公开(公告)号：EP2877828A1

公开(公告)日：2015-06-03

申请号：EP13739145.4

申请日：2013-07-17

申请人： Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. ,  Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V.

发明人： FUHR, Günter R. ,  ZIMMERMANN, Heiko ,  STRACKE, Frank ,  SCHMITT, Daniel ,  GRABENBAUER, Markus ,  BASTIAENS, Philippe ,  WEHNER, Frank

IPC分类号： G01N1/42 ,  A01N1/02

CPC分类号： A01N1/0289 ,  G01N1/42

摘要： The invention relates to the temperature control, in particular to the ice crystal-free freezing or thawing of a sample (1), comprising the steps of providing the sample (1) on a front side (11) of a carrier substrate (10) and supplying a temperature-control medium (2) to a back side (12) of the carrier substrate (10), wherein the temperature-control medium (2) in the above-critical state and having a pressure that is higher than the atmospheric pressure is brought into contact with the back side (12) of the carrier substrate (10) and the temperature of the sample (1) is rapidly modified. The invention further relates to a temperature control device (100) for the temperature control of a sample.

公开(公告)号：EP2877828B1

公开(公告)日：2018-07-11

申请号：EP13739145.4

申请日：2013-07-17

申请人： Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. ,  Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V.

发明人： FUHR, Günter R. ,  ZIMMERMANN, Heiko ,  STRACKE, Frank ,  SCHMITT, Daniel ,  GRABENBAUER, Markus ,  BASTIAENS, Philippe ,  WEHNER, Frank

IPC分类号： G01N1/42 ,  A01N1/02

CPC分类号： A01N1/0289 ,  G01N1/42

公开(公告)号：EP2765854A2

公开(公告)日：2014-08-20

申请号：EP12772885.5

申请日：2012-10-10

申请人： Fraunhofer Gesellschaft zur Förderung der angewandten Forschung e.V. ,  Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V.

发明人： FUHR, Günter, R. ,  ZIMMERMANN, Heiko ,  STRACKE, Frank ,  GRABENBAUER, Markus ,  HÜBINGER, Jan ,  BASTIAENS, Philippe ,  WEHNER, Frank

IPC分类号： A01N1/02

CPC分类号： F25D31/00 ,  A01N1/0268 ,  A01N1/0284 ,  A01N1/0289

摘要： A cryopreservation device (100) which is arranged for cryopreservation of a biological sample (1) comprises a pressure vessel (10) with a vessel wall (11) and an internal space (12) which is arranged to receive the biological sample (1), wherein the pressure vessel (10) is equipped with an actuating device (20) for cooling by lowering the temperature and raising the pressure in the pressure vessel (10) and configured for the cryopreservation of the biological sample (1). Said actuating device (20) is connected to the vessel wall (11) and comprises at least one pressure setting element (21 - 23, 31) and at least one of at least one cooling element (34) and at least one heat conducting element (35 - 38), wherein the actuating device (20) is configured for a time-dependent and/or location-dependent setting of the temperature and of the pressure in the pressure vessel (10). Methods are also described for cryopreservation of a biological sample (1), comprising biological cells (2) and a preservation medium (3), and methods for heating the biological sample (1) to maintain vitality.

公开(公告)号：EP2991480A1

公开(公告)日：2016-03-09

申请号：EP14718919.5

申请日：2014-04-16

申请人： Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V.

发明人： WEHNER, Frank ,  BASTIAENS, Philippe, I., H. ,  AZER, Lale ,  CHRISTMANN, Jens

IPC分类号： A01N1/02

CPC分类号： A01N1/0226

摘要： The present invention relates to the use of a signal or effector protein/peptide which affects the osmoregulation or general transporting processes of cells for cryoconservation and to a method for cryopreservation of cells using such a signal or effector protein/peptide. The claimed method comprises at least the following steps: a) contacting the cells with a signal or effector protein/peptide having at least one of the following properties : - capable to affect the osmoregulation of cells, - capable to affect general transporting processes in cells which result in notable differences of the physiological parameters of cooled cells before and after freezing and/or of the distribution of osmolytes and water in said cells, - capable to affect regulatory processes which involve membrane-bound transporters, for a predetermined time which is sufficient to allow the signal or effector protein/peptide to display a cryoprotective effect on said cells, and b) cooling the cells to a target temperature below 0°C. More specifically, the signal or effector protein/peptide is selected from the group comprising antidiuretic hormones, in particular vasopressin and its derivatives, aldosteron and its derivatives, cytokines, in particular TNF-α, that affect hypertonicity-induced cation channels (HICCs).