公开(公告)号：EP1564299A4

公开(公告)日：2006-07-05

申请号：EP03774148

申请日：2003-11-21

申请人： EISAI CO LTD ,  NAT INST OF ADVANCED IND SCIEN

发明人： TSUKAHARA KAPPEI ,  TSUCHIYA MAMIKO ,  JIGAMI YOSHIFUMI ,  NAKAYAMA KENICHI ,  UMEMURA MARIKO ,  OKAMOTO MICHIYO

IPC分类号： C12N15/55 ,  C12Q1/18 ,  C12Q1/48 ,  C12Q1/02 ,  G01N33/15 ,  G01N33/50

CPC分类号： C12Q1/48 ,  C12Q1/18

摘要： It becomes possible to screen a compound inhibiting the transportation of a GPI anchor protein into fungal cell wall by a convenient method of assaying transacylation to GlcN-PI with the use of a membrane fraction expressing a GWT1 protein. By inhibiting the process of transporting the GPI anchor protein into cell wall, a novel antifungal agent, which inhibits the synthesis of fungal cell wall and, at the same time, inhibits adhesion to host cells, can be designed.

公开(公告)号：EP1300464A4

公开(公告)日：2006-02-01

申请号：EP01947898

申请日：2001-07-06

申请人： EISAI CO LTD

发明人： TSUKAHARA KAPPEI ,  HATA KATSURA ,  SAGANE KOJI ,  NAKAMOTO KAZUTAKA ,  TSUCHIYA MAMIKO ,  WATANABE NAOAKI ,  OBA FUMINORI ,  TSUKADA ITARU ,  UEDA NORIHIRO ,  TANAKA KEIGO ,  KAI JUNKO

IPC分类号： A61K31/00 ,  A61K31/4355 ,  A61K31/4365 ,  A61K31/437 ,  A61K31/44 ,  A61K31/472 ,  A61K31/4725 ,  A61K31/5377 ,  A61K38/00 ,  A61P31/10 ,  C07D213/68 ,  C07D217/18 ,  C07D217/20 ,  C07D491/048 ,  C07D491/056 ,  C07K14/37 ,  C07K14/38 ,  C07K14/39 ,  C07K14/395 ,  C07K14/40 ,  C12N15/09 ,  A61K39/395 ,  A61K45/00 ,  C07K16/14 ,  C12N15/31 ,  G01N33/15 ,  G01N33/50

CPC分类号： C07K14/40 ,  A61K31/00 ,  A61K31/4355 ,  A61K31/4365 ,  A61K31/437 ,  A61K31/44 ,  A61K31/472 ,  A61K31/4725 ,  A61K31/5377 ,  A61K38/00 ,  C07D213/68 ,  C07D217/18 ,  C07D217/20 ,  C07K14/37 ,  C07K14/38 ,  C07K14/39 ,  C07K14/395 ,  C07K2319/02 ,  G01N2500/00 ,  Y10S435/911

摘要： A reporter system reflecting the transport process of GPI anchor protein to cell wall is constructed and a compound inhibiting this process is found out. Further, a gene imparting tolerance to the above compound is identified and a method of screening a compound inhibiting the activity of the protein encoded by this gene is developed. Thus, it is clarified by the novel compound that antifungal agents depending on a novel mechanism, wherein the transport process of GPI anchor protein to cell wall is inhibited, are available.