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    SlpA as a tool for recombinant protein and enzyme technology
    1.
    发明公开
    SlpA as a tool for recombinant protein and enzyme technology 审中-公开
    SlpA als Werkzeugfürrecombinante Protein-und Enzym-technologie

    公开(公告)号：EP2127678A1

    公开(公告)日：2009-12-02

    申请号：EP08009537.5

    申请日：2008-05-26

    申请人： Roche Diagnostics GmbH ,  F.HOFFMANN-LA ROCHE AG

    发明人： Scholz, Christian, Dr. ,  Faatz, Elke, Dr., Dr. ,  Schaarschmidt, Peter, Dr. ,  Schmitt, Urban

    IPC分类号： A61K47/48 ,  C07K14/16 ,  C12N9/90 ,  G01N33/543 ,  G01N33/569

    CPC分类号： C12N15/62 ,  C12N9/90 ,  G01N33/536 ,  G01N33/54393 ,  Y02A50/57

    摘要： The present invention relates to a recombinant DNA molecule encoding a fusion protein comprising a SlpA chaperone and a target polypeptide, a corresponding expression vector encoding said fusion protein as well as host cells transformed with said expression vector. Another aspect of the invention is a method for producing said fusion protein as well as a recombinantly produced fusion protein comprising a SlpA chaperone and a target polypeptide. A further aspect of the invention is the use of the recombinantly produced fusion protein as a binding partner of as a means for the reduction of interferences in an immunoassay. Further the invention relates to the use of the recombinantly produced fusion protein for immunization of laboratory animals in order to produce antibodies and to the use of the recombinantly produced fusion protein in the production of a vaccine. Yet another aspect is a method for the detection of an analyte in an immunoassay using a recombinantly produced fusion protein as well as a reagent kit containing a recombinantly produced fusion protein comprising a SlpA chaperone and a target polypeptide. A further aspect of the invention concerns the use of SlpA for the reduction of interferences in an immunoassay and its use as an additive in protein formulations and as a folding helper in biotechnological applications.

    摘要翻译： 本发明涉及编码包含SlpA伴侣和靶多肽的融合蛋白的重组DNA分子，编码所述融合蛋白的相应表达载体以及用所述表达载体转化的宿主细胞。 本发明的另一方面是生产所述融合蛋白的方法以及重组产生的包含SlpA伴侣和靶多肽的融合蛋白。 本发明的另一方面是使用重组产生的融合蛋白作为结合配偶体作为减少免疫测定中的干扰的手段。 此外，本发明涉及重组产生的融合蛋白用于免疫实验室动物以产生抗体的用途，以及在疫苗生产中使用重组产生的融合蛋白。 另一方面是使用重组产生的融合蛋白在免疫测定中检测分析物的方法以及含有重组产生的包含SlpA伴侣和靶多肽的融合蛋白的试剂盒。 本发明的另一方面涉及SlpA用于减少免疫测定中的干扰及其在蛋白质制剂中作为添加剂的用途以及在生物技术应用中作为折叠辅助剂的用途。