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公开(公告)号：EP3470845B1
公开(公告)日：2020-05-13
申请号：EP18210185.7
申请日：2014-03-18
IPC分类号： G01N33/574 , G01N33/68 , C12N9/10 , C12N9/12
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公开(公告)号：EP3470513B1
公开(公告)日：2020-05-13
申请号：EP18210192.3
申请日：2014-03-18
IPC分类号： C12N9/10 , C12N9/12 , G01N33/574 , G01N33/68
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公开(公告)号：EP3267194B1
公开(公告)日：2019-06-05
申请号：EP17001229.8
申请日：2010-12-22
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公开(公告)号：EP2805346B1
公开(公告)日：2018-05-30
申请号：EP13735986.5
申请日：2013-01-10
CPC分类号： G01N33/74 , C12Q1/37 , G01N33/57488 , G01N33/6845 , G01N33/6848 , G01N2333/72 , G01N2458/15 , G01N2560/00 , H01J49/0027 , H01J49/0031 , H01J49/004 , H01J49/26
摘要： Specific peptides, and derived ionization characteristics of the peptides, from the Insulin Receptor protein (IR), and its isoforms IR-A and IR-B, that are particularly advantageous for quantifying the IR protein, IR-A isoform and/or IR-B isoform, directly in biological samples that have been fixed in formalin by the method of Selected Reaction Monitoring (SRM) mass spectrometry, or what can also be termed as Multiple Reaction Monitoring (MRM) mass spectrometry. Such biological samples are chemically preserved and fixed and are selected from tissues and cells treated with formaldehyde containing agents/fixatives including formalin-fixed tissue/cells, formalin-fixed/paraffin embedded (FFPE) tissue/cells, FFPE tissue blocks and cells from those blocks, and tissue culture cells that have been formalin fixed and or paraffin embedded. A protein sample is prepared from said biological sample using the Liquid Tissue™ reagents and protocol and the IR protein, and IR-A and/or IR-B isoforms, is quantitated in the Liquid Tissue™ sample by the method of SRM/MRM mass spectrometry by quantitating in the protein sample at least one or more of the peptides described. These peptides can be quantitated if they reside in a modified or an unmodified form. An example of a modified form of an IR peptide is phosphorylation of a tyrosine, threonine, serine, and/or other amino acid residues within the peptide sequence.
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公开(公告)号：EP2758779A2
公开(公告)日：2014-07-30
申请号：EP12832855.6
申请日：2012-09-24
IPC分类号： G01N33/53
CPC分类号： G01N33/6848 , G01N33/6863 , G01N2333/70578 , G01N2800/56 , G01N2800/7028
摘要： Specific peptides, and derived ionization characteristics of those peptides from Death Receptor 5 (DR5) protein are provided that are particularly advantageous for quantifying the DR5 protein directly in biological samples that have been fixed in formalin by the method of Selected Reaction Monitoring/Multiple Reaction Monitoring (SRM/MRM) mass spectrometry. Such biological samples are chemically preserved and fixed wherein the biological sample is selected from tissues and cells treated with formaldehyde containing agents/fixatives including formalin-fixed tissue/cells, formalin-fixed/paraffin embedded (FFPE) tissue/cells, FFPE tissue blocks and cells from those blocks, and tissue culture cells that have been formalin fixed and or paraffin embedded. A protein sample is prepared from a biological sample using the Liquid Tissue™ reagents and protocol, and the DR5 protein are quantitated in the Liquid Tissue™ sample by the method of SRM/MRM mass spectrometry by quantitating in the protein sample at least one or more of the peptides described for one or more of the DR5 protein. These peptides can be quantitated if they reside in a modified or in an unmodified form. An example of a modified form of a DR5 peptide is phosphorylation of a tyrosine, threonine, serine, and/or other amino acid residues within the peptide sequence.
摘要翻译： 提供了来自死亡受体5（DR5）蛋白的那些肽的特定肽和衍生离子化特征，其特别有利于通过选择性反应监测/多反应监测方法已经在福尔马林中固定的生物样品中直接定量DR5蛋白 （SRM / MRM）质谱。 这些生物样品是化学保存和固定的，其中生物样品选自用含甲醛的试剂/固定剂包括福尔马林固定的组织/细胞，福尔马林固定的/石蜡包埋的（FFPE）组织/细胞，FFPE组织块和 来自那些块的细胞，以及福尔马林固定和/或石蜡包埋的组织培养细胞。 使用Liquid Tissue TM试剂和方案从生物样品制备蛋白质样品，并且通过SRM / MRM质谱法的方法在Liquid Tissue TM样品中定量DR5蛋白质，其中通过定量蛋白质样品中的至少一种或多种 针对一种或多种DR5蛋白描述的肽。 如果这些肽以修饰形式或未修饰形式存在，则可对其进行定量。 DR5肽的修饰形式的实例是肽序列内的酪氨酸，苏氨酸，丝氨酸和/或其他氨基酸残基的磷酸化
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公开(公告)号：EP2659267A1
公开(公告)日：2013-11-06
申请号：EP11853571.5
申请日：2011-12-27
IPC分类号： G01N30/72 , G01N33/483 , A61K38/18
CPC分类号： G01N33/6872 , G01N33/4833 , G01N33/68 , G01N33/6848 , G01N33/74 , G01N2030/8831 , G01N2333/71 , G01N2333/82 , G01N2560/00 , G01N2800/52 , G01N2800/56
摘要： Specific peptides are provided, and derived ionization characteristics of those peptides, from the Hepatocyte Growth Factor Receptor (cMET) protein. The peptides are particularly and surprisingly advantageous for quantifying by the method of Selected Reaction Monitoring (SRM) mass spectrometry the cMET protein directly in biological samples that have been fixed in formalin, or what can also be termed as Multiple Reaction Monitoring (MRM) mass spectrometry. Such biological samples are chemically preserved and fixed where the biological sample is selected from tissues and cells treated with formaldehyde containing agents/fixatives including: formalin-fixed tissue/cells; formalin-fixed/paraffin embedded (FFPE) tissue/cells; FFPE tissue blocks and cells from those blocks; and tissue culture cells that have been formalin fixed and or paraffin embedded. A protein sample is prepared from the biological sample using the Liquid Tissue™ reagents and protocol and the cMET protein is quantitated in the Liquid Tissue™ sample by the method of SRM/MRM mass spectrometry by quantitating in the protein sample at least one or more of the peptides described. These peptides can be quantitated if they reside in a modified or an unmodified form. An example of a modified form of a cMET peptide is phosphorylation of a tyrosine, threonine, serine, and/or other amino acid residues within the peptide sequence.
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公开(公告)号：EP2658868A1
公开(公告)日：2013-11-06
申请号：EP11852979.1
申请日：2011-12-29
IPC分类号： C07K16/18
CPC分类号： G01N33/6848 , G01N33/574 , G01N33/6893 , G01N33/74 , G01N2333/485 , G01N2333/71 , G01N2333/912 , G01N2333/9121
摘要： The current disclosure provides for specific peptides, and derived ionization characteristics of the peptides, from the Receptor Tyrosine-Protein Kinase erbB-3, or Her3, that are particularly advantageous for quantifying the Her3 protein directly in biological samples that have been fixed in formalin by the method of Selected Reaction Monitoring (SRM) mass spectrometry, or what can also be termed as Multiple Reaction Monitoring (MRM) mass spectrometry. Such biological samples are chemically preserved and fixed wherein said biological sample is selected from tissues and cells treated with formaldehyde containing agents/fixatives including formalin-fixed tissue/cells, formalin-fixed/paraffin embedded (FFPE) tissue/cells, FFPE tissue blocks and cells from those blocks, and tissue culture cells that have been formalin fixed and or paraffin embedded. A protein sample is prepared from said biological sample using the Liquid Tissue™ reagents and protocol and the Her3 protein is quantitated in the Liquid Tissue™ sample by the method of SRM/MRM mass spectrometry by quantitating in the protein sample at least one or more of the peptides described. These peptides can be quantitated if they reside in a modified or an unmodified form. An example of a modified form of a Her3 peptide is phosphorylation of a tyrosine, threonine, serine, and/or other amino acid residues within the peptide sequence.
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公开(公告)号：EP3470845A1
公开(公告)日：2019-04-17
申请号：EP18210185.7
申请日：2014-03-18
IPC分类号： G01N33/574 , G01N33/68 , C12N9/10 , C12N9/12
摘要： The present invention relates to a method for measuring the amount of Ret protein in a human biological sample of formalin-fixed tissue, comprising detecting and quantifying the amount of a Ret protein fragment peptide in a protein digest prepared from said biological sample using mass spectrometry; and calculating the amount of Ret protein in said sample; wherein said Ret protein fragment peptide is the peptide consisting of the sequence of SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 23, SEQ ID NO: 24, SEQ ID NO: 25 or SEQ ID NO: 26; and
wherein said amount is a relative amount or an absolute amount.-
公开(公告)号：EP3470513A1
公开(公告)日：2019-04-17
申请号：EP18210192.3
申请日：2014-03-18
IPC分类号： C12N9/10 , C12N9/12 , G01N33/574 , G01N33/68
摘要： The present invention relates to a method for measuring the amount of Ros protein in a human biological sample of formalin-fixed tissue, comprising detecting and quantifying the amount of an Ros protein fragment peptide in a protein digest prepared from said biological sample using mass spectrometry; and calculating the amount of Ros protein in said sample; wherein said Ros protein fragment peptide is the peptide consisting of the sequence of SEQ ID NO: 6, SEQ ID NO: 7 or SEQ ID NO: 8; and
wherein said amount is a relative amount or an absolute amount.-
公开(公告)号：EP3167293A1
公开(公告)日：2017-05-17
申请号：EP15819454.8
申请日：2015-07-13
IPC分类号： G01N33/68
摘要： The current disclosure provides for specific peptides, and derived ionization characteristics of the peptides, from the GTPase KRas Protein (KRas) that are particularly advantageous for quantifying the KRas protein directly in biological samples that have been fixed in formalin by the mass spectrometry, or what can also be termed as Multiple Reaction Monitoring (MRM) mass spectrometry. Such biological samples are chemically preserved and fixed wherein the biological sample is selected from tissues and cells treated with formaldehyde containing agents/fixatives including formalin fixed tissue/cells, formalin-fixed/paraffin embedded (FFPE) tissue/cells,
摘要翻译： 本公开提供了来自GTPase KRas蛋白（KRas）的肽的特定肽和衍生离子化特征，其特别有利于通过质谱法已经在福尔马林中固定的生物样品中直接定量KRas蛋白，或者 也可以称为多反应监测（MRM）质谱法。 这些生物样品是化学保存和固定的，其中生物样品选自用含甲醛的试剂/固定剂（包括福尔马林固定的组织/细胞，福尔马林固定的/石蜡包埋的（FFPE）组织/细胞，
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