公开(公告)号：EP3121268B1

公开(公告)日：2018-12-26

申请号：EP16179299.9

申请日：2016-07-13

Applicant: Grifols, S.A.

Inventor： BUNO, Brett ,  JOURNIGAN, Terri ,  HOTTA, Joann ,  BURDICK, Michael

IPC: C12N7/00 ,  G01N33/576 ,  C12Q1/70

CPC classification number: C12N7/00 ,  C12N2770/28151 ,  C12Q1/706 ,  C12Q1/707 ,  G01N33/576 ,  G01N33/5768

Abstract: Provided herein is a method of purification of non-enveloped or pseudo-enveloped virus produced in vitro using a composition with at least one detergent. Also provided are a method of purification can use multiple detergents, and a method of determining the presence and/or level of a non-enveloped or pseudo-enveloped virus in a sample.

公开(公告)号：EP3362794A1

公开(公告)日：2018-08-22

申请号：EP16856308.8

申请日：2016-10-14

Applicant: ExThera Medical Corporation

Inventor： WARD, Robert S. ,  MCCREA, Keith R.

IPC: G01N33/543 ,  G01N33/552 ,  G01N33/569

CPC classification number: C12Q1/24 ,  B01D15/3828 ,  C12Q1/70 ,  C12Q1/701 ,  C12Q1/707 ,  C12Q2600/158 ,  G01N33/56905 ,  G01N2333/195 ,  G01N2333/43556 ,  G01N2333/445 ,  G01N2469/10 ,  Y02A50/57 ,  Y02A50/58 ,  Y02A50/60

Abstract: The present invention provides an in vitro method for concentrating infectious pathogens found in a biological sample obtained from an individual who is suspected of being infected with the pathogens. Provided herein is also an in vitro method for reducing or eliminating blood cells from a sample obtained from an individual suspected to being infected with an infectious pathogen. The present invention also provides a method for diagnosing malaria and a method for determining if an individual is infected with a pathogen. Provided herein is also a concentrator and a kit for use with the methods.

公开(公告)号：EP3017068B1

公开(公告)日：2018-04-18

申请号：EP14736385.7

申请日：2014-07-04

Applicant: Fundació Hospital Universitari Vall d'Hebron - Institut de Recerca ,  CIBER (Centro de Investigación Biomédica en Red) ,  Roche Diagnòstics S.L.

Inventor： QUER, Josep ,  RODRÍGUEZ, Francisco ,  GREGORI, Josep

IPC: C12Q1/70

CPC classification number: C12Q1/707 ,  C12Q2600/112 ,  C12Q2600/156 ,  C12Q2600/16

Abstract: The invention relates to an in vitro method for determining in an isolated sample of a subject the presence of one or more Human Hepatitis C Virus (HCV) sequence variants comprising reverse transcription and amplifying HCV RNAs molecules using specific sets of primers allowing the amplification of the HCV regions named 5'-UTR-Core and NS5B. The method includes an step for purifying the amplified regions and a sequencing step of the amplified fragments. The invention also includes specific oligonucleotides than are used as primers in the method, as well as kits including one or more of these oligonucleotides as sets of primers.

公开(公告)号：EP2872649B1

公开(公告)日：2018-02-21

申请号：EP13740457.0

申请日：2013-07-11

Applicant: Gen-Probe Incorporated

Inventor： DATTA, Somalee ,  BOWMAN, Brett ,  WANG, Xianqun ,  WANG, Mingjie

IPC: C12Q1/68 ,  C12Q1/70

CPC classification number: C12Q1/707 ,  C12Q1/6809 ,  C12Q2600/156 ,  C12Q2600/16 ,  G06F19/18 ,  C12Q2535/122 ,  C12Q2537/165

Abstract: Disclosed are methods for detecting a minority genotype of a target nucleic acid. The disclosed method generally includes the steps of (a) deep sequencing at least a portion of the target nucleic acid; (b) using the deep sequencing results of (a) to detect the presence of variant nucleobases at one or more nucleotide reference positions within the target nucleic acid; (c) using the variant detection results generated in step (b) to perform a statistical analysis of whether the variants are significant; and (d) using the variant detection and variant significance results generated in steps (b) and (c) to perform a statistical analysis of whether a subset of sequences together exhibit a common set of significant variants.

公开(公告)号：EP2722398B1

公开(公告)日：2017-07-12

申请号：EP12188990.1

申请日：2012-10-18

Applicant: F. Hoffmann-La Roche AG ,  Roche Diagnostics GmbH

Inventor： Bergmann, Frank ,  Sitzmann, Dorothea ,  Zitzer, Heike

IPC: C12Q1/68 ,  C12Q1/70

CPC classification number: C12Q1/707 ,  C07H21/04 ,  C12Q1/6816 ,  C12Q2537/143

公开(公告)号：EP2839039B1

公开(公告)日：2017-06-07

申请号：EP13716295.4

申请日：2013-04-15

Applicant: F. Hoffmann-La Roche AG ,  Roche Diagnostics GmbH

Inventor： LEYING, Hermann ,  MYERS, Thomas W. ,  NEWTON, Nicolas ,  RUSSMANN, Eberhard ,  SAN FILIPPO, Joseph ,  SCHOENBRUNNER, Nancy ,  WILTS, Heike ,  WU, Xiaoning ,  YOUNG, Karen Kwok Ying ,  ZIMMERMANN, Dirk

IPC: C12Q1/70

CPC classification number: C12Q1/707 ,  C12Q2600/16

公开(公告)号：EP1746171B1

公开(公告)日：2017-05-17

申请号：EP06013306.3

申请日：2006-06-28

Applicant: Roche Diagnostics GmbH ,  F. Hoffmann-La Roche AG

Inventor： Gupta, Amar ,  Will, Stephen Gordon

IPC: C12Q1/68 ,  C12Q1/70

CPC classification number: C12Q1/6818 ,  C12Q1/6823 ,  C12Q1/6851 ,  C12Q1/6858 ,  C12Q1/6883 ,  C12Q1/707 ,  Y10S435/975 ,  C12Q2565/101 ,  C12Q2563/173 ,  C12Q2527/107 ,  C12Q2561/113 ,  C12Q2531/107 ,  C12Q2561/101 ,  C12Q2523/319

公开(公告)号：EP2828382B1

公开(公告)日：2016-10-26

申请号：EP13712475.6

申请日：2013-03-15

Applicant: Hvidovre Hospital ,  Københavns Universitet

Inventor： LI, Yiping ,  SCHEEL, Troels Kasper Høyer ,  ALMEIDA, Santseharay Ramirez ,  GOTTWEIN, Judith M. ,  BUKH, Jens

IPC: C12N7/00 ,  G01N33/576

CPC classification number: C12N7/00 ,  C12N2770/24221 ,  C12N2770/24234 ,  C12N2770/24252 ,  C12N2770/24271 ,  C12Q1/707 ,  C12Q2600/156 ,  C12Q2600/158

公开(公告)号：EP2482846B1

公开(公告)日：2016-06-29

申请号：EP10754407.4

申请日：2010-09-16

Applicant: Hvidovre Hospital ,  The United States Of America, As Represented By The Secretary, Department of Health and Human Services

Inventor： GOTTWEIN, Judith, M. ,  SCHEEL, Troels Kasper Høyer ,  PURCELL, Robert ,  BUKH, Jens

IPC: A61K39/29 ,  C12N7/00

CPC classification number: C12Q1/18 ,  C12N7/00 ,  C12N15/86 ,  C12N2770/24221 ,  C12N2770/24243 ,  C12Q1/707 ,  G01N33/5008

Abstract: The present invention relates to molecular approaches to the production of nucleic acid sequences, which comprises the genome of infectious hepatitis C virus. In particular, invention provides nucleic acid sequences which comprise the genomes of infectious hepatitis C viruses of either genotype 3a (strain S52) or genotype 4a (strain ED43). The invention therefore relates to the use of the nucleic acid sequences and polypeptides encoded by all or part of the sequences in the development of vaccines and diagnostic assays for HCV and in the development of screening assays for the identification of antiviral agents for HCV. The invention therefore also relates to the use of viral particles derived from laboratory animals infected with S52 and ED43 viruses.

公开(公告)号：EP3027740A1

公开(公告)日：2016-06-08

申请号：EP14750437.7

申请日：2014-07-28

Applicant: Hvidovre Hospital

Inventor： ALMEIDA, Santseharay Ramirez ,  LI, Yiping ,  GOTTWEIN, Judith M. ,  BUKH, Jens

IPC: C12N7/00 ,  C07K14/005

CPC classification number: C12N7/00 ,  C07K14/005 ,  C12N2770/24221 ,  C12N2770/24222 ,  C12N2770/24251 ,  C12Q1/707

Abstract: The present invention relates to nucleic acid sequences that encode hepatitis C viruses (HCV) of genotype 2b that are useful in the fundamental research of HCV as well as in the search of a vaccine against HCV. In particular the present invention relates to nucleic acid sequences that comprises HCVs, which are capable of expressing said virus when transfected into cells and are capable of infectivity in vivo.

Abstract translation: 本发明涉及编码基因型2b的丙型肝炎病毒（HCV）的核酸序列，其可用于HCV的基础研究以及针对HCV的疫苗的检索。 特别地，本发明涉及包含HCV的核酸序列，其能够在转染入细胞时能够表达所述病毒并且能够在体内具有感染性。