公开(公告)号：EP4086341A1

公开(公告)日：2022-11-09

申请号：EP20911101.2

申请日：2020-12-30

申请人： Edigene Biotechnology Inc.

发明人： YUAN, Pengfei ,  WANG, Fei ,  NIU, Lichao ,  FU, Jianqiang ,  GU, Feng

IPC分类号： C12N5/10 ,  C12N15/113 ,  C07K16/28 ,  A61K35/17

摘要： A method for purifying a universal human CAR-T cell, comprising: (i) destroying, by using gene editing technology, a TRAC gene region from position 23016448 to position 23016490 of chromosome 14 and a B2M gene region from position 45003745 to position 4500378 of chromosome 15 in the human CAR-T cell; (ii) then introducing a mRNA targeting TCRα/β into the gene-edited CAR-T cell; and (iii) in vitro culturing the CAR-T cell population after the described treatment.

公开(公告)号：EP4086340A1

公开(公告)日：2022-11-09

申请号：EP20910719.2

申请日：2020-12-28

申请人： Edigene Biotechnology Inc.

发明人： WANG, Fei ,  YUAN, Pengfei ,  NIU, Lichao ,  FU, Jianqiang ,  WU, Lanlan

IPC分类号： C12N5/10 ,  C12N15/113

摘要： A novel preparation method for a universal CAR-T cell targeting a T-cell lymphoma cell, the universal CAR-T cell prepared by means of the method and a biological product comprising the universal CAR-T cell. The preparation method for the universal CAR-T cell targeting a T-cell lymphoma cell comprises: obtaining a T cell from a human donor having a healthy lymphatic system, and then disrupting a TRAC genome region and a B2M genome region in the T cell by means of a gene editing technology, so that CAR molecules targeting the T-cell lymphoma cell TCRα/β are stably expressed in the T cell. The universal CAR-T cell targeting a T-cell lymphoma cell prepared by means of the preparation method eliminates the natural TCR expression of a T cell, greatly reduces the graft-versus-host reaction, and meanwhile, also greatly reduces the immunogenicity thereof, and can continuously and efficiently kill the T-cell lymphoma cell.