公开(公告)号：EP1991047A2

公开(公告)日：2008-11-19

申请号：EP07757734.4

申请日：2007-03-01

申请人： Pioneer Hi-Bred International Inc. ,  E.I. DU PONT DE NEMOURS AND COMPANY

发明人： ALLEN, William, B. ,  SHEN, Bo ,  TARCZYNSKI, Mitchell, C. ,  WILLIAMS, Mark, E. ,  ZHENG, Peizhong ,  ZHONG, Gan-yuan

IPC分类号： A01H1/04 ,  C12N15/82

CPC分类号： A01H1/04 ,  C12N9/1029 ,  C12N15/8247 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/156 ,  C12Q2600/172 ,  Y10T436/104165

摘要： Compositions related to the quantitative trait locus 6 (QTL6) in maize and methods for their use are provided. The compositions are novel molecular marker loci that are genetically linked with QTL6 and which are associated with increased oil content and/or increased oleic acid content and/or an increased oleic acid/linoleic acid ratio of a plant or plant part thereof. These novel markers are characterized by the presence of at least one polymorphism relative to the corresponding marker locus from the QTL6 region of non-high-oil, non-high-oleic acid maize plants. In some embodiments, the novel marker loci comprise coding sequence for a maize DGAT 1-2 polypeptide or biologically active variant thereof. The marker loci of the invention, and suitable fragments thereof, are useful in methods of the invention for manipulating oil and/or oleic acid content and/or oleic acid/lineolic acid ratio of a plant or plant part thereof, for marker-assisted selection of a plant, for example, a maize plant, or plant part thereof, having an increased oil content and/or increased oleic acid content and/or an increased oleic acid/linoleic acid ratio, and for marker- assisted breeding of the high oil and/or high oleic acid trait.

公开(公告)号：EP1412375B1

公开(公告)日：2011-01-12

申请号：EP02752280.4

申请日：2002-06-27

申请人： E.I. DU PONT DE NEMOURS AND COMPANY ,  PIONEER HI-BRED INTERNATIONAL, INC.

发明人： ALLEN, William, B. ,  CAHOON, Rebecca, E. ,  FAMODU, Omolayo, O. ,  HARVELL, Leslie, T. ,  HELENTJARIS, Timothy, G. ,  LI, Changjiang ,  LOWE, Keith, S. ,  OLIVEIRA, Igor, Cunha ,  SHEN, Bo ,  TARCZYNSKI, Mitchell, C.

IPC分类号： C12N15/82 ,  C07K14/415

CPC分类号： C12N15/8247 ,  C07K14/415

摘要： The preparation and use of nucleic acid fragments useful in altering the oil phenotype in plants are disclosed. Recombinant DNA construct incorporating such nucleic acid fragments and suitable regulatory sequences can be used to create transgenic plants having altered lipid profiles. Methods for altering the oil phenotype in plants using such nucleic acid fragments also are disclosed.

公开(公告)号：EP1991047B1

公开(公告)日：2012-09-26

申请号：EP07757734.4

申请日：2007-03-01

申请人： Pioneer Hi-Bred International Inc. ,  E.I. DU PONT DE NEMOURS AND COMPANY

发明人： ALLEN, William, B. ,  SHEN, Bo ,  TARCZYNSKI, Mitchell, C. ,  WILLIAMS, Mark, E. ,  ZHENG, Peizhong ,  ZHONG, Gan-yuan

IPC分类号： A01H1/04 ,  C12N15/82

CPC分类号： A01H1/04 ,  C12N9/1029 ,  C12N15/8247 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/156 ,  C12Q2600/172 ,  Y10T436/104165

公开(公告)号：EP1989314A2

公开(公告)日：2008-11-12

申请号：EP07757727.8

申请日：2007-03-01

申请人： Pioneer Hi-Bred International Inc. ,  E.I. DU PONT DE NEMOURS AND COMPANY

发明人： ALLEN, William, B. ,  SHEN, Bo ,  TARCZYNSKI, Mitchell, C. ,  WILLIAMS, Mark, E. ,  ZHENG, Peizhong ,  ZHONG, Gan-yuan

IPC分类号： C12N15/82 ,  C12N15/54 ,  C12P7/64 ,  A01H5/00

CPC分类号： C12N15/8247 ,  C12N9/1029 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/156 ,  C12Q2600/172

摘要： Compositions related to the quantitative trait locus 6 (QTL6) in maize and methods for their use are provided. The compositions are novel molecular marker loci that are genetically linked with QTL6 and which are associated with increased oil content and/or increased oleic acid content and/or an increased oleic acid/linoleic acid ratio of a plant or plant part thereof. These novel markers are characterized by the presence of at least one polymorphism relative to the corresponding marker locus from the QTL6 region of non-high-oil, non-high-oleic acid maize plants. In some embodiments, the novel marker loci comprise coding sequence for a maize DGAT 1-2 polypeptide or biologically active variant thereof. The marker loci of the invention, and suitable fragments thereof, are useful in methods of the invention for manipulating oil and/or oleic acid content and/or oleic acid/lineolic acid ratio of a plant or plant part thereof, for marker-assisted selection of a plant, for example, a maize plant, or plant part thereof, having an increased oil content and/or increased oleic acid content and/or an increased oleic acid/linoleic acid ratio, and for marker- assisted breeding of the high oil and/or high oleic acid trait.

摘要翻译： 提供了玉米中与数量性状基因座6（QTL6）相关的组合物及其使用方法。 该组合物是新的分子标记基因座，其与QTL6遗传连锁并且与植物或其植物部分的油含量增加和/或油酸含量增加和/或油酸/亚油酸比例增加有关。 这些新标记的特征在于存在相对于来自非高油非高油酸玉米植物的QTL6区的相应标记基因座的至少一种多态性。 在一些实施方案中，新标记基因座包含玉米DGAT 1-2多肽或其生物活性变体的编码序列。 本发明的标志物基因座及其合适的片段可用于本发明的用于操作植物或其植物部分的油和/或油酸含量和/或油酸/线酸比率的方法，用于标记辅助选择 例如具有增加的油含量和/或增加的油酸含量和/或增加的油酸/亚油酸比率的植物，例如玉米植物或其植物部分，以及用于标记辅助育种高油 和/或高油酸性状。

公开(公告)号：EP1412373A2

公开(公告)日：2004-04-28

申请号：EP02749657.9

申请日：2002-06-27

申请人： E. I. du Pont de Nemours and Company ,  PIONEER HI-BRED INTERNATIONAL, INC.

发明人： ALLEN, Stephen, M. ,  ALLEN, William, B. ,  CAHOON, Rebecca, E. ,  EPELBAUM, Sabine ,  FAMODU, Omolayo, O. ,  HARVELL, Leslie, T. ,  JONES, Todd, J. ,  KINNEY, Anthony, J. ,  KLEIN, Theodore, M. ,  LI, Changjiang ,  OLIVEIRA, Igor, Cunha ,  SAKAI, Hajime ,  SHEN, Bo ,  TARCZYNSKI, Mitchell, C.

IPC分类号： C07H21/04

CPC分类号： C12N15/8247 ,  C07K14/415

摘要： The preparation and use of nucleic acid fragments useful in altering the oil phenotype in plants are disclosed. Chimeric construct incorporating such nucleic acid fragments and suitable regulatory sequences can be used to create transgenic plants having altered lipid profiles. Methods for altering the oil phenotype in plants using such nucleic acid fragments also are disclosed.

公开(公告)号：EP1412375A2

公开(公告)日：2004-04-28

申请号：EP02752280.4

申请日：2002-06-27

申请人： E. I. du Pont de Nemours and Company ,  PIONEER HI-BRED INTERNATIONAL, INC.

发明人： ALLEN, William, B. ,  CAHOON, Rebecca, E. ,  FAMODU, Omolayo, O. ,  HARVELL, Leslie, T. ,  HELENTJARIS, Timothy, G. ,  LI, Changjiang ,  LOWE, Keith, S. ,  OLIVEIRA, Igor, Cunha ,  SHEN, Bo ,  TARCZYNSKI, Mitchell, C.

IPC分类号： C07H21/04

CPC分类号： C12N15/8247 ,  C07K14/415

摘要： The preparation and use of nucleic acid fragments useful in altering the oil phenotype in plants are disclosed. Recombinant DNA construct incorporating such nucleic acid fragments and suitable regulatory sequences can be used to create transgenic plants having altered lipid profiles. Methods for altering the oil phenotype in plants using such nucleic acid fragments also are disclosed.