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    All-in-one sample preparation device and method
    2.
    发明公开
    All-in-one sample preparation device and method 审中-公开
    装置和方法的完整样品制备

    公开(公告)号：EP2546346A3

    公开(公告)日：2014-03-12

    申请号：EP12175469.1

    申请日：2012-07-06

    申请人： EMD Millipore Corporation

    发明人： Scott, Christopher A. ,  Greenizen, Kurt ,  Gutierrez, Sara D. ,  Briggs, David ,  Scaduto, Ralph T. ,  Nadler, Timothy K. ,  Bonhomme, Luis ,  Mabuchi, Masaharu ,  Clark, Phillip

    IPC分类号： C12N15/10 ,  B01L3/14

    摘要： Sample preparation device that allows for a complete bind, wash, elute, buffer-exchange and concentration process to be carried out without sample transfer between multiple devices. The device includes a reservoir, a column for holding chromatography media, a holder region for holding a filtration device, and an outlet. The filtration device plugs into the holder region of the centrifugal device, and the assembly can be placed in an optional holder. The assembly, with or without the optional holder, can be placed in a conventional centrifuge tube for centrifugation. The entire bind, wash, elute, buffer exchange and concentration steps can be carried out with the apparatus without any pipette transfers (and the associated sample losses. The sample preparation device also can be used for binding and washing steps, in which case the filtration device is not needed, and for buffer exchange and concentration steps, in which case the media is not needed.

    All-in-one sample preparation device and method
    5.
    发明公开
    All-in-one sample preparation device and method 审中-公开
    Vorrichtung und Verfahren zurvollständigenProbenvorbereitung

    公开(公告)号：EP2546346A2

    公开(公告)日：2013-01-16

    申请号：EP12175469.1

    申请日：2012-07-06

    申请人： EMD Millipore Corporation

    发明人： Scott, Christopher A. ,  Greenizen, Kurt ,  Gutierrez, Sara D. ,  Briggs, David ,  Scaduto, Ralph T. ,  Nadler, Timothy K. ,  Bonhomme, Luis ,  Mabuchi, Masaharu ,  Clark, Philip

    IPC分类号： C12N15/10 ,  B01L3/14

    摘要： Sample preparation device that allows for a complete bind, wash, elute, buffer-exchange and concentration process to be carried out without sample transfer between multiple devices. The device includes a reservoir, a column for holding chromatography media, a holder region for holding a filtration device, and an outlet. The filtration device plugs into the holder region of the centrifugal device, and the assembly can be placed in an optional holder. The assembly, with or without the optional holder, can be placed in a conventional centrifuge tube for centrifugation. The entire bind, wash, elute, buffer exchange and concentration steps can be carried out with the apparatus without any pipette transfers (and the associated sample losses. The sample preparation device also can be used for binding and washing steps, in which case the filtration device is not needed, and for buffer exchange and concentration steps, in which case the media is not needed.

    摘要翻译： 允许完全结合，洗涤，洗脱，缓冲交换和浓缩过程的样品制备装置，在多个装置之间不进行样品转移。 该装置包括储存器，用于保持色谱介质的柱，用于保持过滤装置的保持器区域和出口。 过滤装置插入离心装置的保持器区域，并且组件可以放置在可选的保持器中。 具有或不具有任选的保持器的组件可以放置在用于离心的常规离心管中。 整个结合，洗涤，洗脱，缓冲液交换和浓缩步骤可以在没有任何移液管转移（和相关样品损失）的情况下进行，样品制备装置也可用于结合和洗涤步骤，在这种情况下，过滤 设备不需要，并且用于缓冲区交换和集中步骤，在这种情况下不需要介质。