公开(公告)号：EP3796001A1

公开(公告)日：2021-03-24

申请号：EP19804276.4

申请日：2019-05-17

申请人： FUJIFILM Wako Pure Chemical Corporation ,  HIROSAKI UNIVERSITY

发明人： OHYAMA, Chikara ,  YONEYAMA, Tohru ,  TOBISAWA, Yuki ,  ISHIKAWA, Tomokazu ,  DATE, Mutsuhiro ,  NAKAMURA, Kenji

IPC分类号： G01N33/574 ,  C07K14/42 ,  C07K16/18 ,  C12N15/09

摘要： An object of the present invention is to provide a novel determination method which is capable of determining prostate carcinoma with a higher biopsy avoidance rate.
The present invention relates to a method for determining prostate carcinoma, including obtaining a ratio 1 between an amount of a free prostate specific antigen and an amount of a free PSA having an α(2,3) glycan, the free PSA having an α(2,3) glycan being a free prostate specific antigen having a glycan in which a terminal sialic acid residue of the glycan is α(2,3)-linked to a second galactose residue from a terminal of the glycan, in a sample derived from a subject; obtaining a ratio 2 between the ratio 1 and a volume of the prostate of the subject; and determining prostate carcinoma based on the obtained ratio 2.

公开(公告)号：EP3410118A1

公开(公告)日：2018-12-05

申请号：EP16888162.1

申请日：2016-12-08

申请人： FUJIFILM Wako Pure Chemical Corporation ,  Hirosaki University

发明人： OYAMA, Chikara ,  YONEYAMA, Tohru ,  TOBISAWA, Yuki ,  ISHIKAWA, Tomokazu ,  KUROSAWA, Tatsuo ,  NAKAMURA, Kenji

IPC分类号： G01N33/574 ,  C12Q1/68 ,  G01N27/447 ,  G01N37/00

摘要： An object of the present invention is to provide a novel Pca determination method and a Pca malignancy determination method. The present invention is an invention relating to a "prostate carcinoma determination method, including determining the ratio of the amount of free prostate specific antigen, which has a glycan in which the terminal sialic acid residue of the glycan is α(2,3)-linked to a second galactose residue from the terminal of the glycan, to the amount of free PSA in a biological sample, and then determining that prostate carcinoma is developed or the probability of developing prostate carcinoma is high in the case where the ratio is 40% or higher."

公开(公告)号：EP3410118B1

公开(公告)日：2020-09-30

申请号：EP16888162.1

申请日：2016-12-08

申请人： FUJIFILM Wako Pure Chemical Corporation ,  HIROSAKI UNIVERSITY

发明人： OHYAMA, Chikara ,  YONEYAMA, Tohru ,  TOBISAWA, Yuki ,  ISHIKAWA, Tomokazu ,  KUROSAWA, Tatsuo ,  NAKAMURA, Kenji

IPC分类号： G01N33/574 ,  C12Q1/68 ,  G01N27/447 ,  G01N37/00

公开(公告)号：EP2840137B1

公开(公告)日：2018-07-25

申请号：EP13764366.4

申请日：2013-03-22

申请人： FUJIFILM Wako Pure Chemical Corporation ,  Kanagawa Prefectural Hospital Organization

发明人： MATSUKUMA, Shoichi ,  ISHIKAWA, Tomokazu ,  KUROSAWA, Tatsuo

IPC分类号： C12Q1/6827

CPC分类号： C12Q1/6823 ,  C12Q1/6827 ,  G01N2458/30 ,  C12Q2563/173 ,  C12Q2565/125 ,  C12Q2565/131 ,  C12Q2565/133

摘要： The present invention addresses the problem of improving separation performance in separating a hybrid of a mutant-type DNA and a probe or/and a hybrid of a wild-type type DNA and a probe, said hybrids being obtained by the LH method. The present invention relates to "a method for detection of mutant-type DNA or/and wild-type DNA which comprises the following steps: (1) a step in which at least one of a single-stranded DNA having a substituted nucleotide, a deficient nucleotide region, or an inserted nucleotide region (mutant-type DNA), or/and a wild-type single-stranded DNA corresponding to the mutant-type DNA (wild-type DNA) is contacted with a probe hybridizing with both single-stranded DNAs, to form a hybrid with the mutant-type DNA (mutant-type hybrid) or/and a hybrid with the wild-type DNA (wild-type hybrid) (in this regard however, at least one of the obtained mutant-type hybrid and wild-type hybrid has a loop structure), (2) a step in which the obtained mutant-type hybrid or/and wild-type hybrid is contacted with an intercalator, and (3) a step in which the presence or absence of the mutant-type DNA or/and the wild-type DNA is detected by separating the conjugate of mutant-type hybrid and intercalator or/and the conjugate of wild-type hybrid and intercalator".