公开(公告)号：EP0815129B1

公开(公告)日：2011-01-05

申请号：EP96905368.5

申请日：1996-02-12

申请人： Eastern Virginia Medical School of the Medical College of Hampton Roads ,  McGill University

发明人： VINIK, Aaron, I. ,  PITTENGER, Gary, L. ,  RAFAELOFF, Ronit ,  ROSENBERG, Lawrence ,  DUGUID, William, P.

IPC分类号： C12N15/12 ,  C07K14/00 ,  C07K16/00 ,  C12N5/00 ,  C12N15/00 ,  C07H21/00 ,  A61K31/00 ,  A61K38/00 ,  A61K48/00 ,  C12Q1/68 ,  C12P19/34

CPC分类号： C07K14/474 ,  A01K2217/05 ,  A61K38/00 ,  C07K14/4733 ,  C07K2319/00

摘要： Cellophane wrapping (CW) of hamster pancreas induces proliferation of duct epithelial cells followed by endocrine cell differentiation and islet neogenesis. Using the mRNA differential display technique a cDNA clone expressed in cellophane wrap but not in control pancreata was identified. Using this cDNA as a probe, a cDNA library was screened and a gene not previously described was identified and named INGAP.

公开(公告)号：EP0815129A1

公开(公告)日：1998-01-07

申请号：EP96905368.0

申请日：1996-02-12

申请人： Eastern Virginia Medical School of the Medical College of Hampton Roads ,  McGILL UNIVERSITY

发明人： VINIK, Aaron, I. ,  PITTENGER, Gary, L. ,  RAFAELOFF, Ronit ,  ROSENBERG, Lawrence ,  DUGUID, William, P.

IPC分类号： C07K14 ,  A61K38

CPC分类号： C07K14/474 ,  A01K2217/05 ,  A61K38/00 ,  C07K14/4733 ,  C07K2319/00

摘要： Cellophane wrapping (CW) of hamster pancreas induces proliferation of duct epithelial cells followed by endocrine cell differentiation and islet neogenesis. Using the mRNA differential display technique a cDNA clone expressed in cellophane wrap but not in control pancreata was identified. Using this cDNA as a probe, a cDNA library was screened and a gene not previously described was identified and named INGAP.

公开(公告)号：EP1007647B1

公开(公告)日：2004-05-06

申请号：EP97912942.6

申请日：1997-10-30

申请人： Eastern Virginia Medical School of the Medical College of Hampton Roads

发明人： VINIK, Aaron, I. ,  PITTENGER, Gary, I. ,  RAFAELOFF, Ronit ,  BARLOW, Scott, W.

IPC分类号： C12N15/00 ,  C07H21/04 ,  C07K1/22 ,  C07K14/47

CPC分类号： C07K14/4733 ,  C07K2319/00

摘要： Removal of the nucleotide sequence encoding the signal peptide from the INGAP coding sequence allows bacteria to express substantial amounts of INGAP activity. Previous attempts have provided only low yields of INGAP, possibly because the signal sequence of INGAP is toxic to bacteria.

公开(公告)号：EP1007647A1

公开(公告)日：2000-06-14

申请号：EP97912942.6

申请日：1997-10-30

申请人： Eastern Virginia Medical School of the Medical College of Hampton Roads

发明人： VINIK, Aaron, I. ,  PITTENGER, Gary, I. ,  RAFAELOFF, Ronit ,  BARLOW, Scott, W.

IPC分类号： C12N15/00 ,  C07H21/04 ,  C07K1/22

CPC分类号： C07K14/4733 ,  C07K2319/00

摘要： Removal of the nucleotide sequence encoding the signal peptide from the INGAP coding sequence allows bacteria to express substantial amounts of INGAP activity. Previous attempts have provided only low yields of INGAP, possibly because the signal sequence of INGAP is toxic to bacteria.