公开(公告)号：EP3898983A1

公开(公告)日：2021-10-27

申请号：EP19898348.8

申请日：2019-12-20

申请人： Peking University ,  Edigene Biotechnology Inc.

发明人： WEI, Wensheng ,  ZHU, Shiyou ,  CAO, Zhongzheng ,  LIU, Zhiheng ,  HE, Yuan ,  YUAN, Pengfei

IPC分类号： C12N15/66 ,  C12N15/113 ,  C40B40/06 ,  C12N9/22 ,  C12N15/10

公开(公告)号：EP3775205A1

公开(公告)日：2021-02-17

申请号：EP18913494.3

申请日：2018-04-02

申请人： Peking University ,  Edigene Biotechnology Inc.

发明人： WEI, Wensheng ,  LIU, Ying ,  CAO, Zhongzheng ,  WANG, Yinan ,  GUO, Yu ,  YUAN, Pengfei

IPC分类号： C12N15/113 ,  C12N15/86

公开(公告)号：EP4086341A1

公开(公告)日：2022-11-09

申请号：EP20911101.2

申请日：2020-12-30

申请人： Edigene Biotechnology Inc.

发明人： YUAN, Pengfei ,  WANG, Fei ,  NIU, Lichao ,  FU, Jianqiang ,  GU, Feng

IPC分类号： C12N5/10 ,  C12N15/113 ,  C07K16/28 ,  A61K35/17

摘要： A method for purifying a universal human CAR-T cell, comprising: (i) destroying, by using gene editing technology, a TRAC gene region from position 23016448 to position 23016490 of chromosome 14 and a B2M gene region from position 45003745 to position 4500378 of chromosome 15 in the human CAR-T cell; (ii) then introducing a mRNA targeting TCRα/β into the gene-edited CAR-T cell; and (iii) in vitro culturing the CAR-T cell population after the described treatment.

公开(公告)号：EP4086340A1

公开(公告)日：2022-11-09

申请号：EP20910719.2

申请日：2020-12-28

申请人： Edigene Biotechnology Inc.

发明人： WANG, Fei ,  YUAN, Pengfei ,  NIU, Lichao ,  FU, Jianqiang ,  WU, Lanlan

IPC分类号： C12N5/10 ,  C12N15/113

摘要： A novel preparation method for a universal CAR-T cell targeting a T-cell lymphoma cell, the universal CAR-T cell prepared by means of the method and a biological product comprising the universal CAR-T cell. The preparation method for the universal CAR-T cell targeting a T-cell lymphoma cell comprises: obtaining a T cell from a human donor having a healthy lymphatic system, and then disrupting a TRAC genome region and a B2M genome region in the T cell by means of a gene editing technology, so that CAR molecules targeting the T-cell lymphoma cell TCRα/β are stably expressed in the T cell. The universal CAR-T cell targeting a T-cell lymphoma cell prepared by means of the preparation method eliminates the natural TCR expression of a T cell, greatly reduces the graft-versus-host reaction, and meanwhile, also greatly reduces the immunogenicity thereof, and can continuously and efficiently kill the T-cell lymphoma cell.

公开(公告)号：EP3536796A1

公开(公告)日：2019-09-11

申请号：EP17812759.3

申请日：2017-06-16

申请人： Edigene Biotechnology Inc.

发明人： WEI, Wensheng ,  ZHOU, Yuexin ,  ZHANG, Hongmin

IPC分类号： C12N15/90 ,  C12N15/11

摘要： Provided are a donor construct, a gene knockout method, and a system and a kit for gene knockout. In the gene knockout method, a marker gene contained in the donor construct is inserted into a double-strand break position in the cell genome by non-homologous end joining, thus improving the production efficiency of gene knockout by a sequence-specific nuclease through enrichment of gene knockout cells via the expressed marker.