公开(公告)号：EP2638393A4

公开(公告)日：2016-06-01

申请号：EP11839117

申请日：2011-11-09

申请人： GEN HOSPITAL CORP ,  MASSACHUSETTS INST TECHNOLOGY ,  UNIV ILLINOIS ,  DAKTARI DIAGNOSTICS INC

发明人： WATKINS NICHOLAS ,  BASHIR RASHID ,  RODRIGUEZ WILLIAM ,  CHENG XUANHONG ,  TONER MEHMET ,  CHEN GRACE ,  OPPENHEIMER AARON

IPC分类号： G01N33/53 ,  C12M1/34 ,  G01N15/10 ,  G01N15/12 ,  G01N15/14 ,  G01N21/17 ,  G01N33/52 ,  G01N33/543 ,  G01N33/569

CPC分类号： G01N27/02 ,  B01L2200/0652 ,  B01L2300/0877 ,  C12M41/36 ,  G01N15/12 ,  G01N15/1245 ,  G01N33/56972 ,  G01N2015/1006 ,  G01N2015/1486

摘要： This disclosure relates to methods and devices to count particles of interest, such as cells. The methods include obtaining a fluid sample that may contain particles of interest; counting all types of particles in a portion of the sample using a first electrical differential counter to generate a first total; removing any particles of interest from the portion of the fluid sample; counting any particles remaining in the portion of the fluid sample using a second electrical differential counter after the particles of interest are removed to generate a second total; and calculating a number of particles of interest originally in the fluid sample by subtracting the second total from the first total, wherein the difference is the number of particles of interest in the sample. These methods and related devices can be used, for example, to produce a robust, inexpensive diagnostic kit for CD4+ T cell counting in whole blood samples.

摘要翻译： 本公开涉及计算感兴趣的颗粒，例如细胞的方法和装置。 所述方法包括获得可含有感兴趣的颗粒的流体样品; 使用第一电差分计数器对样品的一部分中的所有类型的颗粒计数以产生第一总数; 从所述流体样品的所述部分去除任何感兴趣的颗粒; 在除去感兴趣的颗粒之后，使用第二电差速计数器对流体样品部分中残留的任何颗粒进行计数以产生第二总数; 以及通过从所述第一总数中减去所述第二总计来计算最初在所述流体样本中的感兴趣的粒子数，其中所述差是所述样本中所关注的粒子的数量。 这些方法和相关装置可用于例如在全血样品中产生用于CD4 + T细胞计数的稳健且廉价的诊断试剂盒。