公开(公告)号：EP4423235A1

公开(公告)日：2024-09-04

申请号：EP22800822.3

申请日：2022-10-25

Applicant: Nanocav, LLC

Inventor： CHANG, Chihwei ,  RICHARDSON, Wade ,  MEHRABI, Artin

IPC: C12M3/06 ,  B01L3/00 ,  C12M1/42

CPC classification number: B01L3/502761 ,  C12M23/16 ,  C12M35/02 ,  B01L2400/041520130101 ,  B01L2300/088320130101

公开(公告)号：EP2940121B1

公开(公告)日：2018-08-01

申请号：EP14191272.5

申请日：2014-10-31

Applicant: Lonza Cologne GmbH

Inventor： Altrogge, Ludger ,  Gleissner, Timo ,  Heinze, Andreas ,  Hermsmeier, Sven

IPC: A61N1/04 ,  C12N13/00 ,  C12M1/42 ,  C12N15/87

CPC classification number: C12N13/00 ,  A61N1/0412 ,  C12M35/02 ,  C12N15/87

Abstract: The invention relates to a device for applying an electric field to a suspension of cells, comprising at least one chamber which comprises at least one internal space 40 for holding the suspension, the internal space 40 comprising at least two segments 41, 42, wherein each segment 41, 42 comprises at least one electrode 43, 44 and wherein neighboring electrodes 43, 44 are separated from each other by at least one gap 47 which is at least partially filled with an insulating material 46, and wherein the edges of the electrodes 43, 44 facing each other within the internal space 40 are rounded. Rounding the electrodes' edges facing a neighboring electrode results in a significant reduction of field gradients and thus even of the risk of arcing. The invention further concerns a method, wherein voltage is applied to at least one active electrode 43, 44 while the electrodes 43, 44, 45 or electrode segments next and/or opposite to the active electrode 43, 44 are set to ground potential. Setting neighboring electrodes that surround the active electrode to ground potential results in decreased scattering of the electric field within the internal space so that the electrically active area is locally limited and the field lines are focused near the active electrode and thus control of the process is enhanced.

公开(公告)号：EP3138920B1

公开(公告)日：2018-06-13

申请号：EP16184648.0

申请日：2016-08-18

Applicant: Miltenyi Biotec GmbH

Inventor： KABAHA, Eiad ,  BODDENBERG, Jan ,  MILTENYI, Stefan ,  PETERS, Ralf-Peter

IPC: C12N15/87 ,  C12M1/42

CPC classification number: C12M35/02 ,  A61N1/327 ,  C12M23/28 ,  C12N13/00 ,  C12N15/87

Abstract: The invention is directed to disposable for electroporation of cells, comprising a fluid compartment in an interior of the disposable; a first fluid port for providing cell suspension to the fluid compartment, and a second fluid port for delivering a fluid comprising at least one compound to be electroporated into the cells to the fluid compartment; a first electrode and a second electrode disposed in the fluid compartment; at least one exit port which delivers the fluid from the fluid compartment wherein the first and second fluid port have a fluid communication to a mixing channel which has a fluid communication to the fluid compartment.

公开(公告)号：EP3149147A4

公开(公告)日：2018-05-02

申请号：EP15800086

申请日：2015-05-29

Applicant: ICAHN SCHOOL MED MOUNT SINAI

Inventor： COSTA KEVIN DAVID ,  CASHMAN TIMOTHY JAMES ,  BACKERIS PETER CONSTANTINE

IPC: C12M3/00 ,  C12N5/00

CPC classification number: C12M21/08 ,  C12M23/00 ,  C12M23/26 ,  C12M23/38 ,  C12M35/02

Abstract: A bioreactor system for preparing a cardiac organoid chamber and for subsequent testing thereof is described herein and shown in the exemplary drawing figures. The bioreactor system includes a first vessel having a hollow interior and an open top. A first cover is mated with the open top of the first vessel. The first cover has a first opening formed therein. The system further includes a cannula having a lumen that extends from an open first end to an open second end. The cannula is disposed within the first opening of the first cover such that a portion of the cannula lies below the first cover and for insertion into the hollow interior of the first vessel. A porous ring is coupled to the cannula at or proximate the open second end thereof. The system also includes a balloon catheter having an inflatable balloon at a distal end of a catheter shaft. The balloon catheter is adapted to pass through the lumen of the cannula when the balloon is in a deflated state. The balloon catheter is axially adjustable within the lumen to allow the balloon in an inflated state to be disposed adjacent: (1) the open second end of the cannula; and (2) the porous ring for preparing the cardiac organoid chamber about the inflated balloon and porous ring. The cannula and porous ring construction and combination allows for the balloon to be deflated and removed from the lumen of the cannula while the engineered cardiac organoid chamber remains attached to the porous ring. This permits the testing of organoid pump function, such as organoid pressure and volume characteristics, without having to transfer the engineered cardiac organoid from one tool (e.g., an incubation tool) to another tool (e.g., a functional testing apparatus).

公开(公告)号：EP3260163A1

公开(公告)日：2017-12-27

申请号：EP17184306.3

申请日：2014-05-02

Applicant: Lonza Cologne GmbH

Inventor： Altrogge, Ludger ,  Gleissner, Timo ,  Heinze, Andreas ,  Hermsmeier, Sven

IPC: A61N1/04 ,  A61N1/30 ,  C12M1/42 ,  C12N13/00 ,  C12N15/87 ,  G01N35/10

CPC classification number: C12N13/00 ,  A61N1/0412 ,  C12M35/02 ,  C12N15/87

Abstract: The invention relates to a device 1 for applying an electric field to a suspension of cells, cell derivatives, organelles, sub-cellular particles and/or vesicles, comprising at least one chamber 6 which comprises at least two segments, each segment comprising at least one electrode 4, 5, wherein at least one port 7, 8 is disposed at one end 9 of the chamber 6 and at least one further port 10, 11 is disposed at the opposite end 12 of the chamber 6. According to the invention each segment is provided with at least one first electrode 4 and at least one second electrode 5, wherein the second electrode 5 is a common electrode of at least two segments. In order to avoid arcing and/or undesired heating of the suspension, voltage pulses can be applied to different segments of the chamber sequentially.

Abstract translation: 本发明涉及一种用于向细胞，细胞衍生物，细胞器，亚细胞颗粒和/或囊泡的悬浮液施加电场的装置1，其包括至少一个腔室6，腔室6包括至少两个区段，每个区段至少包括 一个电极4,5，其中至少一个端口7,8设置在腔室6的一个端部9处，并且至少一个另外的端口10,11设置在腔室6的相对端部12处。根据本发明，每个 段设置有至少一个第一电极4和至少一个第二电极5，其中第二电极5是至少两段的公共电极。 为了避免悬浮的电弧和/或不希望的加热，可以将电压脉冲顺序地施加到腔室的不同区段。

公开(公告)号：EP3124595A4

公开(公告)日：2017-11-15

申请号：EP14887546

申请日：2014-04-11

Applicant: GUANGZHOU INST ADVANCED TECHNOLOGY CAS

Inventor： CUI JINMING ,  ZENG SHIJIE ,  EICHSTAEDT OLAF ,  HUANG JIANDONG ,  DU RUXU ,  YUAN HAI

IPC: C12M3/00

CPC classification number: C12M41/46 ,  C12M23/34 ,  C12M23/40 ,  C12M23/42 ,  C12M29/04 ,  C12M35/02 ,  C12M41/36

Abstract: The invention discloses a cell culture and experiment device used in the field of biological and genetic engineering experiment apparatus, comprising a central distribution compartment, a culture compartment, a treatment compartment, and pipelines for delivering liquid between the central distribution compartment and the culture compartment and between the central distribution compartment and the treatment compartment. The central distribution compartment is equipped with a distribution chamber and a piston which can be moved forward and backward in the distribution chamber to alter the working volume of the distribution chamber. At the bottom of the distribution chamber, the central distribution compartment is equipped with a distribution valve controlling the connectivity between the distribution chamber and any of the channels. The invention provides a miniaturized apparatus integrating the central distribution compartment, the culture compartment and the treatment compartment, which can replace manual operations, save time and labor, and avoid wasting experimental raw material.

公开(公告)号：EP2459696B1

公开(公告)日：2017-11-08

申请号：EP10805905.6

申请日：2010-07-30

Applicant: Qvella Corporation

Inventor： TALEBOUR, Samad ,  KHINE, Aye Aye ,  ALAVIE, Tino ,  LEONARD, Stephan W ,  MAASKANT, Robert

IPC: C12M1/42 ,  C12M1/33 ,  C12M1/34 ,  C12Q1/02 ,  C12Q1/68 ,  G01N27/26 ,  G01N33/50 ,  G01N33/543 ,  C12N11/00 ,  C12N13/00

CPC classification number: G01N33/5438 ,  B01L3/502715 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/0645 ,  B01L2300/0809 ,  C12M23/16 ,  C12M25/00 ,  C12M25/04 ,  C12M29/10 ,  C12M35/02 ,  C12M41/38 ,  C12N1/066 ,  C12N11/14 ,  C12N13/00 ,  G01N33/5005

Abstract: The present invention provides a microfluidic devices and methods of use thereof for the concentration and capture of cells. A pulsed non-Faradaic electric field is applied relative to a sample under laminar flow, which results to the concentration and capture of charged analyte. Advantageously, pulse timing is selected to avoid problems associated with ionic screening within the channel. At least one of the electrodes within the channel is coated with an insulating layer to prevent a Faradaic current from flowing in the channel. Under pulsed application of a unipolar voltage to the electrodes, charged analyte within the sample is moved towards one of the electrodes via a transient electrophoretic force.

公开(公告)号：EP3070157A4

公开(公告)日：2017-05-31

申请号：EP14859715

申请日：2014-10-10

Applicant: HAMAMATSU PHOTONICS KK

Inventor： ITO TAIRA ,  TAKAHASHI AKIRA

IPC: C12M1/42 ,  C12M1/32 ,  C12M1/34 ,  G01N33/483 ,  G01N33/487

CPC classification number: G01N33/4836 ,  C12M23/12 ,  C12M35/02 ,  C12M41/36 ,  C12M41/46 ,  G01N33/48728

Abstract: A cell observation apparatus is a cell observation apparatus for observing a cell held by a microplate having an array of wells holding respective samples, which comprises a microplate holder for the microplate to be mounted thereon, and an electrical stimulation unit in which a plurality of electrode pairs, each electrode pair including a negative electrode and a positive electrode, are arranged, wherein the negative electrode and the positive electrode are coated with respective insulators except for their leading ends facing the well, and the leading ends form respective electrode portions exposed to the outside, and wherein a space penetrating upward from the electrode portions is formed between the negative electrode and the positive electrode.

Abstract translation: 细胞观察装置是一种细胞观察装置，用于观察由具有保持相应样品的孔阵列的微孔板保持的细胞，所述微孔板包括用于待安装在其上的微板的微板夹持器以及电刺激单元，其中多个电极 每个电极对包括负电极和正电极，其中负电极和正电极除了它们的前端面向阱之外被涂覆有相应的绝缘体，并且引导端形成暴露于 外部，并且其中从所述电极部分向上穿透的空间形成在所述负极和所述正极之间。

公开(公告)号：EP3168290A1

公开(公告)日：2017-05-17

申请号：EP15818684.1

申请日：2015-06-30

Applicant: Logos Biosystems, Inc.

Inventor： CHO, Keun Chang ,  JUNG, Neon Cheol

IPC: C12M1/42 ,  C12M1/00

CPC classification number: C12M35/02 ,  C12M1/00 ,  C12M1/42 ,  C12M25/06 ,  G01N27/44704 ,  G01N27/453

Abstract: An apparatus for clearing tissue using electrophoresis according to the present invention relates to an apparatus used for separating constituents of biological tissue and clearing the tissue, the apparatus comprising: a chamber which can contain therein a buffer solution and the biological tissue and has an inlet port and an outlet port for circulating the buffer solution; a support member, located within the chamber, for supporting the biological tissue; and electrodes located within the chamber and formed separately of a first electrode and a second electrode which correspond to each other. According to the present invention, since lower voltage is applied at the same current by using a plate electrode instead of an existing wire electrode, such that the amount of side reaction is small and a rapid rise in the temperature of the buffer solution is prevented, and thereby the circulation speed of the buffer solution through the inlet port and the outlet port is slowed down, it is possible to minimize the degradation of decomposition efficiency and quickly clear the tissue compared to a conventional apparatus.

Abstract translation: 根据本发明的用于使用电泳的组织清除设备涉及一种用于分离生物组织的组成成分并清除组织的设备，该设备包括：腔室，其中可以包含缓冲溶液和生物组织，并且具有入口端口 以及用于循环缓冲溶液的出口端口; 位于腔室内的用于支撑生物组织的支撑构件; 以及位于腔室内并且分别由彼此对应的第一电极和第二电极形成的电极。 根据本发明，由于使用板电极代替现有的电极丝以相同的电流施加较低的电压，使得副反应的量小，并且防止了缓冲溶液的温度的快速升高， 从而缓冲溶液通过入口和出口的循环速度减慢，与常规设备相比，可以使分解效率的降低最小化并且快速清除组织。

公开(公告)号：EP3164482A1

公开(公告)日：2017-05-10

申请号：EP14892233.9

申请日：2014-12-10

Applicant: Massachusetts Institute of Technology

Inventor： BUIE, Cullen, Richard ,  DOMINGUEZ, Paulo Andres, Garcia ,  GE, Zhifei ,  MORAN, Jeffrey, Lawrence

IPC: C12M3/06 ,  C12M1/26 ,  C12M1/42

CPC classification number: C12N13/00 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2200/10 ,  B01L2300/0645 ,  B01L2300/0877 ,  B01L2300/12 ,  C12M23/16 ,  C12M33/00 ,  C12M35/02

Abstract: An electroporation device with a volume of varying cross sectional area that as a fast assay device for determining the optimal conditions for plasma membrane electroporation.

Abstract translation: 具有不同横截面积体积的电穿孔装置作为用于确定质膜电穿孔的最佳条件的快速测定装置。