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公开(公告)号：EP2136921B1
公开(公告)日：2012-08-29
申请号：EP08735283.7
申请日：2008-04-16
申请人： Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. , Institut Für Bioprozess- und Analysenmesstechnik e.V.
CPC分类号： B01F13/0071 , B01F13/0076 , B01L1/025 , B01L3/502792 , B01L2200/06 , B01L2200/0647 , B01L2300/089 , B01L2400/0406 , B01L2400/0424 , B01L2400/0427 , B01L2400/0457 , C12M25/01
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公开(公告)号：EP2136921B8
公开(公告)日：2012-10-24
申请号：EP08735283.7
申请日：2008-04-16
申请人： Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. , Institut für Bioprozess- und Analysenmesstechnik e.V.
CPC分类号： B01F13/0071 , B01F13/0076 , B01L1/025 , B01L3/502792 , B01L2200/06 , B01L2200/0647 , B01L2300/089 , B01L2400/0406 , B01L2400/0424 , B01L2400/0427 , B01L2400/0457 , C12M25/01
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公开(公告)号：EP2136921A1
公开(公告)日：2009-12-30
申请号：EP08735283.7
申请日：2008-04-16
申请人： Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. , Institut Für Bioprozess- und Analysentechnik E.V.
CPC分类号： B01F13/0071 , B01F13/0076 , B01L1/025 , B01L3/502792 , B01L2200/06 , B01L2200/0647 , B01L2300/089 , B01L2400/0406 , B01L2400/0424 , B01L2400/0427 , B01L2400/0457 , C12M25/01
摘要： The invention relates to a method for drop manipulation, comprising the steps: preparation of at least one drop (1, 2, 3,...) in contact with a rigid retainer surface (21) of a drop holder (20), generation of an electrical field at the retainer surface (21), wherein a force is exerted on the at least one drop (1, 2, 3,...) and displacement of the at least one drop (1, 2, 3,...) by means of the action of the force, wherein the retainer surface (21) is arranged such that a surface normal to the retainer surface (21) forms an angle α to the vertical direction of gravity of less than or equal to 90° and the drop hangs from the retainer surface (21). A drop manipulation device for the manipulation of hanging drops is also disclosed.
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公开(公告)号：EP1425384B1
公开(公告)日：2006-12-20
申请号：EP02776674.0
申请日：2002-09-13
申请人： HANS-KNÖLL-INSTITUT FÜR NATURSTOFF-FORSCHUNG E.V. , INSTITUT FÜR BIOPROZESS- UND ANALYSENMESSTECHNIK E.V. , INSTITUT FÜR PHYSIKALISCHE HOCHTECHNOLOGIE E.V.
发明人： MÜLLER, Peter-Jürgen , ROTH, Martin , GROTH, Ingrid , KUMMER, Christel , MARTIN, Karin , SCHROECKH, Volker , METZE, Josef , KÖHLER, Michael , HENKEL, Thomas , GASTROCK, Gunter , LEMKE, Karen , HILLIGER, Matthias
摘要： The invention relates to methods for individually separating microorganisms from a suspension or culture, parallel cultivation of individual cells and analyzing the metabolic performances thereof. The invention enables basic microbial operations such as media optimization, screening according to images of novel natural substances and special metabolic performances, qualitative and quantitative detection of the effects of nutrient substrates, effectors and active substances including the media optimization and selection of microorganism clones with specific properties from large populations according to mutagenesis, transformation, transfection and genetic processing in addition to the detection of microbial contaminations to be carried out. One advantage of the invention is that it can be applied when microorganisms having outstanding properties can be respectively obtained as individual cells or individual organisms from a large population and can be characterized as pure cultures or when the effect of influencing variables can be examined in many fully comparable cultures.
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公开(公告)号：EP1425384A2
公开(公告)日：2004-06-09
申请号：EP02776674.0
申请日：2002-09-13
申请人： HANS-KNÖLL-INSTITUT FÜR NATURSTOFF-FORSCHUNG e.V. , INSTITUT FÜR BIOPROZESS- UND ANALYSENMESSTECHNIK e.V. , INSTITUT FÜR PHYSIKALISCHE HOCHTECHNOLOGIE E.V.
发明人： MÜLLER, Peter-Jürgen , ROTH, Martin , GROTH, Ingrid , KUMMER, Christel , MARTIN, Karin , SCHROECKH, Volker , METZE, Josef , KÖHLER, Michael , HENKEL, Thomas , GASTROCK, Gunter , LEMKE, Karen , HILLIGER, Matthias
摘要： The invention relates to methods for individually separating microorganisms from a suspension or culture, parallel cultivation of individual cells and analyzing the metabolic performances thereof. The invention enables basic microbial operations such as media optimization, screening according to images of novel natural substances and special metabolic performances, qualitative and quantitative detection of the effects of nutrient substrates, effectors and active substances including the media optimization and selection of microorganism clones with specific properties from large populations according to mutagenesis, transformation, transfection and genetic processing in addition to the detection of microbial contaminations to be carried out. One advantage of the invention is that it can be applied when microorganisms having outstanding properties can be respectively obtained as individual cells or individual organisms from a large population and can be characterized as pure cultures or when the effect of influencing variables can be examined in many fully comparable cultures.
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