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公开(公告)号:EP2610334A1
公开(公告)日:2013-07-03
申请号:EP13160763.2
申请日:2007-12-07
申请人: Dow AgroSciences LLC
发明人: Lira, Justin M. , Wright, Terry R. , Russell, Sean M. , Merlo, Donald J. , Webb, Steven Robert , Arnold, Nicole L. , Robinson, Andrew E. , Smith, Kelley A.
CPC分类号: C12N15/821 , C12N15/8275
摘要: The subject invention relates to a novel gene referred to herein as DSM-2. This gene was identified in Sterptomyces coelicolor A3. The DSM-2 protein is distantly related to PAT and BAR. The subject invention also provides plant-optimized genes encoding DSM-2 proteins, DSM-2 can be used as a transgenic trait to impart tolerance in plants and plant cells to the herbicides glufosinate and bialaphos. One preferred use of the subject genes are as selectable markers. The use of this gene as a selectable marker in a bacterial system can increase efficiency for plant transformations. Use of DSM-2 as the sole selection marker eliminates the need for an additional medicinal antibiotic marker (such as ampicillin resistance) during cloning. Various other uses are also possible according to the subject invention.
摘要翻译: 本发明涉及本文称为DSM-2的新基因。 该基因在天竺葵A3中鉴定。 DSM-2蛋白与PAT和BAR密切相关。 本发明还提供编码DSM-2蛋白质的植物优化基因,DSM-2可用作转基因性状,以将植物和植物细胞中的耐受性赋予除草剂草铵膦和双丙二酸。 主题基因的一个优选用途是作为选择标记。 在细菌系统中使用该基因作为选择标记可以提高植物转化的效率。 使用DSM-2作为唯一的选择标记,在克隆过程中不需要额外的药用抗生素标记(如氨苄青霉素抗性)。 根据本发明,各种其他用途也是可能的。
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公开(公告)号:EP2610334B1
公开(公告)日:2016-10-26
申请号:EP13160763.2
申请日:2007-12-07
申请人: Dow AgroSciences LLC
发明人: Lira, Justin M. , Wright, Terry R. , Russell, Sean M. , Merlo, Donald J. , Webb, Steven Robert , Arnold, Nicole L. , Robinson, Andrew E. , Smith, Kelley A.
CPC分类号: C12N15/821 , C12N15/8275
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