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    METHOD FOR IDENTIFICATION AND DETECTION OF MUTANT GENE USING INTERCALATOR
    1.
    发明授权
    METHOD FOR IDENTIFICATION AND DETECTION OF MUTANT GENE USING INTERCALATOR 有权

    公开(公告)号：EP2840137B1

    公开(公告)日：2018-07-25

    申请号：EP13764366.4

    申请日：2013-03-22

    申请人： FUJIFILM Wako Pure Chemical Corporation ,  Kanagawa Prefectural Hospital Organization

    发明人： MATSUKUMA, Shoichi ,  ISHIKAWA, Tomokazu ,  KUROSAWA, Tatsuo

    IPC分类号： C12Q1/6827

    CPC分类号： C12Q1/6823 ,  C12Q1/6827 ,  G01N2458/30 ,  C12Q2563/173 ,  C12Q2565/125 ,  C12Q2565/131 ,  C12Q2565/133

    摘要： The present invention addresses the problem of improving separation performance in separating a hybrid of a mutant-type DNA and a probe or/and a hybrid of a wild-type type DNA and a probe, said hybrids being obtained by the LH method. The present invention relates to "a method for detection of mutant-type DNA or/and wild-type DNA which comprises the following steps: (1) a step in which at least one of a single-stranded DNA having a substituted nucleotide, a deficient nucleotide region, or an inserted nucleotide region (mutant-type DNA), or/and a wild-type single-stranded DNA corresponding to the mutant-type DNA (wild-type DNA) is contacted with a probe hybridizing with both single-stranded DNAs, to form a hybrid with the mutant-type DNA (mutant-type hybrid) or/and a hybrid with the wild-type DNA (wild-type hybrid) (in this regard however, at least one of the obtained mutant-type hybrid and wild-type hybrid has a loop structure), (2) a step in which the obtained mutant-type hybrid or/and wild-type hybrid is contacted with an intercalator, and (3) a step in which the presence or absence of the mutant-type DNA or/and the wild-type DNA is detected by separating the conjugate of mutant-type hybrid and intercalator or/and the conjugate of wild-type hybrid and intercalator".

    METHOD FOR IDENTIFICATION AND DETECTION OF MUTANT GENE USING INTERCALATOR
    3.
    发明公开
    METHOD FOR IDENTIFICATION AND DETECTION OF MUTANT GENE USING INTERCALATOR 有权
    ERKENNUNG MUTIERENDER基因遗传病患者的VERFAHREN ZUR鉴定EINEM INTERKALATOR

    公开(公告)号：EP2840137A1

    公开(公告)日：2015-02-25

    申请号：EP13764366.4

    申请日：2013-03-22

    申请人： Wako Pure Chemical Industries, Ltd. ,  Kanagawa Prefectural Hospital Organization

    发明人： MATSUKUMA, Shoichi ,  ISHIKAWA, Tomokazu ,  KUROSAWA, Tatsuo

    IPC分类号： C12N15/09 ,  C12Q1/68

    CPC分类号： C12Q1/6823 ,  C12Q1/6827 ,  G01N2458/30 ,  C12Q2563/173 ,  C12Q2565/125 ,  C12Q2565/131 ,  C12Q2565/133

    摘要： The present invention addresses the problem of improving separation performance in separating a hybrid of a mutant-type DNA and a probe or/and a hybrid of a wild-type type DNA and a probe, said hybrids being obtained by the LH method.
    The present invention relates to "a method for detection of mutant-type DNA or/and wild-type DNA which comprises the following steps: (1) a step in which at least one of a single-stranded DNA having a substituted nucleotide, a deficient nucleotide region, or an inserted nucleotide region (mutant-type DNA), or/and a wild-type single-stranded DNA corresponding to the mutant-type DNA (wild-type DNA) is contacted with a probe hybridizing with both single-stranded DNAs, to form a hybrid with the mutant-type DNA (mutant-type hybrid) or/and a hybrid with the wild-type DNA (wild-type hybrid) (in this regard however, at least one of the obtained mutant-type hybrid and wild-type hybrid has a loop structure), (2) a step in which the obtained mutant-type hybrid or/and wild-type hybrid is contacted with an intercalator, and (3) a step in which the presence or absence of the mutant-type DNA or/and the wild-type DNA is detected by separating the conjugate of mutant-type hybrid and intercalator or/and the conjugate of wild-type hybrid and intercalator".

    摘要翻译： 本发明解决了通过LH法获得的突变型DNA与探针或/和野生型型DNA与探针杂交体的杂交体分离性能提高的问题。 本发明涉及“突变型DNA或/和野生型DNA的检测方法”，其包括以下步骤：（1）将具有取代核苷酸的单链DNA， 与突变型DNA（野生型DNA）相对应的野生型单链DNA（或野生型DNA）与插入的核苷酸区域（突变型DNA）或插入的核苷酸区域（突变型DNA） 与突变型DNA（突变型杂合体）或/和野生型DNA（野生型杂种）的杂交体形成杂交体（然而，在这方面，至少一种获得的突变型DNA） 型杂合体和野生型杂合体具有环结构），（2）所获得的突变型杂合体或/和野生型杂合体与嵌入剂接触的步骤，和（3） 突变型DNA或/和野生型DNA的缺失通过分离突变型杂合体和嵌入剂的共轭物或/和 野生型杂种和嵌入剂的结合物“。

    METHOD FOR DETECTING DNA HAVING MICROSATELLITE REGION
    4.
    发明公开
    METHOD FOR DETECTING DNA HAVING MICROSATELLITE REGION 有权
    法DNA与小卫星区域检测

    公开(公告)号：EP2840136A1

    公开(公告)日：2015-02-25

    申请号：EP13764078.5

    申请日：2013-03-22

    申请人： Wako Pure Chemical Industries, Ltd. ,  Kanagawa Prefectural Hospital Organization

    发明人： MATSUKUMA, Shoichi ,  ISHIKAWA, Tomokazu ,  KUROSAWA, Tatsuo

    IPC分类号： C12N15/09 ,  C12Q1/68

    CPC分类号： C12Q1/6876 ,  C12Q2600/156

    摘要： The problem of the present invention is to provide a method which DNA having a microsatellite region can be accurately detected without causing the problem of non-specific reaction product.
    The present invention relates to: "a method for detecting DNA having a microsatellite region by (1) contacting a probe, which does not have a nucleotide sequence complementary to said microsatellite region and hybridizes with both sides of nucleotide sequences of said microsatellite region, with DNA having the microsatellite region, to form a hybrid of the above-mentioned DNA and the above-mentioned probe, which has a loop structure including microsatellite region, (2) separating the obtained hybrid, (3) detecting said hybrid" and "a hybrid of DNA and a probe, having a loop structure including the microsatellite region, which is made by contacting DNA having microsatellite region with the probe which does not have the nucleotide sequence complementary to said microsatellite region, and hybridizes with both sides of nucleotide sequence of said microsatellite region".