ,  A01H5/10 ,  A01N37/18 ,  A01N43/50 ,  B32B1/02 ,  B65D85/00 ,  C07K14/325 ,  Y02A40/162 ,  Y10T428/13

摘要： The subject invention relates in part to Cry34Ab/35Ab in combination with Cry6Aa. The subject invention relates in part to the surprising discovery that combinations of Cry34Ab/Cry35Ab and Cry6Aa are useful for preventing development of resistance (to either insecticidal protein system alone) by a corn rootworm (Diabrotica spp.) population. Included within the subject invention are plants producing these insecticidal Cry proteins, which are useful to mitigate concern that a corn rootworm population could develop that would be resistant to either of these insecticidal protein systems alone. Plants (and acreage planted with such plants) that produce these two insecticidal protein systems are included within the scope of the subject invention. The subject invention also relates in part to combinations of Cry34Ab/35Ab and Cry3Aa proteins "triple stacked" with a Cry6Aa protein. Transgenic plants, including corn, comprising a cry6Aa gene, cry34Ab/35Ab genes, and a cry3Aa gene are included within the scope of the subject invention. Thus, such embodiments target rootworms with three modes of action.

摘要翻译： 本主题发明涉及在与Cry6Aa组合部分Cry34Ab / 35AB。 本主题发明部分地涉及令人惊讶的发现所做Cry34Ab / Cry35Ab和Cry6Aa的组合是用于由玉米根虫防治耐药性的发展（单独的任一杀昆虫蛋白系统）有用（叶甲属）种群。 包括在本发明中是植物生产合成杀虫Cry蛋白，这些都是减轻的关注做了一个玉米根虫群可以发展做了单独会是要么耐合成杀虫蛋白系统是有用的。 植物（和种植面积与检查植物）确实产生合成2个杀虫蛋白系统都包括在本发明的范围之内。 本主题发明部分因此涉及具有Cry6Aa蛋白Cry34Ab / 35AB和的Cry3Aa蛋白的结合“三层堆叠”。 转基因植物，包括玉米，包括cry6Aa基因，cry34Ab / 35AB基因，以及基因的Cry3Aa被包括在本发明的范围之内。 因此，调查了实施例中的目标用行动的三种模式根虫。

公开(公告)号：EP2512222A1

公开(公告)日：2012-10-24

申请号：EP10838260.7

申请日：2010-12-16

申请人： Dow AgroSciences LLC

发明人： MEADE, Thomas ,  NARVA, Kenneth ,  STORER, Nicholas, P. ,  SHEETS, Joel, J. ,  WOOSLEY, Aaron, T. ,  BURTON, Stephanie, L.

IPC分类号： A01H5/00 ,  A01H1/00 ,  A01P7/00

CPC分类号： C12N15/8286 ,  A01N63/02 ,  Y02A40/162 ,  A01N65/00 ,  A01N2300/00

摘要： The subject invention includes methods and plants for controlling lepidopteran insects. The plants comprise a Cry1Fa insecticidal protein and a Cry1Ca insecticidal protein, in combination, to delay or prevent development of resistance by the insect(s).

摘要翻译： 本发明包括控制鳞翅目昆虫的方法和植物。 这些植物组合包含Cry1Fa杀虫蛋白和Cry1Ca杀虫蛋白，以延迟或预防昆虫的抗性发展。

公开(公告)号：EP2512221A1

公开(公告)日：2012-10-24

申请号：EP10838259.9

申请日：2010-12-16

申请人： Dow AgroSciences, LLC

发明人： MEADE, Thomas ,  NARVA, Kenneth ,  STORER, Nicholas, P. ,  SHEETS, Joel, J. ,  WOOSLEY, Aaron, T. ,  BURTON, Stephanie, L.

IPC分类号： A01H5/00 ,  A01H1/00 ,  A01P7/00

CPC分类号： C12N15/8286 ,  A01N63/02 ,  C07K14/325 ,  Y02A40/162 ,  A01N65/00 ,  A01N2300/00

摘要： The subject invention includes methods and plants for controlling lepidopteran insects, said plants comprising Cry1Fa and Cry1Da core toxin containing proteins in combination to delay or prevent development of resistance by the insect(s).

公开(公告)号：EP2513316B1

公开(公告)日：2018-11-28

申请号：EP10842618.0

申请日：2010-12-16

申请人： Dow AgroSciences, LLC

发明人： MEADE, Thomas ,  NARVA, Kenneth ,  STORER, Nicholas, P. ,  SHEETS, Joel, J. ,  WOOSLEY, Aaron, T. ,  BURTON, Stephanie, L.

IPC分类号： A01H5/00 ,  C07K14/325 ,  C12N15/82

CPC分类号： C12N15/8286 ,  A01N63/02 ,  Y02A40/162 ,  A01N65/00

摘要： The subject invention includes methods and plants for controlling fall armyworm lepidopteran insects, said plants comprising a Cry1Da insecticidal protein and a Cry1Ca insecticidal protein, and various combinations of other proteins comprising this pair of proteins, to delay or prevent development of resistance by the insects.

公开(公告)号：EP2512219B1

公开(公告)日：2018-10-03

申请号：EP10838257.3

申请日：2010-12-16

申请人： Dow AgroSciences, LLC

发明人： MEADE, Thomas ,  NARVA, Kenneth ,  STORER, Nicholas, P. ,  SHEETS, Joel, J. ,  WOOSLEY, Aaron, T. ,  BURTON, Stephanie, L.

IPC分类号： A01H5/00 ,  A01H1/00 ,  A01P7/00 ,  A01H5/10 ,  C12N15/82 ,  A01N63/02

CPC分类号： C12N15/8286 ,  A01N63/02 ,  Y02A40/162 ,  A01N65/00 ,  A01N2300/00

摘要： The subject invention includes methods and plants for controlling lepidopteran insects, said plants comprising a Vip3Ab insecticidal protein in combination with a Cry 1Fa insecticidal protein to delay or prevent development of resistance by the insect(s).

公开(公告)号：EP2512225B1

公开(公告)日：2017-08-23

申请号：EP10842622.2

申请日：2010-12-16

申请人： Dow AgroSciences, LLC

发明人： MEADE, Thomas ,  NARVA, Kenneth ,  STORER, Nicholas, P. ,  SHEETS, Joel, J. ,  WOOSLEY, Aaron, T. ,  BURTON, Stephanie, L.

IPC分类号： A01H5/00 ,  C07K14/325 ,  C12N15/82

CPC分类号： A01N37/46 ,  A01G22/00 ,  A01N63/02 ,  C12N15/8286 ,  Y02A40/162 ,  A01N65/00

摘要： The subject invention includes methods and plants for controlling fall army worm lepidopteran insects, said plants comprising a V1p3Ab insecticidal protein and a Cry1Ca insecticidal protein, and various combinations of other proteins comprising this pair of proteins, to delay or prevent development of resistance by the insects.

摘要翻译： 本发明包括用于防治秋季军蚁鳞翅目昆虫的方法和植物，所述植物包含V1p3Ab杀虫蛋白和Cry1Ca杀虫蛋白，以及包含该对蛋白的其他蛋白的各种组合以延迟或阻止昆虫产生抗性 。

公开(公告)号：EP2513317A1

公开(公告)日：2012-10-24

申请号：EP10842619.8

申请日：2010-12-16

申请人： Dow AgroSciences, LLC

发明人： MEADE, Thomas ,  NARVA, Kenneth ,  STORER, Nicholas, P. ,  SHEETS, Joel, J. ,  WOOSLEY, Aaron, T. ,  BURTON, Stephanie, L.

IPC分类号： C12N15/82

CPC分类号： C12N15/8286 ,  A01N63/02 ,  C07K14/325 ,  Y02A40/162 ,  A01N65/00

摘要： The subject invention includes methods and plants for controlling fall army worm insects, said plants comprising a Cry1Da insecticidal protein and a Cry1Be insecticidal protein, and various combinations of other proteins comprising this pair of proteins, to delay or prevent development of resistance by the insects.

公开(公告)号：EP2513316A1

公开(公告)日：2012-10-24

申请号：EP10842618.0

申请日：2010-12-16

申请人： Dow AgroSciences, LLC

发明人： MEADE, Thomas ,  NARVA, Kenneth ,  STORER, Nicholas, P. ,  SHEETS, Joel, J. ,  WOOSLEY, Aaron, T. ,  BURTON, Stephanie, L.

IPC分类号： C12N15/82

CPC分类号： C12N15/8286 ,  A01N63/02 ,  Y02A40/162 ,  A01N65/00

摘要： The subject invention includes methods and plants for controlling fall armyworm lepidopteran insects, said plants comprising a Cry1Da insecticidal protein and a Cry1Ca insecticidal protein, and various combinations of other proteins comprising this pair of proteins, to delay or prevent development of resistance by the insects.

摘要翻译： 本发明包括用于控制的情况下粘虫鳞翅目昆虫的方法和植物，所述植物包含Cry1Da杀虫蛋白和Cry1Ca杀虫蛋白，以及其它蛋白，其包含该对蛋白质的各种组合，以延迟或防止抗性的发展由昆虫。