公开(公告)号：EP4433493A1

公开(公告)日：2024-09-25

申请号：EP22818763.9

申请日：2022-11-21

申请人： Enantis s.r.o.

发明人： CHALOUPKOVA, Radka ,  STEPANKOVA, Veronika ,  HORACKOVA, Aneta ,  BEDNAR, David ,  KUTALKOVA, Katerina ,  LASKOVA, Veronika

IPC分类号： C07K14/475

CPC分类号： C07K14/50 ,  C07K2319/2120130101 ,  C07K2319/5020130101 ,  A61K38/00 ,  A61K8/64 ,  A61Q19/00 ,  A61K2800/1020130101

公开(公告)号：EP4183796A1

公开(公告)日：2023-05-24

申请号：EP21209408.0

申请日：2021-11-19

申请人： Enantis s.r.o.

发明人： CHALOUPKOVA, Radka ,  STEPANKOVA, Veronika ,  HORACKOVA, Aneta ,  BEDNAR, David ,  KUTALKOVA, Katerina

IPC分类号： C07K14/475

摘要： The invention relates to a thermostable FGF10 polypeptide possessing FGF10 activity and having or comprising at least 85% sequence identity to SEQ ID NO:3 that has or comprises an amino acid sequence from Ser69 to Ser208 of SEQ ID NO:1, or to SEQ ID NO:5 that has or comprises an amino acid sequence from Leu40 to Ser208 of SEQ ID NO:1, or the fragments thereof, comprising at least an amino acid substitution L152F. Preferably, any of amino acid substitutions V123I, Q175E, and N181D can be further included. The invention further discloses the use of subjected thermostable FGF10 polypeptides in regenerative medicine or other related medical applications or cosmetics. Further, it discloses a culture medium comprising subjected thermostable FGF10 polypeptides suitable for proliferation and differentiation of the human embryonic stem cells or formation and differentiation of spheroids and organoids.