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公开(公告)号：EP0787807A2
公开(公告)日：1997-08-06
申请号：EP97106534.7
申请日：1992-08-19
IPC分类号： C12Q1/70
CPC分类号： C07K14/005 , C12N2770/24222 , C12Q1/707
摘要： The invention provides oligonucleotide probes for detecting hepatitis C virus nucleic acids from the U.S., Japan, and HCV-C prototype strains, as well as oligonucleotide primers for amplifying such nucleic acids. The invention also provides methods and kits for amplifying and detecting said nucleic acids. The amplification methods preferably include the reverse transcription of the viral genomic RNA to create cDNA and the subsequent amplification of the cDNA by the polymerase chain reaction. Oligonucleotide probes can then be used to detect the presence of amplified DNA by hybridization.
摘要翻译： 本发明提供用于检测来自美国，日本和HCV-C原型菌株的丙型肝炎病毒核酸的寡核苷酸探针，以及用于扩增此类核酸的寡核苷酸引物。 本发明还提供用于扩增和检测所述核酸的方法和试剂盒。 扩增方法优选包括病毒基因组RNA的逆转录以产生cDNA并通过聚合酶链反应随后扩增cDNA。 然后可以使用寡核苷酸探针通过杂交来检测扩增的DNA的存在。
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公开(公告)号：EP0787807B1
公开(公告)日：2003-05-28
申请号：EP97106534.7
申请日：1992-08-19
CPC分类号： C07K14/005 , C12N2770/24222 , C12Q1/707
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公开(公告)号：EP0787807A3
公开(公告)日：1998-04-01
申请号：EP97106534.7
申请日：1992-08-19
IPC分类号： C12Q1/70
CPC分类号： C07K14/005 , C12N2770/24222 , C12Q1/707
摘要： The invention provides oligonucleotide probes for detecting hepatitis C virus nucleic acids from the U.S., Japan, and HCV-C prototype strains, as well as oligonucleotide primers for amplifying such nucleic acids. The invention also provides methods and kits for amplifying and detecting said nucleic acids. The amplification methods preferably include the reverse transcription of the viral genomic RNA to create cDNA and the subsequent amplification of the cDNA by the polymerase chain reaction. Oligonucleotide probes can then be used to detect the presence of amplified DNA by hybridization.
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公开(公告)号：EP0529493B1
公开(公告)日：1997-12-17
申请号：EP92114115.6
申请日：1992-08-19
CPC分类号： C07K14/005 , C12N2770/24222 , C12Q1/707
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公开(公告)号：EP0529493A3
公开(公告)日：1993-07-28
申请号：EP92114115.6
申请日：1992-08-19
CPC分类号： C07K14/005 , C12N2770/24222 , C12Q1/707
摘要： The invention provides reagents, methods and kits for amplifying and detecting U.S., Japan, and HCV-C prototype nucleic acids.Oligonucleotides primers can be used to amplify and detect Hepatitis C virus nucleic acids in a process that involves reverse transcription of the viral genomic RNA to create cDNA and the subsequent amplification of the cDNA by the polymerase chain reaction. Oligonucleotide probes can be used to detect the presence of amplified DNA by hybridization.
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6.发明公开
公开(公告)号：EP0529493A2
公开(公告)日：1993-03-03
申请号：EP92114115.6
申请日：1992-08-19
CPC分类号： C07K14/005 , C12N2770/24222 , C12Q1/707
摘要： The invention provides reagents, methods and kits for amplifying and detecting U.S., Japan, and HCV-C prototype nucleic acids.Oligonucleotides primers can be used to amplify and detect Hepatitis C virus nucleic acids in a process that involves reverse transcription of the viral genomic RNA to create cDNA and the subsequent amplification of the cDNA by the polymerase chain reaction. Oligonucleotide probes can be used to detect the presence of amplified DNA by hybridization.
摘要翻译： 本发明提供用于扩增和检测美国，日本和HCV-C原型核酸的试剂，方法和试剂盒。寡核苷酸引物可用于在涉及病毒基因组RNA的逆转录的过程中扩增和检测丙型肝炎病毒核酸 通过聚合酶链反应产生cDNA和随后的cDNA扩增。 寡核苷酸探针可用于通过杂交来检测扩增的DNA的存在。
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