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    TARGET ENRICHMENT BY UNIDIRECTIONAL DUAL PROBE PRIMER EXTENSION
    5.
    发明公开
    TARGET ENRICHMENT BY UNIDIRECTIONAL DUAL PROBE PRIMER EXTENSION 审中-公开

    公开(公告)号：EP4269582A3

    公开(公告)日：2024-01-24

    申请号：EP23175220.5

    申请日：2018-12-19

    申请人： F. Hoffmann-La Roche AG ,  Roche Diagnostics GmbH ,  Kapa Biosystems, Inc.

    发明人： LOVEJOY, Alexander ,  PENKLER, Jo-Anne Elizabeth ,  GODWIN, Brian Christopher ,  PLATZER, Joseph ,  BURGESS, Daniel ,  MILLER, Bronwen

    IPC分类号： C12N15/10

    摘要： The present disclosure provides a method for enrichment of at least one target nucleic acid in a library of nucleic acids. A first oligonucleotide is hybridized to a target nucleic acid in library of nucleic acids having first and second adapters. The hybridized first oligonucleotide is extended with a first polymerase, thereby producing a first primer extension complex including the target nucleic acid and the extended first oligonucleotide. The first primer extension complex is captured, enriched relative to the library of nucleic acids, and a second oligonucleotide is hybridized to the target nucleic acid. The hybridized second oligonucleotide is extended with a second polymerase, thereby producing a second primer extension complex including the target nucleic acid and the extended second oligonucleotide, and further liberating the extended first oligonucleotide from the first primer extension complex.

    TARGET ENRICHMENT BY UNIDIRECTIONAL DUAL PROBE PRIMER EXTENSION
    6.
    发明公开
    TARGET ENRICHMENT BY UNIDIRECTIONAL DUAL PROBE PRIMER EXTENSION 审中-公开

    公开(公告)号：EP4269582A2

    公开(公告)日：2023-11-01

    申请号：EP23175220.5

    申请日：2018-12-19

    申请人： F. Hoffmann-La Roche AG ,  Roche Diagnostics GmbH ,  Kapa Biosystems, Inc.

    发明人： LOVEJOY, Alexander ,  PENKLER, Jo-Anne Elizabeth ,  GODWIN, Brian Christopher ,  PLATZER, Joseph ,  BURGESS, Daniel ,  MILLER, Bronwen

    IPC分类号： C12N15/10

    摘要： The present disclosure provides a method for enrichment of at least one target nucleic acid in a library of nucleic acids. A first oligonucleotide is hybridized to a target nucleic acid in library of nucleic acids having first and second adapters. The hybridized first oligonucleotide is extended with a first polymerase, thereby producing a first primer extension complex including the target nucleic acid and the extended first oligonucleotide. The first primer extension complex is captured, enriched relative to the library of nucleic acids, and a second oligonucleotide is hybridized to the target nucleic acid. The hybridized second oligonucleotide is extended with a second polymerase, thereby producing a second primer extension complex including the target nucleic acid and the extended second oligonucleotide, and further liberating the extended first oligonucleotide from the first primer extension complex.