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1.发明授权Method for the production of recombinant flavivirus, DNA constructs, recombinant flavivirus and vaccine compositions 有权一种用于生产重组黄病毒，DNA构建体，重组黄病毒和疫苗组合物的过程中
公开(公告)号：EP1989316B1
公开(公告)日：2015-04-01
申请号：EP06804595.4
申请日：2006-10-31
CPC分类号： A61K39/12 , A61K2039/525 , A61K2039/5256 , A61K2039/575 , C07K14/005 , C07K2319/02 , C07K2319/03 , C07K2319/40 , C12N7/00 , C12N2770/24122 , C12N2770/24134 , C12N2770/24143 , C12N2770/24171 , Y02A50/386 , Y02A50/388
摘要： The purpose of the present invention is the production of recombinant virus through the cloning and expression of sequences of coding nucleotides of the whole or part of heterolog proteins, through the following method: (a) modification of the heterolog nucleotides sequences in such way they when cloned and expressed in the vector virus, they present in the 5′ region, nucleotides present in the 5′ edge of the gene NS1 of this vector virus or of other virus or equivalent functional sequences, and in its 3′ region, the correspondent genome region in the whole or part of the spheres of the steam and anchor of the protein E of this vector virus or equivalent functional sequences, and not compromising the structure and the replication of the mention vector virus; (b) insertion of the modified heterolog sequences in (a) in the intergene region at the structural protein E level and of on structural NS1 vector virus; (c) obtention of the non pathogenic recombinant virus and owner of the immunologic properties, having the heterolog sequences integrated in the viral genome according to the insertion described in (b) and, like that, expressing the heterolog antigene in such way that it can induce an appropriate immune response. The present invention is also addressed to vaccine compositions to immune against the Flavivirus and/or other pathogens.
摘要翻译： 本发明的目的是通过编码全部或异源蛋白质的一部分的核苷酸，通过以下方法的序列的克隆和表达产生重组病毒组成：（a）在寻求方式他们当异源的核苷酸序列的修饰 克隆并在所述载体的病毒，在存在于5“区的核苷酸存在于5”基因此载体病毒的病毒或其它病毒或等价的功能序列的NS1的边缘过度表达，并在其3“区域，对应的基因组区 在整个或蒸汽和该载体病毒或等效的功能序列，并且不包含所述结构和所述病毒载体提及的复制的蛋白E的锚的球体的一部分; （B）在修改的异源序列插入的（a）中在结构蛋白E水平和constructural NS1病毒载体的基因间区域; （C）的非致病性的重组病毒和免疫学特性的所有者的获得方法，其具有集成在病毒基因组雅丁到在（b）和，这样，表达在寻求方式所述异源抗原做到了可以描述插入的异源序列 诱导到适当的免疫应答。 因此，本发明致力于疫苗组合物对抗黄病毒和/或其它免疫patogens。
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2.发明公开METHOD FOR THE PRODUCTION OF RECOMBINANT VIRUS, DNA CONSTRUCTS, RECOMBINANT VIRUS AND VACCINE COMPOSITIONS 有权一种用于生产重组黄病毒，DNA构建体，重组黄病毒和疫苗组合物的过程中
公开(公告)号：EP1989316A2
公开(公告)日：2008-11-12
申请号：EP06804595.4
申请日：2006-10-31
CPC分类号： A61K39/12 , A61K2039/525 , A61K2039/5256 , A61K2039/575 , C07K14/005 , C07K2319/02 , C07K2319/03 , C07K2319/40 , C12N7/00 , C12N2770/24122 , C12N2770/24134 , C12N2770/24143 , C12N2770/24171 , Y02A50/386 , Y02A50/388
摘要： The purpose of the present invention is the production of recombinant virus through the cloning and expression of sequences of coding nucleotides of the whole or part of heterolog proteins, through the following method: (a) modification of the heterolog nucleotides sequences in such way they when cloned and expressed in the vector virus, they present in the 5′ region, nucleotides present in the 5′ edge of the gene NS1 of this vector virus or of other virus or equivalent functional sequences, and in its 3′ region, the correspondent genome region in the whole or part of the spheres of the steam and anchor of the protein E of this vector virus or equivalent functional sequences, and not compromising the structure and the replication of the mention vector virus; (b) insertion of the modified heterolog sequences in (a) in the intergene region at the structural protein E level and of on structural NS1 vector virus; (c) obtention of the non pathogenic recombinant virus and owner of the immunologic properties, having the heterolog sequences integrated in the viral genome according to the insertion described in (b) and, like that, expressing the heterolog antigene in such way that it can induce an appropriate immune response. The present invention is also addressed to vaccine compositions to immune against the Flavivirus and/or other pathogens.
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