公开(公告)号：EP1365021A1

公开(公告)日：2003-11-26

申请号：EP02700571.9

申请日：2002-02-08

申请人： FUJISAWA PHARMACEUTICAL CO., LTD.

发明人： MIYOSHI, S., c/o Fujisawa Pharmaceutical Co., Ltd. ,  OHKUBO, A., c/o Fujisawa Pharmaceutical Co., Ltd. ,  MORIKAWA, N., c/o Fujisawa Pharmaceutical Co., Ltd ,  OGAWA, Y., c/o Fujisawa Pharmaceutical Co., Ltd. ,  NISHIMURA,S., c/o Fujisawa Pharmaceutical Co., Ltd ,  FUKAGAWA, M., c/o Fujisawa Pharmaceutical Co., Ltd ,  ARAKAWA, H., c/o Fujisawa Pharmaceutical Co.,Ltd. ,  ZENKOU, Jyunko ,  SATO,Susumu, c/o Fujisawa Pharmaceutical Co., Ltd.

IPC分类号： C12N15/02 ,  C12N15/09 ,  C12M1/00 ,  C12M1/42

CPC分类号： C12N15/87 ,  C12M35/02 ,  C12N15/02

摘要： A method of highly efficiently transferring various selected molecules into various cells and a method of fusing cells. Cells and/or selected molecules such as polynucleotide are treated with cold gas plasma to thereby transfer the selected molecules located around cells into the cells, or cells are fused by treating the cells with cold gas plasma. Moreover, an apparatus for transferring selected molecules or fusing cells having a cold gas plasma generation unit for transferring selected molecules into cells is provided.

摘要翻译： 将各种选择的分子高效转移到各种细胞中的方法以及融合细胞的方法。 用冷气体等离子体处理细胞和/或选择的分子，例如多核苷酸，从而将位于细胞周围的选定分子转移到细胞中，或者通过用冷气体等离子体处理细胞来融合细胞。 此外，提供了用于转移所选择的分子或具有用于将选定分子转移到细胞中的具有冷气体等离子体产生单元的融合电池的装置。