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1.发明授权Intrabodies ScFv with defined framework that is stable in a reducing environment 有权ScFv的胞内抗体与定义的结构（“框架”），这是在还原性环境中是稳定
公开(公告)号：EP1479694B1
公开(公告)日：2016-10-26
申请号：EP04017280.1
申请日：2000-12-18
IPC分类号： C07K16/00 , C12N15/10 , C12N15/13 , A61K39/395 , C07K19/00 , G01N33/577
CPC分类号： C07K16/005 , A61K2039/505 , C07K16/00 , C07K16/14 , C07K2317/622 , C07K2317/80 , C07K2318/10 , C07K2319/00 , C12N15/1055 , C12N15/1093
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公开(公告)号：EP2947095B1
公开(公告)日：2019-10-02
申请号：EP15160739.7
申请日：2003-05-21
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3.发明授权Immunoglobulin frameworks which demonstrate enhanced stability in the intracellular environment and methods of identifying same 有权在细胞内空间具有改善的稳定性和Immunoglobulinrahmen识别方法
公开(公告)号：EP2332989B1
公开(公告)日：2015-04-01
申请号：EP10009857.3
申请日：2003-05-21
CPC分类号： C07K16/00 , A61K48/00 , C07K2317/21 , C07K2317/55 , C07K2317/567 , C07K2317/622 , C07K2317/80 , C07K2317/82 , C07K2317/94 , G01N33/6857
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4.发明公开Immunoglobulin frameworks which demonstrate enhanced stability in the intracellular environment and methods of identifying same 有权在细胞内空间具有改善的稳定性和Immunoglobulinrahmen识别方法
公开(公告)号：EP2332989A1
公开(公告)日：2011-06-15
申请号：EP10009857.3
申请日：2003-05-21
CPC分类号： C07K16/00 , A61K48/00 , C07K2317/21 , C07K2317/55 , C07K2317/567 , C07K2317/622 , C07K2317/80 , C07K2317/82 , C07K2317/94 , G01N33/6857
摘要： A single chain variable framework has been selected for intracellular performance which may be used in diagnostic and therapeutic applications, target validation and gene therapy.
摘要翻译： 单链可变框架已被选择为可在诊断和治疗应用，靶标确认和基因治疗中使用的细胞内的性能。
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5.发明公开Intrabodies with defined framework that is stable in a reducing environment and applications thereof 审中-公开用限定的结构（“框架”），其是稳定在还原环境中，并且它们的用途胞内抗体
公开(公告)号：EP2392596A3
公开(公告)日：2013-11-27
申请号：EP10010030.4
申请日：2000-12-18
IPC分类号： C07K16/00 , C12N15/10 , C12N15/13 , A61K39/395 , C07K19/00 , G01N33/577
CPC分类号： C07K16/005 , A61K2039/505 , C07K16/00 , C07K16/14 , C07K2317/622 , C07K2317/80 , C07K2318/10 , C07K2319/00 , C12N15/1055 , C12N15/1093
摘要： Methods for the generation of a CDR library with a defined framework that is stable and soluble in a reducing environment are described as well as thus obtained library of intrabodies.
摘要翻译： 一种用于CDR的在限定的框架的隔离方法确实是稳定的和可溶于还原环境被描述，以及由此获得scFv的。 寻求的scFv与定义的框架可以worin框架来生成scFv文库开始是保守的，而至少一个互补决定区（CDR）是随机的。 搜索库，E.G. 在酵母细胞中，适合用于筛选抗体/ CDR-相互作用或用于筛选的抗体。
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6.发明公开
公开(公告)号：EP2314622A1
公开(公告)日：2011-04-27
申请号：EP10010455.3
申请日：2003-05-21
CPC分类号： C07K16/00 , A61K48/00 , C07K2317/21 , C07K2317/55 , C07K2317/567 , C07K2317/622 , C07K2317/80 , C07K2317/82 , C07K2317/94 , G01N33/6857
摘要： Compositions are provided, which can be used as frameworks for the creation of very stable and soluble single-chain Fv antibody fragments. These frameworks have been selected for intracellular performance and are thus ideally suited for the creation of scFv antibody fragments or scFv antibody libraries for applications where stability and solubility are limiting factors for the performance of antibody fragments, such as in the reducing environment of a cell. Such frameworks can also be used to identify highly conserved residues and consensus sequences which demonstrate enhanced solubility and stability.
摘要翻译： 提供了组合物，其可以被用作用于创建非常稳定的和可溶的单链Fv抗体片段的框架。 这些框架已被选定为细胞内的性能，并且因此非常适合用于创建scFv抗体片段或scFv抗体文库的为其中的稳定性和溶解性的限制因素为抗体片段，：如在细胞的还原环境的应用程序的性能。 因此Web框架可以被用于识别高度保守的残基，并且其证明增强的溶解性和稳定性的共有序列。
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7.发明公开Stable and soluble antibodies inhibiting TNF(alpha) 有权Stabile undlöslicheAntikörperzur Hemmung von TNF（alpha）
公开(公告)号：EP2390267A1
公开(公告)日：2011-11-30
申请号：EP11002906.3
申请日：2006-06-06
CPC分类号： C07K16/241 , A61K2039/505 , C07K2317/14 , C07K2317/21 , C07K2317/24 , C07K2317/522 , C07K2317/55 , C07K2317/56 , C07K2317/565 , C07K2317/567 , C07K2317/622 , C07K2317/76 , C07K2317/92 , C07K2317/94
摘要： The present invention relates to particularly stable and soluble scFv antibodies and Fab fragments specific for TNFα, which comprise specific light chain and heavy chain sequences that are optimized for stability, solubility, in vitro and in vivo binding of TNFα, and low immunogenicity. Said antibodies are designed for the diagnosis and/or treatment of TNFα-related disorders. The nucleic acids, vectors and host cells for expression of the recombinant antibodies of the invention, methods for isolating them and the use of said antibodies in medicine are also disclosed.
摘要翻译： 本发明涉及特别稳定和可溶的scFv抗体和针对TNF±特异性的Fab片段，其包含针对TNF±的稳定性，溶解性，体外和体内结合优化的特异性轻链和重链序列，以及低免疫原性。 所述抗体被设计用于诊断和/或治疗TNF-相关病症。 还公开了用于表达本发明的重组抗体的核酸，载体和宿主细胞，用于分离它们的方法和所述抗体在医学中的用途。
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8.发明公开Immunoglobulin frameworks which demonstrate enhanced stability in the intracellular environment and methods of identifying same 审中-公开稳定性改进帧内细胞环境和方法识别IT免疫球蛋白FRAME
公开(公告)号：EP2947095A1
公开(公告)日：2015-11-25
申请号：EP15160739.7
申请日：2003-05-21
摘要： A single chain variable framework has been selected for intracellular performance which may be used in diagnostic and therapeutic applications, target validation and gene therapy.
摘要翻译： 单链可变框架已被选定为可在诊断和治疗应用，靶标确认和基因治疗中使用的细胞内的性能。
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9.发明公开Intrabodies with defined framework that is stable in a reducing environment and applications thereof 审中-公开用限定的结构（“框架”），其是稳定在还原环境中，并且它们的用途胞内抗体
公开(公告)号：EP2392596A2
公开(公告)日：2011-12-07
申请号：EP10010030.4
申请日：2000-12-18
IPC分类号： C07K16/00 , C12N15/10 , C12N15/13 , A61K39/395 , C07K19/00 , G01N33/577
CPC分类号： C07K16/005 , A61K2039/505 , C07K16/00 , C07K16/14 , C07K2317/622 , C07K2317/80 , C07K2318/10 , C07K2319/00 , C12N15/1055 , C12N15/1093
摘要： Methods for the generation of a CDR library with a defined framework that is stable and soluble in a reducing environment are described as well as thus obtained library of intrabodies.
摘要翻译： 对于CDR库定义的框架的生成方法所做的就是稳定，易溶于还原环境中被描述，以及细胞内抗体由此获得库。
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10.发明公开Intrabodies ScFv with defined framework that is stable in a reducing environment 有权ScFvIntrakörpermit definierter Struktur（“Framework”），在einer reduzierenden Umgebung stable ist
公开(公告)号：EP1479694A3
公开(公告)日：2010-05-05
申请号：EP04017280.1
申请日：2000-12-18
IPC分类号： C07K16/00 , C12N15/10 , C12N15/13 , A61K39/395 , C07K19/00 , G01N33/577
CPC分类号： C07K16/005 , A61K2039/505 , C07K16/00 , C07K16/14 , C07K2317/622 , C07K2317/80 , C07K2318/10 , C07K2319/00 , C12N15/1055 , C12N15/1093
摘要： A method for the isolation of CDRs in a defined framework that is stable and soluble in reducing environment is described as well as thus obtainable scFv. Starting from such scFv with defined framework a scFv library can be generated wherein the framework is conserved while at least one complementary determining region (CDR) is randomized. Such library, e.g. in yeast cells, is suitable for screening for antibody/CDR-interactions or for screening for antibodies.
摘要翻译： 描述了在限定的框架中分离CDR的方法，其稳定和可溶于还原环境，以及由此获得的scFv。 从具有定义框架的这种scFv开始，可以生成scFv文库，其中框架是保守的，而至少一个互补决定区（CDR）是随机的。 这样的文库，例如 在酵母细胞中，适用于筛选抗体/ CDR相互作用或筛选抗体。
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