公开(公告)号：EP3066915A1

公开(公告)日：2016-09-14

申请号：EP16166288.7

申请日：2011-07-29

申请人： Dow AgroSciences LLC

发明人： MERLO, Donald J. ,  RETALLACK, Diane ,  RUSSELL, Sean ,  WOOSLEY, Aaaron ,  MEADE, Tom ,  NARVA, Kenneth E.

IPC分类号： A01H5/00 ,  C12N15/82 ,  C12N15/84 ,  C12N1/21 ,  C12N15/74

CPC分类号： C12N15/8205 ,  C07K14/195 ,  C07K14/325 ,  C12N15/74 ,  C12N15/743 ,  C12N15/8274 ,  C12N15/8286 ,  Y02A40/162

摘要： Agrobacterium strains that harbor transformation-enhancing genes on a plasmid capable of replication independently of the Agrobacterium chromosome, the Ti plasmid, and plant transformation binary vectors, and uses for these Agrobacterium strains are provided. Additionally, Agrobacterium strains that are deficient in DNA recombination functions that result in instability or rearrangement of plant transformation binary vectors, and that harbor transformation-enhancing genes on a plasmid capable of replication independently of the Agrobacterium chromosome, the Ti plasmid, and plant transformation binary vectors, and uses for these strains, are also provided. Further included are Agrobacterium strains that harbor transformation-enhancing genes integrated into the Agrobacterium chromosome at a locus that does not interfere with or otherwise compromise the normal growth and plant transformation ability of the Agrobacterium cells, and uses for these Agrobacterium strains. Plants made using these Agrobacterium strains are also described.

摘要翻译： 提供了在能够独立于农杆菌染色体，Ti质粒和植物转化双向载体复制的质粒上携带转化增强基因的土壤杆菌菌株，以及这些农杆菌菌株的用途。 另外，导致DNA重组功能缺陷的农杆菌菌株导致植物转化双向载体的不稳定或重排，并且能够独立于农杆菌染色体，Ti质粒和植物转化二元体的能够复制的质粒上转录增强基因 还提供了这些菌株的载体和用途。 还包括农杆菌菌株，其在不干扰或以其他方式损害农杆菌细胞的正常生长和植物转化能力的位点处将整合到农杆菌染色体中的转化增强基因并用于这些农杆菌菌株。 还描述了使用这些农杆菌菌株制备的植物。